Thermo Fisher Scientific ULT Manuale del proprietario

Marca: Thermo Fisher Scientific

Modello: ULT

Tipo: Manuale del proprietario

Isotemp -86°C Freezers

Installation and Operation

329712H06 • Revision C • October 2019

IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in

damage to the unit, injury to operating personnel, and poor equipment performance.

CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.

Material in this manual is for informational purposes only. The contents and the product it describes are

subject to change without notice. The Fisher ScientificTM channel makes no representations or warranties with

respect to this manual. In no event shall the Fisher ScientificTM channel be held liable for any damages, direct

or incidental, arising from or related to the use of this manual.

Contents

Models......................................................................... 1

Safety Considerations .................................................. 2

Unpacking ................................................................... 3

Packing List ............................................................. 3

General Recommendations.......................................... 4

Temperature Monitoring ........................................... 4

General Usage ......................................................... 4

Initial Loading ........................................................... 4

Battery Door Opening/Closing.................................. 4

Operating Standards.................................................... 5

Electrical Specifications............................................ 5

Installation.................................................................... 6

Location................................................................... 6

Leveling.................................................................... 6

Backup System (Optional) ........................................ 6

Super Insulated Cabinet Construction ...................... 6

Door Operation ........................................................ 6

Pressure Equalization Port........................................ 7

Installing the Remote Alarm Connector..................... 7

Intended Use............................................................ 7

Operation..................................................................... 9

Initial Start Up........................................................... 9

Operation Overview.................................................. 9

Display ..................................................................... 9

Settings.................................................................... 10

Power Down ............................................................ 11

Backup System (Optional)............................................ 12

CO2 and LN2 Precautions ....................................... 12

Installation................................................................ 12

Start Up ................................................................... 13

Operation................................................................. 13

Chart Recorders (Optional)........................................... 14

Set Up and Operation .............................................. 14

Changing Chart Paper.............................................. 14

Calibration Adjustment ............................................. 14

Maintenance ................................................................ 15

Cleaning the Condenser........................................... 15

Cleaning the Condenser Filter................................... 15

Gasket Maintenance ................................................ 15

Defrosting the Freezer .............................................. 15

Battery Maintenance ................................................ 15

Maintenance Schedule............................................. 16

Troubleshooting Guide................................................. 17

Error Codes ................................................................. 20

Warranty...................................................................... 21

Appendix A: Alarm Summary ....................................... 22

Contact Information .................................................... 25

Isotemp -86°C Freezers Models | 1

Models

Table 1. Applicable Models

Brand - Model Size (xxx) Voltage (*)

Isotemp - IUExxx86L* 300/400/500/600 A/D/V

2 | Safety Considerations Isotemp -86°C Freezers

Safety Considerations

In this manual, the following symbols and conventions are

used:

Below are important safety precautions that apply to this

product:

EMC

EMC Registration is done on this equipment for business use

only. It may cause interference when the product would be

used in home.

사용자 안내문 이 기기는 업무용 환경에서 사용할 목

적으로 적합성평가를 받은 기기로서 가정용 환경에서

사용하는 경우 전파간섭의 우려가 있습니다 .

This equipment has been tested and found to comply with the

limits for a Class A digital device. Class A covers devices for

usage in all establishments other than domestic and that are

not directly connected to a low voltage power supply

network, which supplies domestic environment.

This ISM device complies with Canadian ICES-001.

FCC

This equipment has been tested and found to comply with the

limits for a Class A digital device, pursuant to part 15 of the

FCC Rules. These limits are designed to provide reasonable

protection against harmful interference when the

equipment is operated in a commercial environment. This

equipment generates, uses, and can radiate radio frequency

energy and, if not installed and used in accordance with the

instruction manual, may cause harmful interference to radio

communications. Operation of this equipment in a residential

area is likely to cause harmful interference in which case the

user will be required to correct the interference at his own

expense.

This symbol used alone indicates important

operating instructions which reduce the risk of

injury or poor performance of the unit.

CAUTION: This symbol, in the context of a

CAUTION, indicates a potentially hazardous

situation which if not avoided could result in

minor to moderate injury or damage to the

equipment.

