Thermo Fisher Scientific Locator and Locator Plus Manuale utente

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User Manual

Locator and Locator Plus

Cryogenic Storage Vessel with or without

Ultrasonic Level Monitor

Operation Manual and Parts List LT509X9 (7018141) Rev. 6

Thermo Scientific

Preface

Cryogenic Storage Vessel i

Locator Catalog Number Locator

Model Name

CY50925 w/o Monitor 8141 Locator Jr

CY50935 w/o Monitor 8143 Locator 4

CY50985 w/o Monitor 8197 Locator 6

CY50945 w/o Monitor 8145 Locator 8

CY509105 w/ Monitor 8201 Locator Jr.

CY509105CN w/ Monitor 8202 Locator Jr. China

CY509107 w/ Monitor 8246 Locator 4

CY509107CN w/ Monitor 8247 Locator 4 China

CY509113 w/ Monitor 8198 Locator 6

CY509110 w/ Monitor 8211 Locator 8

CY509110CN w/ Monitor 8212 Locator 8 China

Locator Plus Catalog Number Locator Plus

Model Name

CY50925-70 w/o Monitor 8142 Locator Jr Plus

CY50935-70 w/o Monitor 8144 Locator 4 Plus

CY50985-70 w/o Monitor 8147 Locator 6 Plus

CY50945-70 w/o Monitor 8146 Locator 8 Plus

CY509106 w/ Monitor 8237 Locator Jr Plus

CY509106CN w/ Monitor 8238 Locator Jr Plus China

CY509108 w/ Monitor 8207 Locator 4 Plus

CY509108CN w/ Monitor 8208 Locator 4 Plus China

CY509109 w/ Monitor 8209 Locator 6 Plus

CY509109CN w/ Monitor 8210 Locator 6 Plus China

CY509111 w/ Monitor 8213 Locator 8 Plus

CY509111CN w/ Monitor 8214 Locator 8 Plus China

MANUAL NUMBER LT509X9 (7018141)

6 ECNM-000831 03/16/21 Removed Warranty caution from preface page ii, added Safety Precautions in

Preface page iii, page 1-2, WEEE Compliance in page 9-1 and removed

EUT statements in page 2-1. Updated Specifications for Environmental

Conditions in page 2-1. TFI

5 41864 11/07/17 Removed CE mark and info bpg

4 40578 2/23/16 Clarified Do Not Roll warning ccs

3 -- 10/27/15 Updated symbols and labels, added cautions ccs

2 31623 5/15/15 Added caution for moving vessel in Section 3 ccs

1 31528 9/15/14 Updated conformity specs and declaration ccs

0 27564 1/31/14 Original - new PCBA design ccs

REV ECR/ECN DATE DESCRIPTION By

Thermo Scientificii Cryogenic Storage Vessel

Preface

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

CAUTION

Important Read this instruction manual. Failure to read, understand and follow instructions in this

manual may result in damage to the unit, injury to operating personnel, and poor equipment

performance. s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Warning Nitrogen gas can cause suffocation without warning. Store and use liquid nitrogen only in a

well-ventilated place. As the liquid evaporates, the resulting gas displaces the normal air in the area.

(The cloudy vapor that appears when liquid nitrogen is exposed to the air is condensed moisture, not

the gas itself. The issuing gas is invisible.) In closed areas, excessive amounts of nitrogen gas reduces the

concentration of oxygen and can result in asphyxiation. Because nitrogen gas is colorless, odorless, and

tasteless, it cannot be detected by the human senses. Breathing an atmosphere that contains less than

19.5% oxygen can cause dizziness and quickly result in unconsciousness and death. Therefore, the use

of oxygen monitoring equipment is strongly recommended. s

Warning Contact of liquid nitrogen with the skin or eyes may cause serious (freezing) injury. s

Warning Do not roll. Always keep liquid nitrogen vessel in an upright position. Exercise caution to

prevent spilling and splashing. s

Material in this manual is for information purposes only. The contents and the product it describes are subject to

change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this

manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to

the use of this manual.

Intended Use:

The Thermo Fisher Scientific cryogenic storage vessels are intended to store biological samples at cryogenic

temperatures. These products are comprised of a vessel and a lid, with or without a level monitor.

Unintended Use:

These products are not intended for use within the patient environment.

