Thermo Fisher Scientific Ion Max and Ion Max-S API Source Manuale del proprietario

For Research Use Only

Not for use in Diagnostic

Procedures

97055-97044 Revision D November 2008

Ion Max and Ion

Max-S API Source

Hardware Manual

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The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-

free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might

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Release history: Revision A released 2005, Revision B released 2006, Revision C released January 2008,

Revision D released November 2008.

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug

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This product is required to comply with the European Union’s Waste Electrical & Electronic

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Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each

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détection des substances sujettes à la directive RoHS sont disponibles sur www.thermo.com/

WEEERoHS.

CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Elektroschock: In diesem Gerät werden

Hochspannungen verwendet, die

Verletzungen verursachen können.

Vor Wartungsarbeiten muß das Gerät

abgeschaltet und vom Netz getrennt

werden. Betreiben Sie Wartungsarbeiten

nicht mit abgenommenem Deckel. Nehmen

Sie die Schutzabdeckung von Leiterplatten

nicht ab.

Choc électrique : L’instrument utilise des

tensions capables d’infliger des blessures

corprelles. L’instrument doit être arrêté

et débranché de la source de courant

avant tout intervention. Ne pas utiliser

l’instrument sans son couvercle. Ne pas

elensver les étuis protecteurs des cartes de

circuits imprimés.

Descarga eléctrica: Este instrumento

utiliza altas tensiones, capaces de

producir lesiones personales. Antes de

dar servicio de mantenimiento al

instrumento, éste debera apagarse y

desconectarse de la línea de alimentacion

eléctrica. No opere el instrumento sin sus

cubiertas exteriores quitadas. No remueva

las cubiertas protectoras de las tarjetas

de circuito impreso.

Shock da folgorazione. L’apparecchio

è alimentato da corrente ad alta tensione

che puo provocare lesioni fisiche.

Prima di effettuare qualsiasi intervento

di manutenzione occorre spegnere ed

isolare l’apparecchio dalla linea elettrica.

Non attivare lo strumento senza lo

schermo superiore. Non togliere i coperchi

a protezione dalle schede di circuito

stampato (PCB).

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals.

Use approved containers and proper

procedures to dispose waste oil.

Chemikalien: Dieses Gerät kann

gefährliche Chemikalien enthalten.

Tragen Sie Schutzhandschuhe beim

Umgang mit toxischen, karzinogenen,

mutagenen oder ätzenden/reizenden

Chemikalien. Entsorgen Sie verbrauchtes

Öl entsprechend den Vorschriften in den

vorgeschriebenen Behältern.

Chimique : Des produits chemiques

dangereux peuven se trouver dans

l’instrument. Proted dos gants pour

manipuler tous produits chemiques

toxiques, cancérigènes, mutagènes, ou

corrosifs/irritants. Utiliser des récipients

et des procédures homologuées pour se

débarrasser des déchets d’huile.

Química: El instrumento puede contener

productos quimicos peligrosos. Utilice

guantes al manejar productos quimicos

tóxicos, carcinogenos, mutagenos o

corrosivos/irritantes. Utilice recipientes y

procedimientos aprobados para

deshacerse del aceite usado.

Prodotti chimici. Possibile presenza di

sostanze chimiche pericolose

nell’apparecchio. Indossare dei guanti per

maneggiare prodotti chimici tossici,

cancerogeni, mutageni, o

corrosivi/irritanti. Utilizzare contenitori

aprovo e seguire la procedura indicata per

lo smaltimento dei residui di olio.

Heat: Before servicing the instrument,

allow any heated components to cool.

Hitze: Warten Sie erhitzte Komponenten

erst nachdem diese sich abgekühlt haben.

Haute Temperature : Permettre aux

composants chauffés de refroidir avant

tout intervention.

Altas temperaturas: Permita que lop

componentes se enfríen, ante de efectuar

servicio de mantenimiento.

Calore. Attendere che i componenti

riscaldati si raffreddino prima di

effetturare l’intervento di manutenzione.