WARNING: This symbol indicates potentially

hazardous situations which, if not avoided, could

result in serious injury or death.

WARNING: This symbol indicates situations

where dangerous voltages exist and potential for

electrical shock is present.

The snowflake symbol indicates extreme low

temperatures and high risk of frostbite. Do not

touch bare metal or samples with unprotected

body parts.

This symbol indicates a need to use gloves during

the indicated procedures. If performing

decontamination procedures, use chemically

resistant gloves. Use insulated gloves for handling

samples and when using liquid nitrogen.

Before installing, using or maintaining this

product, please be sure to read this manual and

product warning labels carefully. Failure to follow

these instructions may cause this product to

malfunction, which could result in injury or

damage.

Use this product only in the way described in the

product literature and in this manual. Before using

it, verify that this product is suitable for its

intended use. If this equipment is used in a

manner not specified by the manufacturer, the

protection provided by the equipment may be

impaired.

Do not modify system components, especially

the controller. Use OEM exact replacement

equipment or parts. Before use, confirm that the

product has not been altered in any way.

WARNING: Your unit must be properly grounded

in conformity with national and local electrical

codes. Never connect the unit to overloaded

power sources.

WARNING: Disconnect the unit from all power

sources before cleaning, troubleshooting, or

performing other maintenance on the product or

its controls.

WARNING: “Caution, risk of fire”. This unit is

charged with hydrocarbon refrigerants.

Isotemp -86°C Freezers Unpacking | 3

Unpacking

At delivery, examine the exterior for physical damage while

the carrier’s representative is present. If exterior damage is

present, carefully unpack and inspect the unit and all

accessories for damage.

If there is no exterior damage, unpack and inspect the

equipment within five days of delivery. If you find any damage,

keep the packing materials and immediately report the

damage to the carrier. Do not return goods to the

manufacturer without written authorization. When submitting

a claim for shipping damage, request that the carrier inspect

the shipping container and equipment.

The packaging can be stored and re-used.

Packing List

Inside the freezer cabinet is a bag containing:

• A handle lock key

• A USB drive with user manual, including translated

versions

• Certificates of conformance and calibration

• A remote alarm contact connector

• Posts for rear spacing

If you have ordered a chart recorder, the bag will also contain:

• Recorder installation instructions

• Extra paper

If you have ordered a backup system, the cabinet will also

contain:

• A hose assembly

• English and metric connectors

If specified on the order, the bag may also include:

• A QC temperature graph and test log

• Calibration information

4 | General Recommendations Isotemp -86°C Freezers

General Recommendations

Temperature Monitoring

General Usage

This refrigeration system is designed to maintain ultra-low

temperatures with safety in an ambient environment within

15°C to 32°C (59°F to 90°F), only when the freezer is used for

storage.

Avoid opening the door for extended time periods since

chamber temperature air will escape rapidly. Also, keep the

inner doors closed as much as possible. When room air,

which is higher in humidity, replaces chamber air, frost may

develop in the chamber more rapidly.

Initial Loading

Allow the freezer to operate at the desired temperature

for a minimum of 12 hours before loading.

Load the freezer one shelf at a time, beginning with the top

shelf. After loading each shelf, allow the freezer to recover to

the desired set point before loading the next shelf. Repeat this

process until the freezer is fully loaded.

Battery Door Opening/

Closing

To open the grille door, pull the door from the top right corner

as shown in the figure below.

To close the grille door, push the door against frame to hold

latch in position.

Figure 1. Door Opening

IMPORTANT NOTE: The Fisher ScientificTM

channel recommends the use of a redundant and

independent temperature monitoring system so

that the freezer can be monitored continuously

for performance commensurate with the value of

product stored.

WARNING: This unit is not a “rapid-freeze”

device. Freezing large quantities of liquid, or high-

water content items, will temporarily increase the

chamber temperature and will cause the

compressors to operate for a prolonged time

period.

CAUTION: Failure to follow these procedures or

overloading the unit may cause undue stress on

the compressors or jeopardize user product

safety.

