Sanyo MCO-18AC UV Manuale utente

MCO-18AC(UV)

MCO-18AC

INSTRUCTION MANUAL

CO

2

Incubator

5-5, Keihan-Hondori 2-Chome

Moriguchi City, Osaka 570-8677 Japan

Printed in Japan

7FB6P101567000

(

29 June 2010

)

CONTENTS

INTRODUCTION P. 2

PRECAUTIONS FOR SAFE OPERATION P. 3

LABELS ON THE INCUBATORS P. 6

ENVIRONMENTAL CONDITIONS P. 7

INCUBATOR COMPONENTS P. 8

Control panel and keypad P. 10

Remote alarm terminal P. 11

INSTALLATION SITE P. 12

INSTALLATION P. 13

Connection of CO

2

gas cylinder P. 14

PREVENTING CONTAMINATION P. 15

PRECAUTIONS FOR CULTURES P. 16

START-UP OF INCUBATOR P. 17

SETTING THE CHAMBER TEMPERATURE AND CO

2

DENSITY P. 18

UV LAMP

(MCO-18AC(UV) or MCO-18AC with MCO-18UVS3)

P. 19

Precautions when using the UV lamp P. 20

Setting the UV lamp ON period P. 21

Lighting the UV lamp for 24 hours P. 22

KEY LOCK FUNCTION P. 23

WATER LEVEL SENSOR

(MCO-18AC(UV) or MCO-18AC with MCO-18UVS3)

P. 24

ALARMS, SAFETY, AND SELF-DIAGNOSIS P. 25

SETTING OF ALARM RESUME TIME P. 26

Operation after power failure P. 26

ROUTINE MAINTENANCE

Cleaning the chamber and inner attachments P. 27

Filling the humidifying pan P. 30

CALIBRATION

Temperature calibration P. 31

CO

2

calibration P. 32

TROUBLESHOOTING P. 33

DISPOSING OF THE CO

2

INCUBATOR P. 34

AUTOMATIC CO

2

CYLINDER CHANGEOVER P. 39

STACKING INCUBATORS P. 40

SPECIFICATIONS P. 42

PERFORMANCE P. 43

SAFETY CHECK SHEET P. 44

1

INTRODUCTION

■ Read this manual carefully before using the appliance and follow the instructions for safety operation.

■ Sanyo never guarantee any safety if the appliance is used for any objects other than intended use or

used by any procedures other than those mentioned in this manual.

■ Keep this manual in an adequate place to refer to it as necessary.

■ The contents of the manual will be subjected to change without notice due to the improvement of

performance or functions.

■ Contact Sanyo sales representative or agent if any page of the manual is lost or page order is incorrect.

■ Contact Sanyo sales representative or agent if any point in this manual is unclear or if there are any

inaccuracies.

■ No part of this manual may be reproduced in any form without the expressed written permission of

Sanyo.

CAUTION

SANYO guarantees the product under certain warranty conditions. SANYO in no way shall be

responsible for any loss of content or damage of content.

2

PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with this manual as it contains

important safety advice.

Items and procedures are described so that you can use this unit correctly and safely.

If the precautions advised are followed, this will prevent possible injury to the user and

any other person.

Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel

possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and

damage to the unit and associated property.

Symbol shows;

this symbol means caution.

this symbol means an action is prohibited.

this symbol means an instruction must be followed.

Be sure to keep this manual in a place accessible to users of this unit.

< Label on the unit >

This mark is labeled on the cover in which the electrical components of high voltage

are enclosed to prevent the electric shock.

The cover should be removed by a qualified engineer or a service personnel only.

WARNING

As with any equipment that uses CO

2

gas, there is a likelihood of oxygen depletion in the

vicinity of the equipment. It is important that you assess the work site to ensure there is

suitable and sufficient ventilation. If restricted ventilation is suspected, then other methods of

ensuring a safe environment must be considered. These may include atmosphere monitoring

and warning devices.

3

WARNING

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from

turning over. If the floor is not strong enough or the installation site is not adequate, this may result

in injury from the unit falling or tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location. This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present as current leakage or electric

shock may result due to corrosion.

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not

grounded, it will be necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed. These

may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use

may cause bad effect on your health or environment.

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to

any repair or maintenance of the unit in order to prevent electric shock or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet

hand. This may cause electric shock.

4

WARNING

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of

maintenance. These may be harmful to your health.

Nev

er splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on the unit as this may cause electric shock or short circuit when

the liquid is spilled.

