Thermo Fisher Scientific Surveyor UV/Vis Plus Manuale del proprietario

Surveyor UV/Vis Plus

Detector

Hardware Manual

60053-97108 Revision E March 2011

Surveyor, ChromQuest, and Xcalibur are registered trademarks and LightPipe is a trademark of Thermo Fisher

Scientific Inc. in the United States.

The following are registered trademarks in the United States and other countries: Windows is a registered

trademark of Microsoft Corporation. Kel-F is a registered trademark of 3M Company.

PEEK is a trademark of Victrex PLC in the United States and possibly other countries.

All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-

free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might

result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Release history: Revision A, October 2005; Revision B, April 2006; Revision C, March 2008; Revision D,

January 2009; Revision E, March 2011

Software versions: The current converter board firmware for the Surveyor UV/Vis Plus Detector is compatible

with these data systems: ChromQuest versions 4.0 SP1, 4.1 SP2, 4.2, and 5.0 and Xcalibur versions 2.0.5,

2.07, and 2.1.x with Thermo Foundation 1.0.x. You can upload the current firmware with the firmware

upgrade utility.

For Research Use Only. Not for use in diagnostic procedures.

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with

applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent

electromagnetic compatibility (EMC) and safety standards as described in the next section by product name.

Changes that you make to your system might void compliance with one or more of these EMC and safety standards.

Changes to your system include replacing a part or adding components, options, or peripherals not specifically

authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,

replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific

or one of its authorized representatives.

Surveyor UV/Vis Plus Detector

EMC Directive 89/336/EEC amended by 92/31/EEC and 93/68/EEC

EMC compliance has been evaluated by Underwriters Laboratories Inc.

Low Voltage Safety Compliance

This device complies with Low Voltage Directive EN 61010-1:2001.

FCC Compliance Statement

EN 55011 1998 EN 61000-4-3 2002

EN 61000-3-2 1995, A1; 1998, A2; 1998, A14; 2000 EN 61000-4-4 1995, A1; 2001, A2; 2001

IEC 61000-3-2 2000 EN 61000-4-5 1995, A1; 2001

EN 61000-3-3 1995 EN 61000-4-6 1996, A1; 2001

IEC 61000-3-3 1994 EN 61000-4-11 1994, A1; 2001

EN 61326-1 1997 ENV 50204 1995

EN 61000-4-2 1995 A1; 1998 A2; 2001 CISPR 11 1999, A1; 1999, A2; 2002

FCC Class A, CFR 47 Part 15 Subpart B: 2003

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO

THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL

INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,

INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

Notice on Lifting and Handling of

Thermo Scientific Instruments

For your safety, use a team effort to lift or move Thermo Fisher Scientific LC instruments that are too heavy or bulky

for one person to lift or move. In compliance with international regulations, Thermo Fisher Scientific is notifying you

that the Surveyor UV/Vis Detector weighs 19.5 kg (43 lbs).

Notice on the Proper Use of

Thermo Scientific Instruments

In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher

Scientific could impair any protection provided by the instrument.

Notice on the Susceptibility

to Electromagnetic Transmissions

Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency

transmitters, such as mobile phones, in close proximity to the instrument.

For manufacturing location, see the label on the instrument.

CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside

this manual pertaining to the safe use and operation of this product before using the device.

WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE)

Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union

(EU) Member State, and these companies should dispose of or recycle this product. See www.thermo.com/

WEEERoHS for further information on Thermo Fisher Scientific’s compliance with these Directives and the

recyclers in your country.

WEEE Konformität

Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC erfüllen.

Das Produkt ist durch folgendes Symbol gekennzeichnet:

Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten

getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information

über die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise,

die nützlich sind, um die Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter

www.thermo.com/WEEERoHS.

Conformité DEEE

Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et

Electroniques (DEEE). Il est marqué par le symbole suivant:

Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de

l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la

conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les

produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont

disponibles sur www.thermo.com/WEEERoHS.

CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Elektroschock: In diesem Gerät werden

Hochspannungen verwendet, die

Verletzungen verursachen können. Vor

Wartungsarbeiten muß das Gerät

abgeschaltet und vom Netz getrennt

werden. Betreiben Sie Wartungsarbeiten

nicht mit abgenommenem Deckel. Nehmen

Sie die Schutzabdeckung von Leiterplatten

nicht ab.