Fire: Use care when operating the system

in the presence of flammable gases.

Feuer: Beachten Sie die einschlägigen

VorsichtsmaBnahmen, wenn Sie das

System in Gegenwart von entzündbaren

Gasen betreiben.

Incendie : Agir avec précaution lors de

l’utilisation du système en présence de

gaz inflammables.

Fuego: Tenga cuidado al operar el

sistema en presencia de gases

inflamables.

Incendio. Adottare le dovute precauzioni

quando si usa il sistema in presenza di gas

infiammabili.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

Verletzungsgefahr der Augen:

Verspritzte Chemikalien oder kleine

Partikel können Augenverletzungen

verursachen. Tragen Sie beim Umgang

mit Chemikalien oder bei der Wartung

des Gerätes eine Schutzbrille.

Danger pour les yeux : Dex projections

chimiques, liquides, ou solides peuvent

être dangereuses pour les yeux. Porter des

lunettes de protection lors de toute

manipulationde produit chimique ou pour

toute intervention sur l’instrument.

Peligro par los ojos: Las salicaduras de

productos químicos o particulas que

salten bruscamente pueden causar

lesiones en los ojos. Utilice anteojos

protectores al mnipular productos

químicos o al darle servicio de

mantenimiento al instrumento.

Pericolo per la vista. Gli schizzi di

prodotti chimici o delle particelle presenti

nell’aria potrebbero causare danni alla

vista. Indossare occhiali protettivi quando

si maneggiano prodotti chimici o si

effettuano interventi di manutenzione

sull’apparecchio.

General Hazard: A hazard is present

that is not included in the above

categories. Also, this symbol appears

on the instrument to refer the user to

instructions in this manual.

Allgemeine Gefahr: Es besteht eine

weitere Gefahr, die nicht in den

vorstehenden Kategorien beschrieben ist.

Dieses Symbol wird im Handbuch

auBerdem dazu verwendet, um den

Benutzer auf Anweisungen hinzuweisen.

Danger général : Indique la présence

d;un risque n’appartenant pas aux

catégories citées plus haut. Ce symbole

figure également sur l’instrument pour

renvoyer l’utilisateur aux instructions du

présent manuel.

Peligro general: Significa que existe un

peligro no incluido en las categorias

anteriores. Este simbolo también se utiliza

en el instrumento par referir al usuario

a las instrucciones contenidas en este

manual.

Pericolo generico. Pericolo non

compreso tra le precedenti categorie.

Questo simbolo è utilizzato inoltre

sull’apparecchio per segnalare all’utente

di consultare le istruzioni descritte nel

presente manuale.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Wenn Sie sich über die Sicherheit

eines Verfahrens im unklaren sind,

setzen Sie sich, bevor Sie fortfahren,

mit Ihrer lokalen technischen

Unterstützungsorganisation für Thermo

Fisher Scientific San Jose Produkte in

Verbindung.

Si la sûreté d’un procédure est incertaine,

avant de continuer, contacter le plus

proche Service Clientèle pour les produits

de Thermo Fisher Scientific San Jose.

Cuando la certidumbre acerca de un

procedimiento sea dudosa, antes de

proseguir, pongase en contacto con la

Oficina de Asistencia Tecnica local para

los productos de Thermo Fisher Scientific

San Jose.

Quando e in dubbio la misura di sicurezza

per una procedura, prima di continuare,

si prega di mettersi in contatto con il

Servizio di Assistenza Tecnica locale per i

prodotti di Thermo Fisher Scientific San

Jose.

CAUTION Symbol CAUTION

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals.

Use approved containers and proper

procedures to dispose waste oil.

Heat: Before servicing the instrument,

allow any heated components to cool.