Nev

er bind, process, or step on the power supply cord, or never damage or break the power

supply plug. A broken supply cord or plug may cause fire or electric shock.

Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.

Nev

er disassemble, repair, or modify the unit yourself. Any such work carried out by an

unauthorized person may result in fire, or electric shock or injury due to a malfunction.

Disconnect the power supply plug if there is something wrong with the unit. Continued

abnormal operation may cause electric shock or fire.

When remov

ing the plug from the power supply outlet, grip the power supply plug, not the cord.

Pulling the cord may result in electric shock or fire by short circuit.

Disconnect the pow

er supply plug before moving the unit. Take care not to damage the power

cord. A damaged cord may cause electric shock or fire.

Disconnect the power plug when the unit is not used for long periods. Keeping the connection

may cause electric shock, current leakage, or fire due to the deterioration of insulation.

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that

children do not have access and that doors cannot be closed completely.

The disposal of the unit should

be accomplished by appropriate personnel. Remove doors to

prevent accidents such as suffocation.

Do not put the packing plastic bag within reach of children as suffocation may result.

5

CAUTION

Use a dedicated pow

er source (a dedicated circuit with a breaker) as indicated on the rating label

attached to the unit. A branched circuit may cause fire resulting from abnormal heating.

Connect the pow

er supply plug to the power source firmly after removing the dust on the plug.

A dusty plug or improper insertion may cause a heat or ignition.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

Check

the setting when starting up of operation after power failure or turning off of power

switch. The stored items may be damaged due to the change of setting.

Be careful not to tip ov

er the unit during movement to prevent damage or injury.

Prepare a safety check sheet when you request any repair or maintenance for the safety of service

personnel.

LABELS ON THE INCUBATORS

Warning and caution labels are attached to the incubator. The following table describes the labels.

This label is attached to covers that access high-voltage electrical components to

prevent electric shock. Only a qualified engineer or service personnel should be

allowed to open these covers.

This symbol indicates an ultraviolet light (UV) caution.

This symbol indicates that caution is required. Refer to product documentation for

details.

This symbol indicates a hot surface.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

6

ENVIRONMENTAL CONDITIONS

This equipment is designed to be safe at least under the following conditions (based on the IEC

61010-1):

■ Indoor use;

■ Altitude up to 2000 m;

■ Ambient temperature 5

o

C to 40

o

C

■ Maximum relative humidity 80% for temperature up to 31

o

C decreasing linearly to 50% relative humidity

at 40

o

C;

■ Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage;

■ Other supply voltage fluctuations as stated by the manufacturer;

■ Transient overvoltages according to Installation Categories (Overvoltage Categories) II; For mains

supply the minimum and normal category is II;

■ Pollution degree 2 in accordance with IEC 60664.

7

INCUBATOR COMPONENTS

19,20 (inside)

For MCO-18AC(UV) or

when an optional UV system kit

MCO-18UVS3 is installed.

2

5

6

7

8

13

9 (inside)

Handles

1

18

3

4

Handles

10, 11

14

For MCO-18AC(UV) or

when an optional UV system kit

MCO-18UVS3 is installed.

21

16

15

12

17

Rear Left Side Rear Right Side

8

1. Outer door: Sticks to frame with magnetic seal. Door heater is installed in the outer door panel.

The door opening is reversible. Contact Sanyo representative or agent to change the door hinge from

left to right or vice versa.

2. Inner door: Made of tempered glass, however avoid excessive impact on the glass.

3. Leveling foot: Screw type for adjusting the height. Adjust the foot so that the incubator can be

level.

4. Tray: Can be pulled toward you.

5. Tray support: Can be removed by lifting the front side and pulling toward you.

6. Side support: Right and left side supports can be removed for disinfection. See page 27, 28 and

29.

7. Fan cover: Inlet of circulating air. Removable.

8. Duct: Flow path for circulating air. Removable.

9. Fan (inside the rear duct): Made from polypropylene resin. Can be disinfected by an autoclave.

10. Sample air outlet: This also functions as an internal gas outlet. Normally, cover this outlet with

the sample air outlet cap.

11. Sample air outlet cap: Always attach this cap except at the time of using of sample air outlet.

Note: Operation without sample air outlet cap may result low humidity or condensation on the outer door.