When this manual is translated into other languages, the US English version is binding.

Thermo Scientific Cryogenic Storage Vessel iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this

symbol.

Extreme temperature hazards. Only qualified persons should perform procedures associated with this

symbol.

Potential biological hazards. Proper protective equipment and procedures must be used.

This symbol when used alone indicates important operating instructions which reduce the risk of

injury or poor performance of the unit.

CAUTION: This symbol, in the context of a CAUTION, indicates a potentially hazardous situation

which if not avoided could result in minor to moderate injury or damage to the equipment. s

CAUTION: This indicates a situation which may result in property damage. s

Before installing, using or maintaining this product, please be sure to read the manual and product

warning labels carefully. Failure to follow these instructions may cause the product to malfunction,

which could result in injury or damage.

WARNING: Unauthorized repair of your freezer will invalidate your warranty. Contact Technical

Service at 1-800-438-4851 for additional information. s

Thermo Scientificiv Cryogenic Storage Vessel

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct

1-800-438-4851 Toll Free, U.S. and Canada

1-877-213-8051 FAX

http://www.thermofisher.com Internet Worldwide Web Home Page

service.led.marietta@thermofisher.com Tech Support Email Address

Certified Service Web Pagewww.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We canSales Support

take your order and provide delivery information on major equipment items or make

arrangements to have your local sales representative contact you. Our products are listed on the

Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation orService Support

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or

provide you with on-site service. We can also provide you with a quotation on our Extended

Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your

applications. If you are experiencing technical problems, working together, we will help you

locate the problem and, chances are, correct it yourself...over the telephone without a service

call.

When more extensive service is necessary, we will assist you with direct factory trained

technicians or a qualified service organization for on-the-spot repair. If your service need is

covered by the warranty, we will arrange for the unit to be repaired at our expense and to your

satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you

Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by

telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific (Asheville) LLC

401 Millcreek Road, Box 649

Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Cryogenic Storage Vessel vThermo Scientific

Table of Contents

Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Please Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

General Usage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Filling Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Measuring Liquid Nitrogen Quantity . . . . . . . . . . . . . . . . . . . . . . . .4-2

Storing Samples in Vapor Phase . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

General Cleaning Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Liquid Nitrogen Level Monitor Operation . . . . . . . . . . . . . . . . . . . . . . . .6-1

Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Remote Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Alarm Contact Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Microprocessor Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4

Principles of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4

Level Monitor Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Safety Feature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Audible and Visual Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

LN2 Level Monitor Troubleshooting and Parts List . . . . . . . . . . . . . . .7-1

Ordering Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2

Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

WEEE Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Section 1

Section 2

Section 3

Section 4

Section 5

Section 6

Section 7

Section 8

Section 9

vi Cryogenic Storage Vessel Thermo Scientific

Table of Contents

Cryogenic Storage Vessel 1-1Thermo Scientific

Please Read

Section 1 Safety Information

This manual contains important operating and safety information.

The user must carefully read and understand the contents of this manual

prior to the use of this equipment.

Your Thermo Scientific cryogenic vessel has been designed with function,

reliability, and safety in mind. It is the user’s responsibility to install it in

conformance with local electrical codes. For safe operation, please pay

attention to the warnings and cautions throughout the manual.

All cryopreservation vessels, regardless of who manufactures them, use a

vacuum to provide the super insulative properties needed to keep nitrogen

in a liquid form. They are very similar, in fact, to the Thermos® vessels you

may have used to store coffee, soup or milk.

Remember how fragile they were? Since no vacuum is perfect or will last

forever, we suggest that you monitor the consumption of liquid nitrogen

used by your vessel on a regular basis, i.e. every 1-2 days. By monitoring,

you may be able to anticipate subtle changes in consumption and possibly

react to problems before they arise. You may opt to use a level monitor for

this purpose (see page 6-1 for level monitor operation.).

Also, if your samples are super critical, one-of-a-kind, or irreplaceable,

consider allocating your samples to 2 separate vessels. Remember the old

adage about placing all your eggs into one basket….

Do not use this product for anything other than its intended usage.

Use of the equipment in a manner not specified by the manufacturer may

impair the protection provided by the equipment.