Fire: Use care when operating the system

in the presence of flammable gases.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

General Hazard: A hazard is present

that is not included in the above

categories. Also, this symbol appears

on the instrument to refer the user to

instructions in this manual.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Thermo Scientific Ion Max and Ion Max-S API Source Hardware Manual vii

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Regulatory Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .x

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .x

Chapter 1 Ion Max and Ion Max-S API Source Housings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

Functional Description of the API Source Housing . . . . . . . . . . . . . . . . . . . . . . .3

Adjusting the Probe Position on the Ion Max API Source Housing . . . . . . . . . . .5

Removing the API Source Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6

Installing the API Source Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

API Source Housing Drain. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Chapter 2 Electrospray Ionization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Theory of Electrospray Ionization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Functional Description of the ESI Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14

Removing the ESI Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

Installing the ESI Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

Maintaining the ESI Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

Trimming the ESI Sample Tube. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

Disassembling the ESI Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

Cleaning or Replacing the ESI Probe Components. . . . . . . . . . . . . . . . . . . . .25

Assembling the ESI Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

Installing a Fused-Silica Sample Tube and PEEK Safety Sleeve . . . . . . . . . . . . .28

Installing an Optional Metal Needle Sample Tube. . . . . . . . . . . . . . . . . . . . . . .33

Replaceable Parts for the ESI Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Chapter 3 Heated-Electrospray Ionization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Introduction to the HESI-II Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

Functional Description of the HESI-II Probe. . . . . . . . . . . . . . . . . . . . . . . . . . .39

Removing the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

Installing the HESI-II Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

Maintaining the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

Flushing the Sample Transfer Line and Sample Tube. . . . . . . . . . . . . . . . . . .51

Replacing the Needle Insert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52

Replaceable Parts for the HESI-II Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55

Contents

viii Ion Max and Ion Max-S API Source Hardware Manual Thermo Scientific

Chapter 4 Atmospheric Pressure Chemical Ionization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Theory of Atmospheric Pressure Chemical Ionization . . . . . . . . . . . . . . . . . . . .58

Functional Description of the APCI Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . .60

Removing the APCI Probe and the Corona Needle . . . . . . . . . . . . . . . . . . . . . .62

Installing the APCI Probe and the Corona Needle . . . . . . . . . . . . . . . . . . . . . . .64

Maintaining the APCI Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68

Removing the APCI Nozzle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68

Cleaning the APCI Nozzle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69

Removing the APCI Sample Tube from the APCI Nozzle . . . . . . . . . . . . . . .70

Installing the APCI Sample Tube . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72

Reassembling the APCI Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73

Replaceable Parts for the APCI Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

Thermo Scientific Ion Max and Ion Max-S API Source Hardware Manual ix

P

Preface

This manual describes how to install the API source housing and how to install and

maintain the ESI, HESI-II, and APCI probes.

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and

other special notices appear in boxes.

Safety and special notices include the following:

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to

ensure full compliance with applicable domestic and international regulations. When the

system is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and

safety standards. For more information, refer to the Regulatory Compliance sheet in the

hardware manual provided with your Thermo Scientific mass spectrometer.

CAUTION Highlights hazards to humans, property, or the environment. Each

CAUTION notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or may contain information that is critical for optimal

performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

CAUTION Read and understand the various precautionary notes, signs, and symbols

contained inside this manual pertaining to the safe use and operation of this product

before using the device.

Preface

xIon Max and Ion Max-S API Source Hardware Manual Thermo Scientific

Safety Precautions

Observe the following safety precautions when you operate the instrument or perform

maintenance on the API probes:

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need.

YTo contact Technical Support

Find software updates and utilities to download at mssupport.thermo.com.

CAUTION Do not perform any servicing other than that contained in the manual. To

avoid personal injury or damage to the instrument, do not perform any servicing other

than that contained in the Ion Max and Ion Max-S API Source Hardware Manual or

related manuals unless you are qualified to do so.

CAUTION Install a safety sleeve over the ESI fused-silica sample tube. You could

receive an electrical shock if the fused-silica capillary tube breaks during ESI operation.

Therefore, for your safety and in compliance with international safety standards, you

must cover the fused-silica capillary tube with the PEEK safety sleeve (P/N 00301-22806)

and associated PEEK ferrules (P/N 00101-18119) provided in the Safety Sleeve Kit

(P/N 70005-62015) before you operate the instrument.