Isotemp -86°C Freezers Operating Standards | 5

Operating Standards

The freezers described in this manual are classified for use as

stationary equipment in a Pollution Degree 2 and Over voltage

Category II environment.

These units are designed to operate under the following

environmental conditions:

• Indoor use

• Altitude up to 2000 m

• Maximum relative humidity 60% for temperatures within

15°C to 32°C (59°F to 90°F)

• Main supply voltage fluctuations not to exceed ±10% of

the nominal voltage for 115 V/60 Hz & 230 V/50 Hz

• Main supply voltage fluctuations not to exceed -10% or

+6% of the nominal voltage for 208-230 V/60 Hz

Electrical Specifications

The last character in the model number listed on the data-

plate identifies the electrical specifications for your unit.

Specific unit current rating is listed on the data-plate.

The voltage types are A, D and V as specified in the following

table:

* Values subject to change

Table 2. Electrical Specifications by Size and

Voltage

Size/

Voltage Voltage Frequency Current*

300A 115 V 60 Hz 12.6 A

300D 208-230 V 60 Hz 5.6 A

300V 230 V 50 Hz 5.9 A

400A 115 V 60 Hz 13.0 A

400D 208-230 V 60 Hz 5.9 A

400V 230 V 50 Hz 5.3 A

500A 115 V 60 Hz 12.5 A

500D 208-230 V 60 Hz 5.0 A

500V 230 V 50 Hz 5.9 A

600A 115 V 60 Hz 12.7 A

600D 208-230 V 60 Hz 6.2 A

600V 230 V 50 Hz 6.1 A

Table 2. Electrical Specifications by Size and

Voltage

6 | Installation Isotemp -86°C Freezers

Installation

Location

Install the unit in a level area free from vibration with a

minimum of 8" (20 cm) of space on the top and sides, 6" (15

cm) in back. Refer to Leveling for further instructions on

leveling cabinets. Allow enough clearance so that door can

swing open at least 85°.

The rear spacing posts provided with the freezer can be used

to ensure proper clearance. To install the spacing posts,

screw them into the back side of the unit, in the rear deck

area.

Do not position the equipment in direct sunlight or near

heating diffusers, radiators, or other sources of heat. The

ambient temperature range at the location must be 15°C to

32°C (59°F to 90°F).

Wiring

Leveling

Make sure that the floor is level. The unit must be level both

front to back and side to side.

The 300 and 400 box capacity models are equipped with one

or two leveling legs on the right hand side. Leveling legs on

300 size must be used as a safety precaution.

Ensure to lock the brakes for units equipped with casters.

Backup System (Optional)

If you are using a CO2 or LN2 backup system, refer to

Backup System (Optional) for installation and operation

instructions.

Super Insulated Cabinet

Construction

In all models, the cabinet walls have a vacuum insulation core

encapsulated by a sealed film laminate.

Door Operation

Upright freezer models are equipped with an advanced

assembly specifically designed for ultra-low temperature

freezers.

Features include:

• One-hand operation

• A front-accessible lock

WARNING: Do not exceed the electrical rating

printed on the data plate located on the lower

left side of the unit.

CAUTION: Connect the equipment to the

correct power source. Incorrect voltage can

result in severe damage to the equipment.

CAUTION: For personal safety and trouble-free

operation, this unit must be properly grounded

before it is used. Failure to ground the equipment

may cause personal injury or damage to the

equipment. Always conform to the National

Electrical Code and local codes. Do not connect

the unit to overloaded power lines.

CAUTION: Do not position the unit in a way that

impedes access to the disconnecting device or

circuit breaker in the back of the unit.

CAUTION: Always connect the freezer to a

dedicated (separate) circuit. Each freezer is

equipped with a service cord and plug designed

to connect it to a power outlet which delivers the

correct voltage. Supply voltage must be within

±10% of the freezer rated voltage for 115 V/

60 Hz & 230 V/50 Hz. Supply voltage must be

within -10% to +6% of the freezer rated voltage

for 208-230 V/60 Hz. If cord becomes damaged,

replace with a properly rated power supply cord.