Choc électrique: L’instrument utilise des

tensions capables d’infliger des blessures

corporelles. L’instrument doit être arrêté et

débranché de la source de courant avant

tout intervention. Ne pas utiliser

l’instrument sans son couvercle. Ne pas

enlever les étuis protecteurs des cartes de

circuits imprimés.

Descarga eléctrica: Este instrumento

utiliza altas tensiones, capaces de

producir lesiones personales. Antes de

dar servicio de mantenimiento al

instrumento, éste debera apagarse y

desconectarse de la línea de alimentacion

eléctrica. No opere el instrumento sin sus

cubiertas exteriores quitadas. No remueva

las cubiertas protectoras de las tarjetas

de circuito impreso.

Shock da folgorazione. L’apparecchio è

alimentato da corrente ad alta tensione

che puo provocare lesioni fisiche. Prima di

effettuare qualsiasi intervento di

manutenzione occorre spegnere ed isolare

l’apparecchio dalla linea elettrica. Non

attivare lo strumento senza lo schermo

superiore. Non togliere i coperchi a

protezione dalle schede di circuito

stampato (PCB).

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Chemikalien: Dieses Gerät kann

gefährliche Chemikalien enthalten. Tragen

Sie Schutzhandschuhe beim Umgang mit

toxischen, karzinogenen, mutagenen oder

ätzenden/reizenden Chemikalien.

Entsorgen Sie verbrauchtes Öl

entsprechend den Vorschriften in den

vorgeschriebenen Behältern.

Chimique: Des produits chimiques

dangereux peuvent se trouver dans

l’instrument. Portez des gants pour

manipuler tous produits chimiques

toxiques, cancérigènes, mutagènes, ou

corrosifs/irritants. Utiliser des récipients

et des procédures homologuées pour se

débarrasser des déchets d’huile.

Química: El instrumento puede contener

productos quimicos peligrosos. Utilice

guantes al manejar productos quimicos

tóxicos, carcinogenos, mutagenos o

corrosivos/irritantes. Utilice recipientes y

procedimientos aprobados para

deshacerse del aceite usado.

Prodotti chimici. Possibile presenza di

sostanze chimiche pericolose

nell’apparecchio. Indossare dei guanti per

maneggiare prodotti chimici tossici,

cancerogeni, mutageni, o

corrosivi/irritanti. Utilizzare contenitori

aprovo e seguire la procedura indicata per

lo smaltimento dei residui di olio.

Heat: Before servicing the instrument,

allow any heated components to cool.

Hitze: Warten Sie erhitzte Komponenten

erst nachdem diese sich abgekühlt haben.

Haute Temperature: Permettre aux

composants chauffés de refroidir avant

tout intervention.

Altas temperaturas: Permita que lop

componentes se enfríen, ante de efectuar

servicio de mantenimiento.

Calore. Attendere che i componenti

riscaldati si raffreddino prima di

effetturare l’intervento di manutenzione.

Fire: Use care when operating the system

in the presence of flammable gases.

Feuer: Beachten Sie die einschlägigen

VorsichtsmaBnahmen, wenn Sie das

System in Gegenwart von entzündbaren

Gasen betreiben.

Incendie: Agir avec précaution lors de

l’utilisation du système en présence de

gaz inflammables.

Fuego: Tenga cuidado al operar el

sistema en presencia de gases

inflamables.

Incendio. Adottare le dovute precauzioni

quando si usa il sistema in presenza di gas

infiammabili.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

Verletzungsgefahr der Augen:

Verspritzte Chemikalien oder kleine

Partikel können Augenverletzungen

verursachen. Tragen Sie beim Umgang mit

Chemikalien oder bei der Wartung des

Gerätes eine Schutzbrille.

Danger pour les yeux: Des projections

chimiques, liquides, ou solides peuvent

être dangereuses pour les yeux. Porter des

lunettes de protection lors de toute

manipulation de produit chimique ou pour

toute intervention sur l’instrument.

Peligro par los ojos: Las salicaduras de

productos químicos o particulas que

salten bruscamente pueden causar

lesiones en los ojos. Utilice anteojos

protectores al mnipular productos

químicos o al darle servicio de

mantenimiento al instrumento.

