Thermo Fisher Scientific Surveyor RI Plus Manuale del proprietario

Surveyor RI Plus Detector

Hardware Manual

60253-97000 Revision C January 2009

ChromQuest and Surveyor are trademarks of Thermo Fisher Scientific Inc. in the United States.

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The contents of this document are subject to change without notice. All technical information in this

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Release history: Revision A released November 2006, Revision B released March 2008, Revision C released

January 2009. Revision C contains typographical corrections.

Data system: ChromQuest 4.2 or higher, ChromQuest 5.0 or higher for ChromQuest SI (single instrument)

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug

Administration or other competent authorities.

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with

applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent

electromagnetic compatibility (EMC) and safety standards as described in the next section.

Changes that you make to your system might void compliance with one or more of these EMC and safety standards.

Changes to your system include replacing a part or adding components, options, or peripherals not specifically

authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,

replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific

or one of its authorized representatives.

Surveyor RI Plus Detector

EMC Directive 89/336/EEC, 92/31/EEC, 93/68/EEC

EMC compliance has been evaluated by TUV Rheinland of North America, Inc.

Low Voltage Safety Compliance

Low voltage safety compliance has been evaluated by TUV Rheinland of North America, Inc.

This device complies with Low Voltage Directive 73/23/EEC and harmonized standard EN 61010-1:2001,

IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.

EN55011 1998; A1, 1999; A2, 2002 EN 61000-4-3 2002

CISPR11 1998 EN 61000-4-4 1995, A1; 2001, A2; 2001

EN 61326-1 1997; A1, 1998; A2, 2001; A3, 2003 EN 61000-4-5 2001

EN 61000-3-2 2000 EN 61000-4-6 2003

EN 61000-3-3 1995; A1, 2001 EN 61000-4-11 2001

EN 61000-4-2 2001

FCC Class A, CFR 47 Part 15 Subpart B: 2005

FCC Compliance Statement

Notice on Lifting and Handling of

Thermo Scientific Instruments

For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific

instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one

person alone to handle safely.

Notice on the Proper Use of

Thermo Scientific Instruments

In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher

Scientific could impair any protection provided by the instrument.

Notice on the Susceptibility

to Electromagnetic Transmissions

Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency

transmitters, such as mobile phones, in close proximity to the instrument.

For manufacturing location, see the label on the instrument.

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO

THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL

INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,

INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside

this manual pertaining to the safe use and operation of this product before using the device.

WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic

Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each

European Union (EU) Member State, and these companies should dispose of or recycle this product.

See www.thermo.com/WEEERoHS for further information on Thermo Fisher Scientific’s compliance

with these Directives and the recyclers in your country.

WEEE Konformität

Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC

erfüllen. Das Produkt ist durch folgendes Symbol gekennzeichnet:

Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-

Mitgliedsstaaten getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt

werden kann. Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher

Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die Produkte zu

identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter www.thermo.com/

WEEERoHS.

Conformité DEEE

Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements

Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant:

Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état

membre de l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage

d'informations sur la conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre

pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des

substances sujettes à la directive RoHS sont disponibles sur www.thermo.com/WEEERoHS.

CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Elektroschock: In diesem Gerät werden

Hochspannungen verwendet, die

Verletzungen verursachen können. Vor

Wartungsarbeiten muß das Gerät

abgeschaltet und vom Netz getrennt

werden. Betreiben Sie Wartungsarbeiten

nicht mit abgenommenem Deckel. Nehmen

Sie die Schutzabdeckung von Leiterplatten

nicht ab.

Choc électrique: L’instrument utilise des

tensions capables d’infliger des blessures

corprelles. L’instrument doit être arrêté et

débranché de la source de courant avant

tout intervention. Ne pas utiliser

l’instrument sans son couvercle. Ne pas

elensver les étuis protecteurs des cartes de

circuits imprimés.

Descarga eléctrica: Este instrumento

utiliza altas tensiones, capaces de

producir lesiones personales. Antes de

dar servicio de mantenimiento al

instrumento, éste debera apagarse y

desconectarse de la línea de alimentacion

eléctrica. No opere el instrumento sin sus

cubiertas exteriores quitadas. No remueva

las cubiertas protectoras de las tarjetas

de circuito impreso.