Table 3. Power Cord Specifications

Model Power Cord Specification

A3-G 12 AWG, NEMA 5-20P, 20A/125V

D3-G 12 AWG, NEMA 6-15P, 15A/250V

V3-G 1.5 mm, CEE 7/7, 16A/250V

CAUTION: Never remove or disable the

grounding prong from the service cord plug. If the

prong is removed, the warranty is invalidated.

CAUTION: Never drill holes in or near the cabinet

walls. Drilling could damage the insulation and

make the unit inoperable.

Isotemp -86°C Freezers Installation | 7

• Hasps for a standard padlock to provide additional

security. Length of the shackle must be between 3/4"

(1.9 cm) and 1 1/2" (3.8 cm)

• Durable construction for reliable operation and safe

product storage

• Door ramp alignment feature

Opening the Door

1. Remove the padlock if installed.

2. Grasp the latch handle and pull it toward yourself until the

latch disengages.

3. Keep pulling by the latch handle to open the main door.

Closing the Door

Note: The latch does not self-engage automatically when you

close the door. You must rotate the latch into the open

position first.

1. Grasp the latch handle and pull it toward yourself,

rotating the latch into the open position.

2. Move the freezer door into the closed position and gently

push the handle away from you, making sure that the

latch engages fully with the cabinet strike.

3. Keep applying gentle pressure to the latch handle until

the latch is securely in closed position.

4. Insert the key and rotate counterclockwise to lock.

5. Replace the padlock as required.

Pressure Equalization Port

When an upright ultra-low temperature freezer door is

opened, room temperature air rushes into the storage

compartment. When the door is closed, the fixed volume of

air is cooled rapidly. Pressure drops below atmospheric

pressure, resulting in a substantial vacuum. Re-entry into the

cabinet is impossible until internal pressures are returned to

atmospheric pressure. Without a pressure equalization

mechanism, it can take, in extreme cases, several hours

before the door can easily be reopened.

All upright models feature a port that provides vacuum relief

after door openings. The pressure equalization port is located

in the door behind the eye-level panel on the front of the

freezer. Although the port is heated and designed to self-

defrost, excessive frost accumulation on the inner door could

eventually restrict air flow. Therefore, you should periodically

inspect the inner door and brush away any loose frost using a

stiff nylon brush.

Installing the Remote

Alarm Connector

The remote alarm contacts are located on the back of the

freezer above and to the left of the power switch. After

installing the wiring from the remote alarm to the connector,

install the connector to the freezer micro-board.

The pin configuration is shown in Figure 2 below.

Figure 2. Remote Alarm Pin Configuration

For systems that alarm in closed state, connect to pins 5 & 6.

For systems that alarm in open state, connect to pins 6 & 7.

The contacts will trip in the event of a power outage, high

temperature alarm, low temperature alarm or door ajar alarm.

Intended Use

The –86°C freezers (refer to Models for the specific model

series) described in this manual are intended for the storage

of Frozen Red Blood Cells in 40% glycerol at -65°C for up to

10 years; Frozen Rejuvenated Red Blood Cells at -65°C for

up to 10 years; Fresh Frozen Plasma at -65°C for up to 10

years; and Frozen or Cryopreserved Tissue for long term

storage: Musculoskeletal Tissue at -40°C or colder, Skin at

-40°C or colder and Bone at -40°C or colder.

These products should only be used by an authorized and

adequately trained personnel. It is considered a medical

device and has therefore been listed with medical device

regulatory body FDA and is considered a Class II medical

device by the FDA. This product is listed under product code

KSE, regulation number 864.9700 and is considered 510(K)

exempt. This unit is not intended for use in classified

hazardous locations, nor to be used for the storage of

flammable inventory.

CAUTION: When moving the freezer, always

grasp cabinet surfaces; never pull the freezer by

the latch handle.

8 | Installation Isotemp -86°C Freezers

Reference: AABB - Technical Manual 17th Ed., page

273,274.

STANDARDS FOR TISSUE BANKING 14th Ed., Section E,

E3.300, E3.310, Table E3.331.

Note for clarity:

The ‘Bone’ is the Osteoarticular Graft, a weight bearing

allograft with intact articular surfaces, consisting of a joint with

associated soft tissue and bone. STANDARDS FOR TISSUE

BANKING 14th Ed., page 8.