12. Connecting port for CO

2

gas pipe: When an optional component MCO-21GC (automatic CO

2

cylinder changeover system) is installed, both A and B are available. If MCO-21GC is not used, only A

is available. Refer to page 14 for gas cylinder connection. Ensure that the gas pressure is set at 0.03

MPaG (0.3 kgf/cm

2

G, 4.3 psiG). Refer to page 39 for automatic CO

2

cylinder changeover system.

13. Door switch: Detects the door opening/closing and stops the circulating fan, UV lamp* and

electromagnetic valve for CO

2

when door is open.

14. Humidifying pan: Use sterile distilled water to fill the pan. (MCO-18AC(UV) or when an optional

UV system kit MCO-18UVS3 is installed) Install the pan properly so that it can be covered with the pan

cover.

15. Remote alarm terminal: Refer to page 11.

16. Access port: When not in use, cap with the silicon caps on both outside and inside.

17. Power switch: Main switch of the incubator. Also functions as an over-current breaker. The

switch is covered by a switch cover to prevent the accidental push. To turn on or off the switch, remove

the switch cover by loosening the screw.

18. Humidifying pan cover*: Prevents UV light being exposed to the chamber. When filling the pan,

lift the front side and take out the pan. See page 30 for details.

19. UV lamp*: Sanyo UV lamp does not generate ozone. Never look at the UV light directly. For

replacement, contact Sanyo sales representative or agent.

20. Water level sensor for humidifying pan*: Detects the water level in the humidifying pan. See

page 24 for details.

21. Glow starter*: For UV lamp (model; FG-7P)

*MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed.

9

Control panel and keypad

12

13

14

151617

1

2

3

4

5

6

7

8

9

10

11

1. Digital temperature indicator (TEMPERATURE

o

C): Normally, this indicator shows the chamber

temperature. In the setting mode, it shows the set value of the chamber temperature. If the self

diagnostic function detects any abnormality, an error code will be displayed.

2. Heater lamp (HEAT): This lamp lights when the heater is energized.

3. UV indicator (UV): This lamp lights when the UV lamp is ON. The blink of this indicator

recommends the replacement of UV lamp. See page 19 for the details. MCO-18AC(UV) or when an

optional UV system kit MCO-18UVS3 is installed.

4. Door lamp (DOOR): This lamp lights when the outer door is open.

5. Water level alarm lamp (RH PAN): This lamp blinks when the water in the humidifying pan is less

than approximately 0.8 liter. MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is

installed.

6. Upper limit regulator: This regulator is used to set the upper temperature limit.

7. Over heat lamp (OVER HEAT): This lamp lights when the chamber temperature reaches the upper

limit set value.

8. CO

2

inject lamp (INJECT): This lamp lights when CO

2

gas is being injected.

9. Digital CO

2

density indicator (CO

2

%): Normally, this indicator shows the CO

2

concentration in

the chamber. In the setting mode, it indicates the set value of the CO

2

concentration. In the calibration

mode, the decimal point in the digital CO

2

density indicator blinks.

10. Set key (SET): Pressing this key to enter the setting mode, and the digits to be set will blink.

11. Calibration key (CAL): Pressing this key to enter the CO

2

calibration pre-conditioning mode.

Normally when the outer door is opened, the fan motor will stop operating. This mode allows the fan

motor to continue to circulate the chamber environment to ensure accurate CO

2

uniformity inside the

chamber. At this time, by pressing this key for approximately 5 seconds, the incubator enters the

temperature calibration input mode. And then pressing this key again, the incubator enters the CO

2

calibration input mode. See page 31 and 32 for the details. Also, used to change the UV lamp ON

period. See page 21 for the details.

Note:

This operation affects the basic performance. When calibrating the CO

2

density, contact a Sanyo sales

representative or agent.

10

12. Alarm buzzer stop key (BUZZER): Press this key to silence the buzzer when the alarm operates

and the buzzer sounds (The remote alarm is not stopped).

13. CO

2

gas supply line indicator (A/B): The lamp for the supply line currently in use lights up

provided that MCO-21GC automatic CO

2

cylinder changeover system (option) is installed.

14. CO

2

gas supply line switching key : This key to select CO

2

gas supply line is available only when

an automatic CO

2

cylinder changeover system MCO-21GC (option) is installed. When one CO

2

cylinder

is empty, the CO

2

is supplied by the other cylinder automatically.

15. Enter key (ENT): Pressing this key memorizes the set value in the controller.

16. Numerical value shift key ( ): Pressing this key in the setting mode causes the numerical value

to shift. In key lock mode, pressing this key makes key lock ON or OFF.