CAUTION Respect heated zones. Treat heated zones with respect. The ion transfer

capillary, the APCI vaporizer, and the HESI-II probe might be very hot and might cause

severe burns if touched. Allow heated components to cool before you service them.

CAUTION Place the MS detector in Standby mode (or Off) before you open the

atmospheric pressure ionization (API) source. The presence of atmospheric oxygen in

the API source when the MS detector is on could be unsafe. The MS detector

automatically goes into Standby mode when you open remove API source housing, open

the housing door (Ion Max), or unplug the vaporizer cable from the housing or the APCI

or HESI-II probe; however, it is best to take this added precaution.

CAUTION Handle the corona discharge needle with care. The corona discharge needle

is very sharp and can puncture your skin.

Phone 800-532-4752

Fax 561-688-8736

E-mail us.techsupport.analyze@thermofisher.com

Knowledge base www.thermokb.com

Preface

Thermo Scientific Ion Max and Ion Max-S API Source Hardware Manual xi

YTo contact Customer Service for ordering information

YTo copy manuals from the Internet

Go to mssupport.thermo.com and click Customer Manuals in the left margin of the window.

YTo suggest changes to documentation or to Help

• Fill out a reader survey online at www.thermo.com/lcms-techpubs.

• Send an e-mail message to the Technical Publications Editor at

techpubs-lcms@thermofisher.com.

Phone 800-532-4752

Fax 561-688-8731

E-mail us.customer-support.analyze@thermofisher.com

Web site www.thermo.com/ms

Thermo Scientific Ion Max and Ion Max-S API Source Hardware Manual 1

1

Ion Max and Ion Max-S API Source Housings

This chapter describes the Ion Max and Ion Max-S atmospheric pressure ionization (API) source

housings (see Figure 1). The Ion Max and Ion Max-S API source housings provide the same

functionality with the following exceptions: the Ion Max has an adjustable probe mount and a

front door with a view window. The adjustable probe mount allows you to adjust the XY position

of the probe and the front door makes it easier to install additional options. If your system uses the

Ion Max-S API source housing, you can only adjust the probe depth.

Figure 1. Ion Max-S (left) and Ion Max (right) API sources

Contents

•Introduction

•Functional Description of the API Source Housing

•Adjusting the Probe Position on the Ion Max API Source Housing

•Removing the API Source Housing

•Installing the API Source Housing

•API Source Housing Drain

1 Ion Max and Ion Max-S API Source Housings

Introduction

2Ion Max and Ion Max-S API Source Hardware Manual Thermo Scientific

Introduction

All mass spectrometers require an ionization source (ion source) to generate ions. The popularity of

LC/MS techniques in mass spectrometry has made atmospheric pressure ionization (API) sources

the industry standard. The specific process used to ionize the sample is referred to as the ionization

mode. The Ion Max™ and Ion Max-S™ API sources can be configured to operate in any of several

API modes, including electrospray ionization (ESI), heated-electrospray ionization (H-ESI),

atmospheric pressure chemical ionization (APCI), and atmospheric pressure photo-ionization

(APPI). The ion guides transmit the ions produced in the API source into the mass analyzer, where

they are separated according to their mass-to-charge ratios.

Tab le 1 lists which API source housing is provided with your mass spectrometer.

With both of the Ion Max API source housings, you can switch between ionization modes without

using specialized tools. Pressure in the ion source housing is kept at atmospheric levels, which

reduces the chemical noise that nebulized gases can cause when they are not properly evacuated

from the ion source. During operation, the external surface of the ion source housing can become

hot. The ventilation holes in the ion source housing aid cooling when the system is placed in

Standby mode. Allow the system to cool for a minimum of 20 minutes before touching the ion

source housing.

The probe mounting angle is fixed at the optimum angle for signal intensity and ion source

robustness. You can use the probe adjustment markers (A, B, C, and D depth markers) to record

the probe position used during ionization optimization. View ports on the ion source housing

allow you to view the probe while positioning it. These ports also help you add accessories.