Isotemp -86°C Freezers Operation | 9

Operation

Initial Start Up

To start the freezer, complete the following steps:

1. Plug the freezer into the power outlet.

2. Turn the power switch ON. You can find the switch in

back of the freezer, on the bottom right.

3. Once the freezer is turned ON, the user interface will

begin a start up procedure. Once ready for operation, the

temperature is displayed on the screen.

Operation Overview

Once you have successfully completed the initial start up

procedures, the freezer starts operating normally and the only

actions required are:

• Setting the operating and alarm set points.

• Activating the CO2 or LN2 backup system if installed. For

instructions on backup settings and activating the

system, refer to Backup System (Optional).

Display

The display screen below is the default screen.

Figure 3. Display

The control panel consists of the 5 touch-point buttons

located on the right side of the display.

• Warm Alarm value - The range of the warm alarm

temperature is –40°C to within 5°C of setpoint.

Note : The warm alarm will be disabled for 12 hours

from a warm start condition.

• Cold Alarm value - The range is –99°C to within 5°C

of setpoint.

Note : A setpoint change may automatically change

the warm/cold alarm setpoints as well to maintain a

minimum 5°C separation from the control setpoint.

•Offset value - This is used for calibration. Range is

–10°C to +10°C. Default is 0.

Entering a positive offset value will yield a colder

cabinet temperature.

Entering a negative offset value will yield a warmer

cabinet temperature.

•Setpoint security code – This code is a 3-digit

numeric code. Refer to Setpoint Security.

•Backup system type (if backup system is installed)

– Set the type to either LN2 or CO2 corresponding to

the backup system that is installed.

•Backup system setpoint (if backup system is

installed) - This setpoint indicates the temperature at

which the backup system will begin cooling the

cabinet. It is recommended to set the backup system

setpoint at a minimum of 10°C warmer than the

control setpoint. For more information, refer to

Backup System (Optional).

1. Alarm Bell – This icon indicates visual and

audible alarm that accompanies various alarm

states. Pressing the alarm bell while in an alarm

state will snooze the audible alarm for 10

minutes.

2. Plus – Increases the value of the selected

setting.

3. Check Mark – Saves a change to the selected

value.

4. Minus – Decreases the value of the selected

setting.

5. Settings – The settings icon represents the

various settings including:

10 | Operation Isotemp -86°C Freezers

The message panel on the top indicates freezer health status

and the various alarm or warning states.

Settings

If setpoint security is enabled, you must first enter the security

code to make any setpoint changes. Refer to Setpoint

Security to adjust setpoint security.

Control Setpoint

To modify the control temperature setpoint:

• Press the plus or minus button while displaying unit

temperature (“Actual” is illuminated). The control setpoint

is displayed

• Adjust setpoint to desired temperature

• Select the checkmark button to save the new control

setpoint.

Other Setpoints and Settings

• Press the settings button to enter Settings menu

• Continue pressing the settings button until the desired

setting is illuminated on lower horizontal panel. (If backup

system is installed, CO2 or LN2 is displayed after pressing

the settings button 5 times.)

• Adjust the setting to desired temperature or value using

the plus or minus buttons

• Press the checkmark button to save the new

temperature or value

• After the value is saved, display will show the next option

in the settings menu.

To return to the unit temperature display:

• Press the settings button until unit temperature is

displayed (“Actual” is illuminated)

• If there is no activity after 5 minutes, the unit will

automatically return to the temperature display.

Warm Alarm Test

Press the plus and checkmark buttons at the same time to

initiate the warm alarm test. During the warm alarm test, the

actual cabinet temperature will not be displayed. The display

temperature will increase. Once the display temperature

reaches the warm alarm setpoint, the alarm is activated. After

5 seconds, the test will automatically end and the display will

return to the actual cabinet temperature.

Setpoint Security

• To adjust setpoint security in the settings menu, press the

settings button 4 times

• The setpoint security code consists of 3 digits, each of

which must be set in sequence from left to right

• Use the plus or minus button to adjust each value, and

the checkmark button to save each value of the 3-digit

security code

• If you forget the setpoint security code, contact customer

support.