See page 23 for details.

17. Digit shift key (

): Pressing this key in the setting mode causes the changeable digit to shift.

Pressing this key more than 5 seconds enters key lock mode. See page 23 for the key lock.

Remote alarm terminal

The terminal of the remote alarm is installed at the rear right side of the incubator. The alarm is

outputted from this terminal. Contact capacity is DC 30 V, 2 A.

Contact output:

between COM. and N.O. between COM. and N.C.

At normal Open Close

At abnormal Close Open

Note:

The alarm is actuated when the power cord is disconnected from the outlet or the power switch is OFF.

• The remote alarm cannot be stopped by pressing the alarm buzzer stop key (BUZZER) since the

remote alarm is not conjunct with the alarm buzzer stop key (BUZZER).

Remote alarm terminal

11

INSTALLATION SITE

To operate this incubator properly and to obtain maximum performance, install the incubator in a location

with the following conditions:

WARNING

Ventilate a room air occasionally when using CO

2

gas for control. The gas density will increase in

an enclosed small room and high level of gas density can be hazardous to health. In addition, avoid

inhaling the chamber air directly when opening the door if CO

2

gas is used.

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO

2

de l’air peut s’élever et peut

être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la

porte.

■ A location not subjected to direct sunlight

Do not install the incubator under direct sunlight.

Installation in a location subjected to direct sunlight

cannot obtain the intended performance.

■ A location away from heat generating sources

Avoid installing the incubator near heat-emitting appliances such as a heater or a boiler etc. Heat can

decrease the intended performance of the incubator.

■ 5

o

C higher than the ambient temperature

The chamber temperature must be at least 5

o

C higher than the ambient temperature. For example, the

chamber temperature is set to 37

o

C, the ambient temperature must be less than 32

o

C. Keep the

ambient temperature in adequate range.

■ A location with a sturdy and level floor

Always install the incubator on a sturdy and level floor. The uneven floor or tilted installation may cause

failure or injury. Install the incubator in stable condition to avoid the vibration or noise. Unstable

condition may cause vibration or noise.

WARNING

Install the incubator on a sturdy floor. If the floor is not strong enough or the installation site is not

adequate, this may result in injury from the incubator falling or tipping over.

Select a level and sturdy floor for installation. This precaution will prevent the incubator from tipping.

Improper installation may result in water spillage or injury from the incubator tipping over.

■ A location not prone to high humidity

Install the incubator in the ambient of 80% R.H. or less humidity. Installation under high humidity may

cause current leakage or electric shock.

WARNING

Do not use the incubator outdoors. Current leakage or electric shock may result if the incubator is

exposed to rain water.

Never install the incubator in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

■ A location w

ithout flammable or corrosive gas

Never install the incubator in a flammable or corrosive location. This may cause explosion or fire or may

result in the current leakage or electric shock by the corrosion of the electrical components.

12

INSTALLATION

1. Remove the packaging materials and tapes

Remove all transportation packaging materials and tapes. Open the doors and ventilate the incubator.

If the outside panels are dirty, clean them with a neutral detergent and wipe it up with a wet cloth.

WARNING

Do not put the packing plastic bag within reach of children as suffocation may result.

2. Adjust the leveling feet

Extend the leveling feet by rotating them counterclockwise to contact them to the floor. Ensure the

incubator is level.

3. Fix the incubator

Two fixtures are attached to the rear of the frame. Fix the frame to the wall with these hooks and rope or

chain.

4. Ground (earth)

WARNING

Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is

not grounded, it is necessary to install a ground by qualified engineers.

Never ground the incubator through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

● Stacked module

When the product is used in stacked module, make sure to fix the incubator by using the stacking kit

WARNING

Do not climb onto the incubator and do not put articles on the incubator. This may cause injury by

tipping or damage to the incubator. When stacking the incubator, follow the procedure shown on page

40.

● If not used

When the incubator is not used, dispose the water in the humidifying pan and remove any moisture in the

chamber completely. Check that the chamber is completely dry before closing the doors.

● Before mov

ing the incubator

Empty the humidifying pan completely before moving the incubator. Spilled or splashed water may

cause current leakage or electric shock.

13

Connection of CO

2

gas cylinder

WARNING

Check the gas type and ensure that it is fit for the purpose. Make sure that all pipes are

connected correctly and are not liable to become disconnected. Ensure that the gas pressure is

set at the specified value. Improper connection of the gas pipe or use of incorrect gas pressure

may result in leakage of CO

2

gas. Elevated level of CO

2

gas can be hazardous to health and may

lead to asphyxiation and risk of death.