Pericolo per la vista. Gli schizzi di

prodotti chimici o delle particelle presenti

nell’aria potrebbero causare danni alla

vista. Indossare occhiali protettivi quando

si maneggiano prodotti chimici o si

effettuano interventi di manutenzione

sull’apparecchio.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

Allgemeine Gefahr: Es besteht eine

weitere Gefahr, die nicht in den

vorstehenden Kategorien beschrieben ist.

Dieses Symbol wird im Handbuch

auBerdem dazu verwendet, um den

Benutzer auf Anweisungen hinzuweisen.

Danger général: Indique la présence

d’un risque n’appartenant pas aux

catégories citées plus haut. Ce symbole

figure également sur l’instrument pour

renvoyer l’utilisateur aux instructions du

présent manuel.

Peligro general: Significa que existe un

peligro no incluido en las categorias

anteriores. Este simbolo también se utiliza

en el instrumento par referir al usuario a

las instrucciones contenidas en este

manual.

Pericolo generico. Pericolo non

compreso tra le precedenti categorie.

Questo simbolo è utilizzato inoltre

sull’apparecchio per segnalare all’utente

di consultare le istruzioni descritte nel

presente manuale.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Wenn Sie sich über die Sicherheit eines

Verfahrens im unklaren sind, setzen Sie

sich, bevor Sie fortfahren, mit Ihrer

lokalen technischen

Unterstützungsorganisation für Thermo

Fisher Scientific San Jose Produkte in

Verbindung.

Si la sûreté d’une procédure est

incertaine, avant de continuer, contacter

le plus proche Service Clientèle pour les

produits de Thermo Fisher Scientific San

Jose.

Cuando la certidumbre acerca de un

procedimiento sea dudosa, antes de

proseguir, pongase en contacto con la

Oficina de Asistencia Tecnica local para

los productos de Thermo Fisher Scientific

San Jose.

Quando e in dubbio la misura di sicurezza

per una procedura, prima di continuare, si

prega di mettersi in contatto con il

Servizio di Assistenza Tecnica locale per i

prodotti di Thermo Fisher Scientific San

Jose.

CAUTION Symbol CAUTION

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Heat: Before servicing the instrument,

allow any heated components to cool.

Fire: Use care when operating the system

in the presence of flammable gases.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual ix

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

LightPipe Flow Cell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Front and Back Panels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Installation Checklist. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Unpacking and Inspecting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Placement and Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Installation Tools. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Checking the Power Setting and Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Making the Back Panel Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Connecting the UV/Vis Detector to the Data System Computer . . . . . . . . . 14

Setting the Unit ID . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Making the Contact Closure Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Connecting the Analog Outputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Setting the Analog Output Voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Making Remote Communications Connections . . . . . . . . . . . . . . . . . . . . . . 21

Installing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Powering On the Detector for the First Time . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Chapter 3 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

Recommended Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Maintenance Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Cleaning Detector External Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Contents

xSurveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

LightPipe Flow Cell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Removing the LightPipe Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Storing the LightPipe Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Installing the LightPipe Mounting Assembly. . . . . . . . . . . . . . . . . . . . . . . . . 30

Installing the LightPipe Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Standard Analytical Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Removing the Standard Analytical Flow Cell Assembly . . . . . . . . . . . . . . . . . 33

Installing the Standard Analytical Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . 35

Cleaning the Flow Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Cleaning the Flow Cell with Organic Solvents. . . . . . . . . . . . . . . . . . . . . . . . 36

Cleaning the Flow Cell with Nitric Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Lamp Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Checking the Deuterium Lamp Usage Hours and Replacing the Lamp. . . . . 38

Replacing the Tungsten Lamp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Chapter 4 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

Detector-Related Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Chapter 5 Accessories and Replaceable Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Optional Flow Cells for the UV/Vis Detector . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Appendix A Surveyor Firmware Upgrade Utility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual xi

P

Preface

This manual describes how to set up and maintain the Surveyor™ UV/Vis Plus Detector.

Related Documentation

In addition to the instrument control Help provided with your data system, Thermo Fisher

Scientific provides these documents as PDF files for the Surveyor UV/Vis Plus Detector:

• Surveyor Plus Preinstallation Requirements Guide

• Surveyor Plus Getting Connected Guide

• Surveyor UV/Vis Plus Detector Hardware Manual

• A Surveyor Plus user guide for each data system

Preface

xii Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and

other special notices appear in boxes.

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION

notice is accompanied by an appropriate CAUTION symbol.

CAUTION Alerts you to the presence of hot surfaces and to the potential injury that could

occur were you to come in contact with a specific instrument area.