Shock da folgorazione. L’apparecchio è

alimentato da corrente ad alta tensione

che puo provocare lesioni fisiche. Prima di

effettuare qualsiasi intervento di

manutenzione occorre spegnere ed isolare

l’apparecchio dalla linea elettrica. Non

attivare lo strumento senza lo schermo

superiore. Non togliere i coperchi a

protezione dalle schede di circuito

stampato (PCB).

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Chemikalien: Dieses Gerät kann

gefährliche Chemikalien enthalten. Tragen

Sie Schutzhandschuhe beim Umgang mit

toxischen, karzinogenen, mutagenen oder

ätzenden/reizenden Chemikalien.

Entsorgen Sie verbrauchtes Öl

entsprechend den Vorschriften in den

vorgeschriebenen Behältern.

Chimique: Des produits chemiques

dangereux peuven se trouver dans

l’instrument. Proted dos gants pour

manipuler tous produits chemiques

toxiques, cancérigènes, mutagènes, ou

corrosifs/irritants. Utiliser des récipients

et des procédures homologuées pour se

débarrasser des déchets d’huile.

Química: El instrumento puede contener

productos quimicos peligrosos. Utilice

guantes al manejar productos quimicos

tóxicos, carcinogenos, mutagenos o

corrosivos/irritantes. Utilice recipientes y

procedimientos aprobados para

deshacerse del aceite usado.

Prodotti chimici. Possibile presenza di

sostanze chimiche pericolose

nell’apparecchio. Indossare dei guanti per

maneggiare prodotti chimici tossici,

cancerogeni, mutageni, o

corrosivi/irritanti. Utilizzare contenitori

aprovo e seguire la procedura indicata per

lo smaltimento dei residui di olio.

Heat: Before servicing the instrument,

allow any heated components to cool.

Hitze: Warten Sie erhitzte Komponenten

erst nachdem diese sich abgekühlt haben.

Haute Temperature: Permettre aux

composants chauffés de refroidir avant

tout intervention.

Altas temperaturas: Permita que lop

componentes se enfríen, ante de efectuar

servicio de mantenimiento.

Calore. Attendere che i componenti

riscaldati si raffreddino prima di

effetturare l’intervento di manutenzione.

Fire: Use care when operating the system

in the presence of flammable gases.

Feuer: Beachten Sie die einschlägigen

VorsichtsmaBnahmen, wenn Sie das

System in Gegenwart von entzündbaren

Gasen betreiben.

Incendie: Agir avec précaution lors de

l’utilisation du système en présence de

gaz inflammables.

Fuego: Tenga cuidado al operar el

sistema en presencia de gases

inflamables.

Incendio. Adottare le dovute precauzioni

quando si usa il sistema in presenza di gas

infiammabili.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

Verletzungsgefahr der Augen:

Verspritzte Chemikalien oder kleine

Partikel können Augenverletzungen

verursachen. Tragen Sie beim Umgang mit

Chemikalien oder bei der Wartung des

Gerätes eine Schutzbrille.

Danger pour les yeux: Dex projections

chimiques, liquides, ou solides peuvent

être dangereuses pour les yeux. Porter des

lunettes de protection lors de toute

manipulationde produit chimique ou pour

toute intervention sur l’instrument.

Peligro par los ojos: Las salicaduras de

productos químicos o particulas que

salten bruscamente pueden causar

lesiones en los ojos. Utilice anteojos

protectores al mnipular productos

químicos o al darle servicio de

mantenimiento al instrumento.

Pericolo per la vista. Gli schizzi di

prodotti chimici o delle particelle presenti

nell’aria potrebbero causare danni alla

vista. Indossare occhiali protettivi quando

si maneggiano prodotti chimici o si

effettuano interventi di manutenzione

sull’apparecchio.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

Allgemeine Gefahr: Es besteht eine

weitere Gefahr, die nicht in den

vorstehenden Kategorien beschrieben ist.

Dieses Symbol wird im Handbuch

auBerdem dazu verwendet, um den

Benutzer auf Anweisungen hinzuweisen.

Danger général: Indique la présence

d;un risque n’appartenant pas aux

catégories citées plus haut. Ce symbole

figure également sur l’instrument pour

renvoyer l’utilisateur aux instructions du

présent manuel.

Peligro general: Significa que existe un

peligro no incluido en las categorias

anteriores. Este simbolo también se utiliza

en el instrumento par referir al usuario a

las instrucciones contenidas en este

manual.

Pericolo generico. Pericolo non

compreso tra le precedenti categorie.