General Usage

1-2 Cryogenic Storage Vessel Thermo Scientific

Section 1

Safety Information

• This unit is intended to be used by trained personnel in a

well-ventilated environment.

• Liquid nitrogen is extremely cold; it boils at -196°C.

• Follow all necessary precautions and warnings relevant to applicable

hazards such as frostbite, contact burn, asphyxiation, etc. in cryogenic

area.

• To avoid injury due to frostbite or ruptured vials, use extreme care

whenever handling liquid nitrogen, liquid nitrogen storage or transfer

vessels, or any objects which have come in contact with liquid

nitrogen.

• Leave no areas of skin exposed.

• Wear appropriate PPE where necessary; face shield, cryogenic gloves,

cryogenic apron.

• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas

from escaping.

• Always handle ampules with tongs. They may explode when removed

from the vessel.

• Use extreme care to prevent spilling and splashing liquid nitrogen

during transfer and removal of storage contents and holders.

• Immediately remove any clothing or safety attire on which liquid

nitrogen has been spilled or splashed.

• Get immediate medical attention for any frostbite injuries due to

liquid nitrogen.

Use this product only in the way described in the product literature•

and in this manual. Before using it, verify that this product is suitable

for its intended use. If the equipment is used in a manner not

specified by the manufacturer, the protection provided by the

equipment may be impaired.

Warnings

• Only use the power supply provided by the unit.

• Never overfill liquid nitrogen vessels. Liquid nitrogen level should

never be above the tops of the racks inside the vessel. The excess

weight may crack the neck and result in vacuum failure.

• When inserting or removing racks, be careful not to come in contact

with the neck tube area of the vessel. Remove or insert racks slowly

in a vertical manner. Scratches on the neck tube area can cause

premature vacuum failure.

• Do not tamper with or remove vacuum port (covered by a black

plastic cap on side of vessel); this will void warranty.

• Do not spill liquid nitrogen on vacuum port - this can cause vacuum

failure.

• Always keep liquid nitrogen vessel in an upright position.

• Do not use this Locator vessel for transport.

Cryogenic Storage Vessel 1-3Thermo Scientific

Section 1

Safety Information

Cautions

1-4 Cryogenic Storage Vessel Thermo Scientific

Section 1

Safety Information

Cryogenic Storage Vessel 2-1Thermo Scientific

Operating temperature: -29°C to +60°C

10% to 95% relative humidity, non-condensing.

Altitude limit: 2,000 meters.

Storage: -29°C to +60°C; 10% to 95% relative humidity.

Intended for indoor use only.

Inputs 100-240 VDC, 0.08 - 0.04 amps, single phase

Frequency . . . . . . . . . . . . . . . . . . . . . . .47 - 63 Hz

Output . . . . . .15 VDC (±10%), 0.2 amps, 3.0 watts

Locator 4 Locator 4 Plus Locator 6 Locator 6 Plus Locator 8 Locator 8 Plus Locator JR Locator JR Plus

Height

(including lid)1

37.5”

(95.2 cm) 39.5” (100.3 cm) 39.5”

(100.3 cm) 39.5” (100.3 cm) 37.5”

(95.2 cm) 39.5” (100.3 cm) 26.5”

(67.3 cm) 28.9” (73.4 cm)

Diameter 22”

(55.9 cm) 22” (55.9 cm) 26” (66 cm) 26” (66 cm) 22”

(55.9 cm) 22” (55.9 cm) 22” (55.9 cm) 22” (55.9 cm)

LN2 Capacity,

Liquid Phase

Storage

111 L 121 L 184 L 184 L 111 L 121 L 60 L 71 L

Static LN2

Evaporation

rate - L/Day3

0.99 0.99 0.99 0.99 0.60 0.60 0.85 0.85

Liquid Phase

Capacity (2mL

Vial2actually

submerged in

liquid nitrogen)

3600 4000 6000 6000 1800 2000 1600 2000

1The liquid nitrogen level monitor will add 1 inch to these heights. The transportation cart will add 4 inches to these heights.

2 Using Nalgene System 100 cryoboxes with a 10 x 10 vial configuration (Nalgene System 100 vial or equivalent are needed).

3Actual working time may vary due to current atmospheric conditions, container history, manufacturing tolerances, and any individual patterns

of use.