1. Wrench – This is a generic service warning which

corresponds to an intermittent flashing error code

displayed on the screen. Refer to Error Codes

for a list of error codes.

2. Door – This icon will illuminate during a door ajar

alarm. A door open for more than 3 minutes will

result in an audible door ajar alarm.

3. Shield – The shield is the health status for the

freezer. A green shield indicates normal freezer

operation. In an alarm state, this icon is not

illuminated.

4. Thermometer – This indicates when the cabinet

temperature exceeds either warm alarm or cold

alarm setpoints and the audible alarm will occur.

5. Snooze Bell – This is only illuminated during an

active alarm that has been silenced by the user.

Isotemp -86°C Freezers Operation | 11

Power Down

To power down the ULT, first turn the breaker switch, located

at the rear of the freezer, to the off position. Once the switch

is in the off position, the display will show "OFF" followed by

"YES" and then "NO" in 2 second intervals. The checkmark

will also be illuminated while "YES" and "NO" are showing.

Press the checkmark while "YES" is showing. With a flashing

"YES", you are required to confirm by pressing the checkmark

button a second time. Power down will then be complete.

If the checkmark button is pressed while "NO" is illuminated

or if no action is taken for 5 minutes, this is interpreted as a

power failure. In this case, the user interface will stay on (using

battery power only) and an audible alarm will sound to

indicate power failure. If installed, the backup system will

remain active and inject per the backup system settings.

12 | Backup System (Optional) Isotemp -86°C Freezers

Backup System (Optional)

For all ultra-low temperature cold storage products, we

recommend the use of a backup system (BUS) for the

security of your samples.

When you purchase a built-in CO2 or LN2 optional backup

system for the freezer, backup control is integrated into the

main user interface.

Note: For stand-alone backup systems, refer to installation

instructions provided with the backup system kit.

CO2 and LN2 Precautions

The following are precautions for using liquid CO2 and LN2

backup systems.

Installation

Field installed systems are supplied with complete installation

and operating instructions. If your system is factory installed,

the freezer is shipped with a coiled length of hose to connect

the freezer to the bottles:

• 1/4” Flexible Hose with fittings for connection to the CO2

supply.

• 1/2” Flexible Hose with fittings for connection to the LN2

supply.

To install,

1. Straighten the coiled hose.

2. Connect one end to the labeled connection on the

freezer.

• Tighten the nut two flats past finger tight, approximately

120 degrees.

Note: For CO2, remove the threaded fitting from the nut on

the end of the copper tubing to access nut for connection to

the freezer. Discard the threaded fitting.

3. Attach the other end to the supply bottle or building

supply fitting.

•For CO

• Remove Nipple from adapter (NPT Connection).

Remove cable tie to release alternative nut and

washer. Ensure the correct nut fitting is supplied over

the nipple (US or European).

Note: Always purchase the cylinders which are

equipped with siphon tubes for withdrawing liquid

from the bottom of the cylinder. CO2 cylinders

must be kept at room temperature to function

properly. LN2 bottles are functional at any

reasonable temperature.

WARNING: If a CO2 or LN2 cylinder falls and a

valve is knocked off, the cylinder becomes a

deadly and completely unguided missile.

Transport the cylinders in a hand-truck or cart

with secure chain ties for the cylinder. After

cylinders are connected to the equipment,

securely attach them with chains to a solid

stationary object such as a building column.

WARNING: CO2 and LN2 liquids are non-

poisonous but are very cold and will burn

unprotected skin. Always wear protective eye

wear and clothing when changing cylinders or

working on the piping systems attached to an

active source of liquid refrigerant.

WARNING: The gases produced by evaporation

of CO2 or LN2 are non-poisonous but displace

the oxygen in a confined space and can cause

asphyxiation. Do not store the cylinders in

subsurface or enclosed areas.

CAUTION: When closing the cylinder valve,

make sure that the injection solenoid is energized

to allow all the liquid to bleed off instead of being

trapped in the supply hose. Failure to do this

results in activation of the pressure relief device,

which could damage the freezer and requires

replacing if it is activated.