Questo simbolo è utilizzato inoltre

sull’apparecchio per segnalare all’utente

di consultare le istruzioni descritte nel

presente manuale.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Wenn Sie sich über die Sicherheit eines

Verfahrens im unklaren sind, setzen Sie

sich, bevor Sie fortfahren, mit Ihrer

lokalen technischen

Unterstützungsorganisation für Thermo

Fisher Scientific San Jose Produkte in

Verbindung.

Si la sûreté d’un procédure est incertaine,

avant de continuer, contacter le plus

proche Service Clientèle pour les produits

de Thermo Fisher Scientific San Jose.

Cuando la certidumbre acerca de un

procedimiento sea dudosa, antes de

proseguir, pongase en contacto con la

Oficina de Asistencia Tecnica local para

los productos de Thermo Fisher Scientific

San Jose.

Quando e in dubbio la misura di sicurezza

per una procedura, prima di continuare, si

prega di mettersi in contatto con il

Servizio di Assistenza Tecnica locale per i

prodotti di Thermo Fisher Scientific San

Jose.

CAUTION Symbol CAUTION

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Heat: Before servicing the instrument,

allow any heated components to cool.

Fire: Use care when operating the system

in the presence of flammable gases.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Thermo Scientific Surveyor RI Plus Detector Hardware Manual ix

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Back Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

ChromQuest User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Optical System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Flowcell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Installation Checklist. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Unpacking and Inspecting the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Loosening the Transport Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Integrating the Detector into the LC System . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Making the Initial Back Panel Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Connecting the Ethernet Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Connecting the System Interconnect Cable . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Connecting the Inlet and Outlet Tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Installing the Software Driver . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Configuring the RI Detector in ChromQuest . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Powering On the Detector for the First Time . . . . . . . . . . . . . . . . . . . . . . . . . . 23

Chapter 3 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

General Operating Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Preparing for Routine Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Purging the Flowcell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Calibrating the Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Recorder Output Adjustment Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

Integrator Output Adjustment Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Solvent Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Contents

xSurveyor RI Plus Detector Hardware Manual Thermo Scientific

Chapter 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Recommendations for Routine Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Cleaning Detector External Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Flushing the Flowcell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Replacing the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Chapter 5 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

Detector-Related Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Chapter A Accessories and Replaceable Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Thermo Scientific Surveyor RI Plus Detector Hardware Manual xi

P

Preface

This manual describes how to install and use the Surveyor RI Plus Detector. You can find

additional information in the software Help.

Related Documentation

In addition to this guide, Thermo Fisher Scientific provides the following documents for the

Surveyor RI Plus Detector:

•Surveyor Plus Preinstallation Requirements Guide

•Surveyor Plus Getting Started with ChromQuest Guide

•Surveyor Plus Getting Connected Guide

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and

other special notices appear in boxes.

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each

CAUTION notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal

performance of the system.

Note Highlights information of general interest.

Tip Helpful information that can make a task easier.

Preface

xii Surveyor RI Plus Detector Hardware Manual Thermo Scientific

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need.

YTo contact Technical Support

Find software updates and utilities to download at mssupport.thermo.com.

YTo contact Customer Service for ordering information

YTo copy manuals from the Internet

Go to mssupport.thermo.com and click Customer Manuals in the left margin of the

window.

YTo suggest changes to documentation or to Help

• Complete a brief survey about this document by clicking the link below. Thank you

in advance for your help.

• Send an e-mail message to the Technical Publications Editor at

techpubs-lcms@thermofisher.com.

Phone 800-532-4752

Fax 561-688-8736

E-mail us.techsupport.analyze@thermofisher.com

Knowledge base www.thermokb.com

Phone 800-532-4752

Fax 561-688-8731

E-mail us.customer-support.analyze@thermofisher.com

Web site www.thermo.com/ms

Thermo Scientific Surveyor RI Plus Detector Hardware Manual 1

1

Introduction

The Surveyor™ RI Plus Detector (see Figure 1) is a member of the Surveyor Plus family of LC

instruments. This chapter provides an introduction to the Surveyor RI Plus Detector.

Figure 1. Surveyor RI Plus Detector

Contents

•Features

•Front Panel

•Back Panel

•Status LEDs

•ChromQuest User Interface

•Theory of Operation

•Specifications

1 Introduction

Features

2Surveyor RI Plus Detector Hardware Manual Thermo Scientific

Features

The Surveyor RI Plus Detector is a full-featured, low-maintenance, refractive index detector.