1. Use a liquefied CO

2

gas cylinder, not a siphon (dip tube) type. The CO

2

gas should be 99.5% or more

pure.

2. Install a gas pressure regulator (optional accessory MCO-100L) on the cylinder. Use a regulator

rated at 25 MPaG (250 kgf/cm

2

G, 3600 psiG) on the primary side and 0.2 MPaG (2.0 kgf/cm

2

G, 30 psiG)

on the secondary side.

3. Using the gas supply pipe provided, connect the CO

2

gas pressure regulator to the connecting port for

CO

2

gas pipe located at the rear left side of the CO

2

incubator.

Note:

If CO

2

is supplied to multiple CO

2

incubators from a single gas cylinder, a CO

2

solid will be formed in the

gas pressure regulator. The gas pressure regulator safety valve will operate, and there may an explosive

sound.

When the MCO-21GC is not mounted

Using the gas supply pipe that is provided, connect the CO

2

gas pressure regulator for the CO

2

gas

cylinder to CO

2

gas pipe connection port A on the incubator. After connecting the pipe, check to make

sure that no gas is leaking.

When the MCO-21GC is mounted

Connect a pair of CO

2

gas cylinders with CO

2

gas pressure regulators independently. The gas supply line

will be switched automatically. Connect the main cylinder to port A and the reserve cylinder to port B. After

connecting the cylinders, check to make sure that no gas is leaking.

4. Set the CO

2

pressure on the secondary side to 0.03 MPaG (0.3 kgf/cm

2

G, 4.3 psiG) (at gas injection).

Excessive pressure may cause disconnection of internal pipes inside the CO

2

incubator which will result

in leakage of CO

2

gas into the atmosphere. Elevated level of CO

2

gas can be hazardous to health

and may lead to asphyxiation and risk of death. The repair of the incubator will be necessary if the

internal pipe is disconnected.

4. Check that no gas is leaking at any point where the pipe connects with the CO

2

regulator or the CO

2

incubator.

Note:

The incubator, including the gas supply pipes and services must be examined at frequent intervals to

ensure they are safe. Ensure that items such as pipes are replaced if there is any sign of deterioration.

14

PREVENTING CONTAMINATION

To prevent contamination of the chamber, select an appropriate location for installation as well as the

complete disinfection of the chamber components.

● Avoid hot and humid location

Avoid location with high temperature and/or humidity as the presence of bacteria in the air is greater than

in normal environment.

● Avoid drafty location and location with many passers-by

Avoid locations near doors, air conditioners, fans, etc., where slight breezes can facilitate the entry of

bacteria into the chamber.

● Installation in a sterile room

To get the cultivation more efficiently, install the incubator in a sterile room.

● Use clean containers

The contamination is mainly caused by the containers such as Petri dishes or bottles stored in the

chamber. Always keep the containers clean.

● Always keep the chamber clean

The condensation may be caused on the inside of the door by spilled water form humidifying pan or

opening of outer door for long period. Wipe off the condensation completely with a sterile dry gauze.

Especially when the culture medium is spilled, clean and disinfect the chamber immediately. Refer to

page 27 “Routine maintenance” for details.

● Keep the inside panels dry

To protect the inside of the incubator from contamination, the inside panels should always be kept dry. If

water is spilled from a humidifying pan or if the door is kept open for a long period, condensation will form

on the panels, allowing germs to breed. In such a case, wipe away the water with a dry sterile gauze.

Particularly, if the medium is spilled, wipe it up immediately and sterilize the area.

● Fill the humidifying pan with sterile distilled water

Always use sterile distilled water to fill the pan. The water level alarm lamp (RH PAN) on the control

panel blinks when the water level is low. Refill the sterile distilled water to the pan when the water level

alarm lamp (RH PAN) blinks. Note that when low temperature sterile distilled water is poured, the

chamber temperature drops significantly. Clean the humidifying pan once a month.

● Do not place the incubator in the direct air flow from an air conditioning system

Air from an air conditioning system may cause condensation and lead to possible contamination.

15

PRECAUTIONS FOR CULTURES

● Do not subject to direct air flow

Do not allow the air for air conditioning to hit the incubator or door directly. Direct hit may cause

condensation or contamination.