Corrosion of the ion source caused by liquid leaks or poor drainage is prevented by these features:

• The drain size and angle allow eluants to flow directly from the probe into the drain when

auxiliary gases are off. For liquids that do not enter the drain directly, the floor of the ion

source interior is specially sloped to enable maximum drainage of collected eluants.

• The zero dead volume (ZDV) grounding unions that connect the solvent flow from the liquid

chromatograph or the syringe pump to the ESI or HESI-II probe sample inlets are offset to

prevent liquid leaks from dripping into the source housing.

Both of the API source housings incorporate a universal mounting platform and interface for use

with ESI, H-ESI, APCI, and APPI ionization sources. For information on the analysis of ions

produced by the API sources, refer to the hardware manual that comes with your mass

spectrometer.

Table 1. API source housings provided with Thermo Scientific mass spectrometers

TSQ Series LTQ Series API source housing

TSQ Quantum Access LCQ Fleet

LXQ

Ion Max-S

TSQ Quantum Ultra

TSQ Vantage

LTQ and LTQ XL

LTQ Velos

Ion Max

CAUTION Avoid burns. Avoid touching the ion source housing when the mass spectrometer is

in operation. In the APCI and H-ESI modes, the external surface of the housing can become

hot enough to burn your skin.

1 Ion Max and Ion Max-S API Source Housings

Functional Description of the API Source Housing

Thermo Scientific Ion Max and Ion Max-S API Source Hardware Manual 3

Functional Description of the API Source Housing

The Ion Max and Ion Max-S API source housings include two nitrogen inlets, a drain port for

waste liquid, a vaporizer cable for the APCI or HESI-II probe heaters, and an 8 kV cable for the

electrospray needle (ESI) or corona discharge needle (APCI).

The Ion Max housing also includes a door to access the API probe and a movable probe port for

precise probe positioning. You use the micrometer in front of the probe port to move the probe

nozzle closer to or farther away from the ion source interface.

Figure 2 shows the vaporizer cable, 8 kV cable, and nitrogen lines on the top portion of the Ion

Max housing. In the H-ESI and APCI modes, the vaporizer cable provides power to the probe

heater. In the ESI mode, the vaporizer cable remains connected to the interlock socket. In the ESI

and H-ESI modes, the 8 kV cable supplies current to the ESI needle. In the APCI mode, the 8 kV

cable supplies current to the APCI corona needle attached to the source housing. For all three

ionization modes, the auxiliary and sheath gas lines supply nitrogen flow to the probes.

Figure 2. Ion Max housing viewed from the top

A

S

8 kV cable

socket with

cover

Micrometer for adjusting the

Y-axis probe mount position

Door

Vaporizer cable

Interlock socket

8 kV cable

Auxiliary gas line

Sheath gas line

Knob for

adjusting the

X-axis probe

mount

position

1 Ion Max and Ion Max-S API Source Housings

Functional Description of the API Source Housing

4Ion Max and Ion Max-S API Source Hardware Manual Thermo Scientific

The API source housings have high voltage safety interlock switches that turn off the following

voltages:

• ESI needle voltage (or APCI corona discharge voltage)

• All API source and lens voltages, including the ion transfer capillary offset voltage

• The voltages on the ion guides

The following actions turn off these voltages:

• Removing the API source housing from the front of the mass spectrometer

• Removing the probe guide pin from the interlock block

• Disconnecting the vaporizer cable from the interlock socket on the API source housing (ESI

mode) or from the probe (H-ESI or APCI mode)

• Opening the Ion Max housing door

1 Ion Max and Ion Max-S API Source Housings

Adjusting the Probe Position on the Ion Max API Source Housing

Thermo Scientific Ion Max and Ion Max-S API Source Hardware Manual 5

Adjusting the Probe Position on the Ion Max API Source Housing

If you have the Ion Max API source housing, to maximize sensitivity, you can adjust the

side-to-side and front-to-back probe position by a few millimeters. Typically, you adjust the probe

position while optimizing the tune parameters for your analytes. For information on using the tune

software for your mass spectrometer, refer to the getting started guide for your mass spectrometer.