CAUTION Alerts you to the presence of an eye hazard and the requirement for wearing

protective safety glasses.

CAUTION Alerts you to the presence of a UV light hazard.

UV

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal

performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Preface

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual xiii

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need.

To contact Technical Support

Find software updates and utilities to download at mssupport.thermo.com.

To contact Customer Service for ordering information

To get local contact information for sales or service

Go to www.thermoscientific.com/wps/portal/ts/contactus.

To copy manuals from the Internet

Go to mssupport.thermo.com, agree to the Terms and Conditions, and then click

Customer Manuals in the left margin of the window.

To suggest changes to documentation or to Help

• Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62.

• Send an e-mail message to the Technical Publications Editor at

techpubs-lcms@thermofisher.com.

Phone 800-532-4752

Fax 561-688-8736

E-mail us.techsupport.analyze@thermofisher.com

Knowledge base www.thermokb.com

Phone 800-532-4752

Fax 561-688-8731

E-mail us.customer-support.analyze@thermofisher.com

Web site www.thermo.com/ms

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual 1

1

Introduction

The Surveyor UV/Vis Plus Detector (see Figure 1) is a member of the Surveyor Plus™ family

of LC instruments.

This chapter provides an introduction to the Surveyor UV/Vis Plus Detector.

Figure 1. Surveyor UV/Vis Plus Detector

Contents

•Functional Description

•LightPipe Flow Cell

•Lamps

•Front and Back Panels

•Status LEDs

•Specifications

1 Introduction

Functional Description

2Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Functional Description

The Surveyor UV/Vis Plus Detector is a full-featured, time-programmable,

variable-wavelength UV/Vis (ultraviolet/visible) absorbance detector. It operates in either the

single or dual wavelength mode in the UV or visible ranges.

The UV/Vis detector is a benchtop unit for inclusion into the Surveyor Plus LC System. You

control the detector remotely over an Ethernet communications link from a Thermo

Scientific data system computer.

The UV/Vis detector consists of a dual light source, an optical bench (see Figure 2), dual

photodiode detectors, and several printed circuit boards (PCBs).

Figure 2. Surveyor UV/Vis Plus Detector optical bench

To provide a complete spectrum of ultraviolet (UV) and visible light, the detector uses a

deuterium lamp (D2) for the UV range (190 to 365 nm) and a tungsten lamp (W) for the

visible range (366 to 800 nm). The lamp cover has a safety interlock that reduces the

possibility of human exposure to harmful UV light. When you remove the cover, the lamps

automatically turn off. A computer-controlled shutter selects the deuterium lamp for sample

analyses in the UV range and the tungsten lamp for sample analyses in the visible range. The

light from the selected lamp goes to a holographic grating that disperses the light into a

spectrum. The grating turns to select the programmed wavelength. For precise wavelength

control, a stepper motor controls the alignment of the grating. A system of baffles that reduces

stray light in the optical bench increases the absorbance linearity.

Tungsten

lamp

Deuterium

lamp

Sample

photodiode

Reference

photodiode

Lens

Flow cell

Beamsplitter

Shutter

Grating

1 Introduction

LightPipe Flow Cell

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual 3

From the grating, the light travels to a fiber optic beamsplitter, where a portion of the light is

redirected to a reference photodiode. The signal from the reference photodiode compensates

for changes in lamp intensity and helps maintain consistent performance as the lamp

performance varies. From the beamsplitter, most of the light passes through a lens and then on

through the flow cell and onto the sample photodiode. The analog PC board converts the

intensity counts from the photons falling on the sample photodiode into data that the data

system can process.

Light-emitting diodes (LEDs) on the front of the module provide information about power,

communications, run, and lamp status. See “Status LEDs” on page 7.

You can order the UV/Vis detector with one of these flow cell options:

• Standard analytical flow cell with a 10 mm path length

• LightPipe™ flow cell with a 50 mm path length

You can order other flow cells as options for the detector. See Table 5.

LightPipe Flow Cell

The internal bore of the LightPipe flow cell is 50 mm long and has a volume of 10 μL. The

5 cm optical path length produces a signal five times that of a conventional 1 cm flow cell,

while the low internal volume of 10 μL minimizes band broadening. A special low refractive

index coating of the internal bore ensures a high optical throughput and minimizes

short-term noise.