The detector is a bench-top unit for inclusion in the Surveyor Plus LC system. You control the

detector through an Ethernet link to the ChromQuest data system computer.

The Surveyor RI Plus Detector has the following features:

• An automatic intensity adjustment that compensates for the deterioration in sensitivity

due to flowcell contamination or reduced light levels

•A low volume (8μL) flowcell, which reduces band broadening

• A solenoid valve that operates at half its rated voltage and a tungsten lamp that operates at

a reduced voltage to maximize component life

• LEDs (light emitting diodes) that keep you informed of power, communications, run,

and temperature status

• A dynamic operating range of 1.00 to 1.75 RIU, which makes the detector suitable for a

variety of applications

• Integrated instrument control from the ChromQuest data system, which provides

automated temperature control, a rise time filter, a purge function, an autozero function,

and built-in calibration procedures

Front Panel

The front panel of the Surveyor RI Plus Detector provides access to the flowcell connections.

Liquid enters the flowcell through the IN port and exits the flowcell through the OUT port.

The accessory kit for the Surveyor RI Plus Detector contains the following tubing

connections for the IN and OUT ports:

• The connection to the IN port consists of two stainless steel high-pressure fittings and

approximately 27 inches (69 cm) of 0.005-in ID ×1/16 in. OD stainless steel tubing.

• The connection for the OUT port consists of a five foot length of 0.060 in. ID ×1/16 in.

OD, Teflon tubing that has a piece of stainless steel tubing with a high-pressure fitting

inserted into one end.

1 Introduction

Back Panel

Thermo Scientific Surveyor RI Plus Detector Hardware Manual 3

Back Panel

The power entry module, the Ethernet port, and the 8-pin interconnect receptacle are located

on the back panel of the RI detector as shown in Figure 2. The back panel also contains

terminals that allow you to connect a chart recorder or an integrator. See Tab le 1 for a

description of the 16 terminals on the back panel of the RI detector.

Figure 2. Back panel of RI detector

Table 1. Terminals on the back panel of the RI detector (Sheet 1 of 2)

Terminal No. Label Description

1GND Ground terminal

2 +5V@150mA

3NC

4RUN

5 READY Signal out Ready

6 ZERO External Signal in (AutoZero)

7GN Ground terminal

8 PURGE External Signal In (purge On)

9 RECORDER + Recorder out

10 RECORDER - Recorder out

ENET

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

PURGE

GND

ZERO

READY

RUN

NC

+5V @ 150mA

GND

NC

CHASSIS GND

INTEGRATOR --

INTEGRATOR +

NC

CHASSIS GND

RECORDER --

RECORDER +

355 River Oaks Parkway

San Jose, CA 95134 U.S.A.

This device complies with Part 15 of the FCC Rules.

Operation is subject to the following two conditions:

1) This device may not cause harmful interference, and

2) This device must accept any interference received,

including interference that may cause

undesired operation.

61010 - 1

CAUTION

Disconnect Input Power Before Opening Cover

Service by Qualified Personnel

220V T1.6AL 250V 100VA

110V T3.15AL 250V 100VA

FUSE

110V/220V 50/60HZ

LINE

Ethernet

port

8-pin interconnect

receptacle

Fuse

holder

Power entry

module

1 Introduction

Status LEDs

4Surveyor RI Plus Detector Hardware Manual Thermo Scientific

Status LEDs

Four status LEDS—labeled POWER, COMM, RUN, and TEMP—are located on the right

door of the detector. See Figure 3. Tab le 2 lists the states of the LEDs.

Figure 3. Status LEDs

11 CHASSIS GND Ground terminal

12 NC

13 INTEGRATOR + Integrator out

14 INTEGRATOR - Integrator out

15 CHASSIS GND

16 NC

Table 1. Terminals on the back panel of the RI detector (Sheet 2 of 2)

Terminal No. Label Description

Table 2. States of the status LEDs

LED Status Meaning

Power Green The detector is on and receiving power

Comm Amber There is no communication with the data system.

Green Communication to the data system is established.

Run Amber The detector is not ready to start a run.

Flashing amber An error occurred during the run.

Green The detector is ready to initiate a run.

Flashing green A run is in progress and the detector is sending data to

the data system computer.

Temp Amber The temperature of the detector is not equilibrated.