CAUTION: For models ordered with factory

installed built-in backup systems, the flow of

liquid CO2 or LN2 will be discontinued if the door

is opened during operation of the backup

system. For units operated with free-standing,

field installed type backup system, the flow of

liquid CO2 or LN2 will be discontinued upon door

opening only if the switch provided with the free-

standing package is installed on the freezer.

Isotemp -86°C Freezers Backup System (Optional) | 13

• Add 2 wraps of Teflon tape clockwise to the 1/4”

NPT fitting (on the nipple) when viewed from the

threads. Tighten the NPT fittings approximately 2

turns from finger tight (approximately 720°).

Note : The top of the nipple has a hex configuration,

allowing for use of a wrench when the nut is pulled

down.

• Add washer to nipple inside of nut (unless CO2

supply has a built in washer).

Note : Small raised area of washer fits into groove of

nipple. The washer will feel snug when trying to shift

side-to-side on nipple. The washers are designed for

a limited number of attachments/disconnections

from the supply and may wear over time. If washer

appears worn and causes CO2 leakage, replace

washer (Part Number 45705H03).

• Wrench tighten the supply nut to the supply.

•For LN

• Attach the fitting to the supply and wrench tighten.

Note: Do not twist, torque, or subject the flexible hose to

sharp bends. Doing so may shorten the life of the hose.

Start Up

When the unit is started, it will recognize if a backup system is

installed.

1. Follow the instructions in Backup System (Optional) to

set the backup system type and setpoint.

2. It is recommended to test backup system operation prior

to sample storage.

Test BUS Operation

After the freezer has stabilized and both batteries are fully

charged, the BUS can be tested to verify proper operation.

1. Disconnect the AC power to the freezer by turning the

power switch off.

2. As the freezer warms up, verify the BUS injects at the

desired temperature. Displayed temperature may vary by

a few degrees from injection temperature due to the

differences in probe locations.

Note: On a monthly basis, it is recommended to test your

backup system, check the supply tank system levels, and

check the backup battery voltage.

Operation

The backup system can run for a minimum of 24 hours on

battery power.

On average, a backup system in operation uses 8 to 10 lbs.

per hour of CO2 (3.6 to 4.5 L/hr) or LN2 (4.5 to 5.6 L/hr) at an

ambient temperature of 25°C.

This rate will vary depending on set point, load, ambient

temperature and freezer size.

14 | Chart Recorders (Optional) Isotemp -86°C Freezers

Chart Recorders (Optional)

Panel-mounted six-inch seven-day recorders are available as

options for all freezer models except for the smaller (300 and

400 box capacity) models.

Set Up and Operation

To prepare the recorder to function properly, complete the

following steps:

1. Open the grille door to access the recorder.

2. Install clean chart paper (refer to Changing Chart

Paper).

3. Remove the plastic cap from the pen stylus or ink pen

and close the recorder door.

Recorder operation begins when the system is powered on.

The recorder may not respond until the system reaches

temperatures within the recorder’s range.

Figure 4. Chart Recorder

Figure 5. Chart Buttons

Changing Chart Paper

To change the chart paper, complete the following steps:

1. Locate the pressure sensitive buttons at the front, upper

left of the recorder panel.

2. Press and hold the Change Chart button (#3) for one

second. The pen will move off the scale.

3. Unscrew the center nut, remove the old chart paper, and

install new chart paper. Carefully align the day and time

with the reference mark (a small groove on the left side of

the recorder panel).

4. Replace the center nut and hand tighten. Press the

Change Chart button again to resume temperature

recording.

Calibration Adjustment

This recorder has been accurately calibrated at the factory

and retains calibration even during power interruptions. If

required, however, adjustments can be made as follows:

1. Run the unit continuously at the control set point

temperature. Continue steady operation for at least two

hours to provide adequate time for recorder response.

2. Measure cabinet center temperature with a calibrated

temperature monitor.

3. Compare the recorder temperature to the measured

cabinet temperature. If necessary, adjust recorder by

pressing the left (#1) and right (#2) chart buttons.