● Allow adequate space between the cultures

When storing cultures in the chamber, keep the Petri dishes or bottles containing the cultures sufficiently

apart from each other to allow adequate air circulation. Inadequate space may result in uneven

temperature distribution and CO

2

concentration in the chamber.

● Stored materials

Never place acid or alkaline materials or materials that release corrosive gas in the chamber. Such

materials can cause failure resulting from discoloration or corrosion.

● Always shut the inner door

Shut the inner door completely, and then shut the outer door. If the inner door is not closed completely,

even if the outer door is closed, the incubator will fail to exhibit its maximum performance. And close the

doors gently. Rude closing may cause spillage of medium, incomplete closing, or damage of gasket.

● Open/close the doors gently

Ensure you close the doors gently. Robust closing may cause spillage of medium, incomplete closing,

or damage of gasket. Also, before opening the inner door, check that the UV light is OFF

(MCO-18AC(UV) or when an optional UV system kit MCO-18UVS3 is installed).

● Take care of the inside of the outer door.

It may get hot.

● Never lean or press on the glass.

Broken glass due to intentional force may cause injury.

● Alarm

Always investigate the cause and fix the alarm condition immediately when the alarm is activated. Refer

to page 25 for alarm details.

● Fix the tray supports and trays securely

Incomplete installation may cause injury or damage.

16

START-UP OF INCUBATOR

When start the test operation or the operation, follow these steps as below.

1. Install the incubator referring to “INSTALLATION” on page 13.

2. Remove all transportation packaging materials and tapes on the attachments in the chamber. Then

clean and sterilize the chamber and inner attachments. Refer to “Cleaning the chamber and inner

attachments” on page 27.

3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 30.)

4. Turn ON the power switch on the rear left side.

Note:

The humidity in the incubator chamber is adjusted to the optimum setting. To prevent condensation on

the surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan

in the bottom of chamber to recondense evaporated moisture. Condensation may occur around the

humidifying pan at the bottom of the chamber, but this does not indicate a problem.

17

SETTING THE CHAMBER TEMPERATURE AND CO

2

DENSITY

Table below shows the basic procedure for setting the chamber temperature and CO

2

density. The

upper limit alarm temperature setting is also shown in the table. Perform key operations in the sequence

indicated in the table. The example in the table is based on the assumption that the desired temperature

is 37

o

C and CO

2

density is 5%. Adjustment of the upper limit regulator should be executed after the

chamber temperature reaches the stable condition.

Note:

• The incubator is set at the factory so that the chamber temperature is 37

o

C and CO

2

density is 0%.

• When starting the incubator for the first time or after not using it for an extended period of time, allow at

least 8 hours for the chamber temperature, humidity, and CO

2

sensor to become stable after setting the

desired chamber temperature at 0% CO

2

density. Then change the setting to the desired CO

2

density.

Basic operation sequence (Example: Chamber temperature: 33

o

C, CO

2

density: 5%)

Description of operation Key operated Indication after operation

1 Turn the power switch ON. ----

The current chamber temperature is displayed

in the digital temperature indicator.

2 Press set key. SET The left digit blinks.

When pressed,

the changeable digit is shifted.

3

By pressing numerical value shift

key and digit shift key,

set the figure to 37.0.

When pressed,

the figure of settable digit changes.

4 Press enter key. ENT

Set temperature is memorized.

Left digit in the digital

CO

2

density indicator blinks.

When pressed, the changeable digit is shifted.

5

By pressing digit shift key and

numerical value shift key, set the

figure to 05.0.

When pressed,

the figure of settable digit changes.

6 Press enter key. ENT Set CO

2

density is memorized.

7

(Executed after the chamber

temperature reaches the stable

condition)

Adjust upper limit regulator so that

the alarm temp. is 1

o

C higher than

set temperature.

----

In the digital CO

2

density indicator, HI is displayed.

In the digital temperature indicator, upper limit

temp. is displayed.

The upper limit temp. can be changed

by turning upper limit regulator.

8 Press enter key. ENT

This is the end of set mode and the indicators

display current temperature and CO

2

density.

Note:

● In each set mode, if the change of the setting is not necessary, pressing set key (SET) skips to next set

mode.

● When the CO

2

density is set to 00.0, the control is OFF regardless of chamber density.

● The upper limit temperature set value will change when the regulator is turned even if the incubator is

not in set mode, because the alarm circuit is an independent circuit.

● In each set mode, the indicator returns to the current temperature and CO

2

density display mode

automatically when 90 seconds has passed without any key operation.

18

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