YTo adjust the probe position

• Using the micrometer on the front of the Ion Max source housing, adjust the front-to-back

probe position (see Figure 3).

• Using the knurled nut on the left side and the +1 to -1 markers on the top front of the Ion

Max source housing, adjust the side-to-side probe position (see Figure 3).

• Using the A, B, C, and D markers on the probe as a guide, adjust the probe depth.

Figure 3. Top view of the Ion Max source housing with a HESI-II probe

Note The position of the probe port on the Ion Max-S API source housing is not adjustable.

CAUTION Avoid burns. When the system is in operation, the surface of the source

housing can be hot enough to burn your skin. You can touch the micrometer and the

knurled nut while the system is in operation, but avoid touching other parts of the source

housing.

A

S

CAUTION - HOT SURFACE

11

+

0

10

Micrometer

set to 1.75

Side-to-side

position set

to 0

Side-to-side

Knurled nut

Front-to-back

1 Ion Max and Ion Max-S API Source Housings

Removing the API Source Housing

6Ion Max and Ion Max-S API Source Hardware Manual Thermo Scientific

Removing the API Source Housing

You must remove the API source housing to access the APCI corona needle or the ion source

interface.

YTo remove the API source housing

1. Place the mass spectrometer in Standby mode.

2. If the mass spectrometer was recently in operation, allow time for the ion source housing to

cool to room temperature before you touch its external metal surface.

3. If a probe is connected to the source housing, disconnect the external liquid lines before

removing the source housing from the mass spectrometer.

4. Remove the drain tube from the ion source housing drain (see Figure 4).

Figure 4. View of the ion source housing locking levers and drain

5. Rotate the ion source housing locking levers 90 degrees to release the ion source housing from

the ion source mount assembly (see Figure 4).

6. Remove the ion source housing by pulling it straight off of the ion source mount assembly.

Place the housing in a safe location for temporary storage.

Note When the mass spectrometer is in Standby, the high voltage and nitrogen gas are off.

CAUTION Avoid burns. During operation, the external surface of the ion source housing

can become hot. Allow the ion source housing to cool to room temperature before

touching it from the mass spectrometer.

Ion source housing

locking levers

Ion source

housing drain

1 Ion Max and Ion Max-S API Source Housings

Installing the API Source Housing

Thermo Scientific Ion Max and Ion Max-S API Source Hardware Manual 7

Installing the API Source Housing

YTo install the API source housing

1. Carefully align the two guide pin holes on the rear of the ion source housing (see Figure 5)

with the ion source housing guide pins (see Figure 6) on the mass spectrometer. Carefully press

the housing onto the ion source mount.

Figure 5. Rear view of the Ion Max-S ion source housing

Figure 6. Ion source mount assembly with a view of the guide pins

2. Rotate the ion source housing locking levers 90 degrees to lock the ion source housing onto

the ion source mount assembly.

Source housing

locking levers

Guide pin holes

Ion source housing

guide pins

1 Ion Max and Ion Max-S API Source Housings

Installing the API Source Housing

8Ion Max and Ion Max-S API Source Hardware Manual Thermo Scientific

Figure 6 shows the ion sweep cone that has an offset orifice. The ion sweep cone on your ion

source mount assembly might look different from the one shown in Figure 6. Figure 7 shows

the two types of ion sweep cones available on Thermo Scientific mass spectrometers.

Figure 7. Ion sweep cones

3. Reinstall the source drain assembly as follows:

a. Connect the source drain assembly to the ion source housing drain fitting (see “API Source

Housing Drain” on page 9).

b. Attach the free end of the hose to a waste container. Ideally, vent the waste container to a

fume exhaust system.

CAUTION Prevent solvent waste from backing up into the ion source and mass

spectrometer. Always ensure that liquid in the drain tube is able to drain to a waste

container and that the outlet of the drain tube is above the level of liquid in the waste

container.

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Thermo Fisher Scientific Ion Max and Ion Max-S API Source Manuale del proprietario

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