Note: The stylus does not begin to move until the top center

button (#3) is held for five seconds.

CAUTION: Do not use sharp or pointed objects

to depress the chart buttons. This may cause

permanent damage to the recorder.

Isotemp -86°C Freezers Maintenance | 15

Maintenance

Cleaning the Condenser

Clean the condenser at least every six months; more often if

the laboratory area is dusty.

To clean the condenser, complete the following steps:

1. Pull the grille door open.

2. Vacuum the condenser.

3. Inspect the filter cleanliness and clean as required.

4. Close the grille door.

Cleaning the Condenser

Filter

Clean the condenser filters every two or three months.

1. Pull the grille door open.

2. Remove the filter.

3. Shake the filter to remove loose dust, rinse the filters in

clean water, shake the excess water from the filter, and

replace the filter.

4. Close the grille door.

Gasket Maintenance

Periodically check the gaskets around the door for punctures

or tears. Leaks are indicated by a streak of frost which forms

at the point of gasket failure. Make sure that the cabinet is

level (refer Leveling for leveling information).

Keep the door gaskets clean and frost free by wiping gently

with a soft cloth.

Defrosting the Freezer

Defrost the freezer once per year or whenever the ice buildup

exceeds 3/8”. To defrost, complete the following steps:

1. Remove all products and place in another ULT freezer.

2. Turn off the freezer.

3. Open the outer door and all inner doors.

4. Let the freezer stand with doors open for at least 24

hours. This allows both the interior and foamed

refrigeration system to warm to room temperature.

5. Dispose of the ice and wipe out any water standing in the

bottom of the cabinet.

6. If there is freezer odor, wash the interior with a solution of

baking soda and warm water.

7. Clean the exterior with any common household cleaner.

8. Close the doors, restart the freezer and reload. Refer

Initial Loading to follow the instructions.

Battery Maintenance

The freezer monitors the voltage status of the battery daily

and indicates the battery’s voltage via visual and auditory

alarm. Replace the battery as indicated by system alarms or

as necessary per individual status evaluation. Check the

battery connections regularly. Although not required, annual

battery replacement is recommended to ensure proper

battery status in the event of power failure.

For safety, it is recommended to power off the unit and

disconnect it from the power source before replacing the

battery. Battery terminals are color coded in red and black.

Ensure the corresponding colored wires are connected to the

matching color terminals on the battery. The battery is

installed with terminals oriented toward the condenser

compartment or hinge side of the freezer’s outer door (refer

Battery Specification). With proper installation, the red wire

should be connected to the rear battery (positive) terminal and

the black wire to the front (common) terminal.

Failure to properly connect the battery can damage electrical

components and potentially hinder normal operation of the

freezer. Consult a certified service technician if there are any

questions or concerns about battery maintenance.

WARNING: Unauthorized repair of your freezer

will invalidate your warranty. Contact Technical

Service. See Contact Information for phone

numbers.

CAUTION: Maintenance should only be

performed by trained personnel.

16 | Maintenance Isotemp -86°C Freezers

Battery Specification:

Rechargeable sealed lead-acid battery, 12 V, 7.0 Amp Hr.

Replacement batteries can be purchased directly from the

Fisher Scientific channel.

Figure 6. Battery Specification

Maintenance Schedule

Regular maintenance is important to keep the unit working

properly. Inspect/clean as directed in the manual.

Item Interval

Ice/Frost

build up

To maintain proper closure of inner

doors, remove any ice or frost build up

around the gasket, inner doors, and

breakers as necessary.

Gasket

Periodically check the gaskets around

the door for punctures or tears.

Periodically clean the ice-build up

around the gasket.

Filter Clean the condenser filter(s) every two to

three months.

Condenser Clean every six months, more often if the

laboratory area is dusty.

Battery

Replace the battery as indicated by

system alarms or as necessary per

individual status evaluation. Check the

battery connections regularly. Although

not required, annual battery replacement

is recommended to ensure proper

battery status in the event of power

failure.

Defrost Defrost the freezer once a year or when

the ice build exceeds 3/8” (0.95 cm)

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