Environmental

Conditions

Electrical

(cover with monitors only)

Section 2 General Specifications

2-2 Cryogenic Storage Vessel Thermo Scientific

Section 2

General Specifications

Cryogenic Storage Vessel 3-1Thermo Scientific

Section 3 Unpacking

Before using your new Locator or Locator Plus vessel, carefully inspect the

vessel prior to use. Check for signs of damage which may have occurred in

shipment. It is advisable to fill (see filling instructions) all new units with

liquid nitrogen and watch liquid nitrogen loss rate for a few days. If there

are any problems, call Customer Service as soon as possible.

The warranty registration card enclosed with the vessel must be completed

and returned to the factory within 30 days to make warranty effective.

This information must include the serial number, which is located on the

label on the vessel.

Note The most prevalent cause of failure of liquid nitrogen storage vessels

is mechanical. The vessel necktube supports the full weight of the inner

shell and all the liquid nitrogen it contains. A side blow to the vessel causes

the inner shell to swing in a pendulum-like motion causing the necktube

to be damaged. Any storage vessel which has been in an accident, has been

dropped, or lowered to hit on its side, will tend to fail more rapidly than

one that has not.

Caution Exercise caution when moving your Locator or Locator Plus

vessel. Locator and Locator Plus cryobiological storage systems are not

transportation vessels. Transport carts are designed for mobility within the

lab, or lab to lab only. Moving full vessels long distances, over cracks in

floor, thresholds, on inclined ramps or in elevators can cause premature

vacuum failure. Avoid using excessive force when moving vessel; this can

cause tipping, resulting in damage and/or spilling of liquid nitrogen. s

Note If samples must be transported under cryogenic conditions, consider

Arctic Express line of Dry Shippers available from Thermo Scientific.

3-2 Cryogenic Storage Vessel Thermo Scientific

Section 3

Unpacking

Cryogenic Storage Vessel 4-1Thermo Scientific

Section 4 Operation

Caution Never overfill your Locator or Locator Plus vessel with liquid

nitrogen. The liquid nitrogen level in your tank (with racks inserted)

should never be above 20 inches (50 cm) for Locator 4 and Locator 8, 10

inches (25 cm) for Locator Jr., 22 inches (55.8 cm) for Locator 4, 6, and 8

Plus and 12 inches for Locator Jr. Plus. Filling the tank up to or above the

bottom of the necktube may cause immediate or premature vacuum failure

to occur. s

Caution When inserting or removing racks, be careful not to come in

contact with the necktube area of the vessel. Remove or insert racks slowly

in a vertical manner. Scratches on the necktube area can cause premature

vacuum failure. s

Caution Do not spill liquid nitrogen on vacuum port (covered by a black

plastic cap on side of vessel). This can cause vacuum failure. s

To avoid damage to your Locator or Locator Plus cryogenic storage vessel

which may result in premature vacuum loss, it is important that the

following procedure be used when adding liquid nitrogen to a warm vessel.

1. Add only a small amount of liquid nitrogen (5-10 liters) to a new or

warm vessel.

2. Allow this small amount of liquid nitrogen to sit in the covered vessel

for a minimum of 2 hours. This will limit stress caused by the sudden

temperature change associated with adding liquid nitrogen to a warm

vessel.

3. Add an additional 15 liters of liquid nitrogen to the vessel.

4. Allow vessel to sit for 48 hours and monitor liquid nitrogen

consumption with a wooden yarsdstick, optional dip measurer (part

#180143) or Level Monitor.

5. Fill Locator or Locator Plus as indicated (see Caution in this section).

Allow for displacement of liquid nitrogen when racks and boxes are

inserted.

6. Insert and remove racks slowly. Allow liquid nitrogen to run out of

boxes and off racks.

Filling Instructions

4-2 Cryogenic Storage Vessel Thermo Scientific

Section 4

Operation

1. Use a wooden yardstick or optional dip measurer (part #180143) to

measure liquid nitrogen level. NEVER use a hollow tube or plastic

dipstick to measure the liquid nitrogen level.

2. Level will be indicated by the frostline which develops when dipstick is

removed.

3. Level Monitor can be used for constant measurements. See Section 6.

1. Remove the 2 bottom-most cryoboxes from each rack.

2. Measure the height of 2 cryoboxes stacked on top of each other.

3. Fill vessel with liquid nitrogen to the height obtained from Step 2,

using a yardstick to measure liquid level in vessel.