Mobile phase enters the LightPipe flow cell through a port in the bottom of the flow cell and

exits through a port in the top of the flow cell. See Figure 3. Directing the flow from the

bottom to the top of the flow cell reduces the development of air bubbles.

Figure 3. 50 mm LightPipe flow cell schematic

50 mm flowpath

Inlet port

Alignment slot (top) Outlet port

1 Introduction

LightPipe Flow Cell

4Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Sensitive optical fibers are exposed at both ends of the LightPipe flow cell (see Figure 4).

Thermo Fisher Scientific ships the LightPipe flow cell with end caps (see Figure 5) to protect

these optical fibers. Do not remove the protective end caps until you install the LightPipe flow

cell. If you remove the LightPipe flow cell from the detector, replace the protective end caps

for storage.

Figure 4. End of LightPipe flow cell, showing the sensitive optical fiber

Figure 5. LightPipe flow cell with protective end caps

CAUTION Avoid touching the ends of the LightPipe flow cell. Touching the ends of the

flow cell can damage the exposed optical fibers. If you must grasp the ends of the

LightPipe flow cell, wear clean, talc-free gloves.

Note Thermo Fisher Scientific ships two versions of the LightPipe flow cell. Figure 4

shows one version and Figure 21 and Figure 22 on page 29 show the other version.

Optical fiber

Tubing port

Protective end caps for storage

1 Introduction

Lamps

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual 5

Lamps

The UV/Vis detector has two lamps. The tungsten lamp emits light in the visible region, and

the deuterium lamp emits light in the ultraviolet region. With use, the deuterium lamp emits

less and less light before it fails to ignite (Figure 6). In contrast, the light output from the

tungsten lamp remains relatively constant until the lamp fails.

Figure 6. Deuterium lamp intensity versus lamp usage hours

The deuterium lamp typically requires a warm-up time of 20 to 30 minutes. However, for

applications that demand great sensitivity, allow a warm-up period of 1.5 hours.

The tungsten lamp typically requires only 15 minutes of warm-up time.

The deuterium lamp has a lifetime of approximately 2000 hours and the tungsten lamp has a

lifetime of approximately 2500 hours. Lamp lifetime varies depending on the application.

IMPORTANT The intensity of the deuterium lamp falls off very slightly over a period of

time after it is turned on. Before collecting data in the spectral range of the deuterium

lamp, wait at least 1.5 hours for the lamp to stabilize after a cold start.

Note Turning on the instrument automatically ignites the lamps. If the lamps have been

turned off, you must load a method and wait for the lamps to warm up before you

proceed with a run.

+

0 500 1000 1500 2000

0

20

40

60

80

100

120

Life (Hours)

Relative Intensity

1 Introduction

Front and Back Panels

6Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Front and Back Panels

Figure 7 and Figure 8 show the front and back panels of the UV/Vis detector.

Figure 7. Front panel

Figure 8. Back panel

Power Comm Run Lamps

UV-VIS Plus Detector

S

URVEYOR

Power switch

LED panel

Surveyor UV/VIS Plus Detector

SRVYR-UV5P 5,608,517

50 mm LightPipe/ 3.12

61010 - 1

Model:

Serial No:

Part No:

Options/Firmware

This product is manufactured under, and covered by,

one or more of the following U.S. patents:

355 River Oaks Parkway

San Jose, CA 95134 U.S.A.

This device complies with FCC Rules, Part 15.

Operation is subject to the following two conditions:

(1) This device may not cause harmful interference,

and (2) This device must accept any interference

received, including interference that may cause

undesired operation.

DISCONNECT INPUT

POWER BEFORE

OPENING COVER.

REFER ALL SERVICE

TO QUALIFIED

PERSONNEL ONLY.

CAUTION

T2.5A T1.25A 225VA

100V 115V 230V 50/60Hz

Line

Fuse

ENETUNIT ID

GND B

CHB - 1V

CHB - 100mV

CHB - 10mV

GND A

CHA - 1V

CHA - 100mV

CHA - 10mV

EVENT

GND

ZERO

READY

RUN

NC

+5V @ 150mA

GND

9 10 11 12 13 14 15 16

1 2 3 4 5 6 7 8

RS232

1

2

3

4

5

6

7

8

9

0

1

2

3

4

5

6

7

8

9

0

Analog signal

8-pin connector

Interconnect cable

8-pin connector

Ethernet port

Fuse holderPower

receptacle

Unit ID

rotary switches

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