Green The detector has reached a stable.

Power Comm Run Temp

1 Introduction

ChromQuest User Interface

Thermo Scientific Surveyor RI Plus Detector Hardware Manual 5

ChromQuest User Interface

You control the Surveyor RI Plus Detector, as well as the other modules of the Surveyor Plus

LC system, from the ChromQuest chromatography data system.

The ChromQuest user interface has two windows: Main Menu and Instrument. You

administrate and configure the instrument in the Main Menu window. You control the

instrument and process data in the Instrument window.

Figure 4 shows the Main Menu window, the first screen that appears when you launch

ChromQuest from the desktop. Right-clicking the icon for an instrument opens a shortcut

menu with access to the Configuration dialog box. Double-clicking the icon for an

instrument opens the online Instrument window, where you set up methods, create

sequences, and acquire and process data.

To create methods, sequence, and advanced reports during data acquisition, open the offline

Instrument window by choosing Open Offline from the Instrument shortcut menu.

Figure 4. Main Menu window, showing the shortcut menu

Note ChromQuest SI does not contain the Main Menu window and does not provide

security features.

Instrument

icon

Instrument

shortcut menu

1 Introduction

Theory of Operation

6Surveyor RI Plus Detector Hardware Manual Thermo Scientific

Theory of Operation

The Surveyor RI Plus Detector consists of a high-quality optical system and a flowcell. This

section contains the following topics:

•Optical System

•Flowcell

Optical System

The optical design of the Surveyor RI Plus Detector is based on Snell’s Law of Refraction,

which uses a prism flowcell. The detector has a flowcell with two compartments that (the

sample and reference) form a pair of liquid prisms.

A beam of light is directed through the flowcell. As the light passes through the reference and

sample compartments of the flowcell, it is deflected. This deflection is proportional to the

difference in the refractive indices of the reference and sample solvents. The detector converts

the deflection detected by a photodiode into an output signal that it sends to the data system.

ChromQuest converts the output signal from the detector and displays the result in the

dimensionless units of RI. Figure 5 contains a representation of this process and Table 3 lists

the components involved in this process.

Figure 5. Deflection type refractive index detector

Table 3. Deflection type refractive index detector

Item No. Description

1Light

2 Sample compartment

3 Reference compartment

4 Light beam at the time of ns > nr

5 Light beam at the time of ns = nr

6Displacement

7Photodiode

nsRefractive index of liquid in sample compartment

nrRefractive index of liquid in reference compartment

1

nr

ns

2

3

4

5

6 7

1 Introduction

Theory of Operation

Thermo Scientific Surveyor RI Plus Detector Hardware Manual 7

Figure 6 shows a schematic of the optical system. The numbered components shown in

Figure 6 are described in Table 4.

Figure 6. Optical system components

The condensing lens (item 2) and slit no.1 (item 3) form an image of the tungsten filament

(item 1). This image passes through the collimator lens (item 4) and slit no.2 (item 5), which

project the image of slit no. 2 through the flowcell (item 6) and onto the photodiode (item 9).

Table 4. Optical system components

Item No. Description

1Tungsten lamp

2 Condensing lens

3Slit No. 1

4 Collimator lens

5Slit No. 2

6Flowcell

7Mirror

8Null glass

9Photodiode

1 2 3

4 5 6 7

9 8

1 Introduction

Theory of Operation

8Surveyor RI Plus Detector Hardware Manual Thermo Scientific

When there is a difference in the refractive indices of the sample and reference solvents in the

flowcell, the image on the photodiode moves horizontally as illustrated in Figure 7.

Figure 7. Movement of the image on the photodiode

The electrical signals emitted respectively from the diodes A and B change in proportion to

the movement of the image. A signal corresponding to the difference in refractive index can be

obtained from the difference between the two signal outputs.

Flowcell

The flowcell consists of a reference compartment and a sample compartment. During normal

operation, the reference compartment is shut off from the solvent path. You can replace the

solvent in the reference compartment by using the purge control feature provided in the

ChromQuest data system.

Figure 8 shows a block diagram of the flowcell:

•When the Purge Control is On, solvent flows through both the sample and the reference

compartments of the flowcell.

•When the Purge Control is Off, the reference compartment of the flowcell is shut off

from the solvent path.

a. No difference in the

refractive index

Diode A Diode B

Image

b. Some difference in the

refractive index

Diode A Diode B

Image

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