4. Place racks into vessel without the 2 bottom-most cryoboxes. Samples

are now above the liquid nitrogen and are stored in the vapor phase.

Important A level monitor should always be used when you store your

samples in the vapor phase because of the already low levels of liquid

nitrogen. Refer to Section 5 for correct level monitor for your system. s

Wipe exterior surfaces with lightly dampened cloth containing mild soap

solution.

Storing Samples in

Vapor Phase

General Cleaning

Instructions

Measuring Liquid

Nitrogen Quantity

Cryogenic Storage Vessel 5-1Thermo Scientific

Section 5 Replacement Parts

Locator and Locator Plus Covers w/o Monitor

Type Standard Covers Covers and Rack Kits (for units with

lids made before 3/2010)

Locator Jr. CV509X51 (321746) 1950514

Locator Jr. Plus CV509X51 (321746) 1950515

Locator 4 CV509X51 (321746) 1950516

Locator 4 Plus CV509X51 (321746) 1950517

Locator 6 CV509X50 (321651) 1950513

Locator 6 Plus CV509X50 (321651) 1950513

Locator 8 CV509X23 (317227) CV509X23 (317227)

Locator 8 Plus CV509X23 (317227) CV509X23 (317227)

5-2 Cryogenic Storage Vessel Thermo Scientific

Section 5

Replacement Parts

Catalog # Rack Description

HR509X15A (317050) For Locator Jr (8141, 8201, 8202) with 4 - 2 inch boxes

HR509X2A (317161) For Locator 4 (8143, 8246, 8247) with 9 - 2 inch boxes

HR509X19A (315903) For Locator Jr (8141, 8201, 8202) with 2 - 4 inch boxes

HR509X20A (315905) For Locator 4 (8143, 8246, 8247) with 5 - 4 inch boxes

HR509X15A-70 (317148) For Locator Jr Plus (8142, 8237, 8238) with 5 - 2 inch boxes

HR509X2A-70 (317175) For Locator 4 Plus (8144, 8207, 8208) with 10 - 2 inch boxes

HR509X31A-70 (317192) For Locator 6 and 6 Plus (8147, 8197, 8198, 8209, 8210) with 10 - 2 inch boxes

HR509X1A (317205) For Locator 8 (8211, 8212, 8145) with 9 - 2 inch boxes (25 cell)

HR509X1A-70 (317217) For Locator 8 Plus (8146, 8213, 8214) with 10 - 2 inch boxes (25 cell)

HR509X19A-70 (315904) For Locator Jr. Plus (8142, 8237, 8238) with 2 - 4 inch boxes + 1 - 2 inch box

HR509X20A-70 (315906) For Locator 4 Plus (8144, 8207, 8208) with 5 - 4 inch boxes + 1 - 2 inch box

HR509X32A-70 (315907) For Locator 6 and 6 Plus (8147, 8197, 8198, 8209, 8210) with 5 - 4 inch boxes + 1 - 2 inch box

Section 6 Liquid Nitrogen Level

Monitor Operation

The liquid nitrogen (LN2) level monitor provides a constant indication of

the LN2level and notifies you to low level conditions through audible and

visual alarms. It alerts you when your vessel needs filling. The level

monitor is shipped mounted directly to the appropriate Locator or Locator

Plus cover. It can be wired to a remote alarm system to alert you to

problems when you are not in your laboratory. This equipment was

intended to be operated with an external power supply provided by the

manufacturer. It is designed for use with Thermo Scientific LN2vessels

only.

Caution Use this product for its intended usage only. s

Warning Potential electrical hazards. Only qualified persons should

perform procedures associated with this symbol. s

Note If the level monitor was purchased as part of a Locator or Locator

Plus system, skip to Step 3. If it is a retrofit for an existing Locator or

Locator Plus, begin with Step 1. s

1. Remove the level monitor and Locator or Locator Plus cover from box.

2. Remove present cover from your Locator or Locator Plus and replace

with your new level monitor and cover.

3. Check the electrical specifications on the power supply for electrical

data and plug in to appropriately grounded receptacle.

Cryogenic Storage Vessel 6-1Thermo Scientific

Installation

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