Thermo Fisher Scientific Surveyor Autosampler Plus & Surveyor Autosampler Plus Lite Manuale del proprietario

Surveyor Autosampler Plus &

Surveyor Autosampler Plus Lite

Hardware Manual

60053-97106 Revision E January 2009

Surveyor and ChromQuest are trademarks and Xcalibur is a registered trademark of Thermo Fisher Scientific

Inc. in the United States.

PEEK is a trademark of Victrex PLC. VIEWseal and POWERseal are trademarks of Greiner Bio One.

The following are registered trademarks in the United States and other countries: Windows is a registered

trademark of Microsoft Corporation. Teflon is a registered trademark of E. I. du Pont de Nemours and

Company.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the

product operation. This document is copyright protected and any reproduction of the whole or any part of this

document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this

document is for reference purposes only. System configurations and specifications in this document supersede

all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-

free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might

result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This

document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of

Sale shall govern all conflicting information between the two documents.

Software versions: Xcalibur 2.0 or higher data system, ChromQuest 4.2 or higher data system

Revision history: Revision A April 2005, Revision B May 2006, Revision C September 2006, Revision D

March 2008, Revision E January 2009

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug

Administration or other competent authorities.

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with

applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent

electromagnetic compatibility (EMC) and safety standards as described in the next section.

Changes that you make to your system might void compliance with one or more of these EMC and safety standards.

Changes to your system include replacing a part or adding components, options, or peripherals not specifically

authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,

replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific

or one of its authorized representatives.

Surveyor Autosampler Plus

EMC Directive 89/336/EEC amended by 92/31/EEC and 93/68/EEC

EMC compliance has been evaluated by TUV Rheinland of North America, Inc.

Low Voltage Safety Compliance

Low Voltage Safety Compliance has been evaluated by TUV Rheinland of North America, Inc.

This device complies with Low Voltage Directive 73/23/EEC and harmonized standard EN 61010-1:2001,

IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.

EN 55011 1998 EN 61000-4-3 2002

EN 61000-3-2 1995, A1; 1998, A2; 1998, A14; 2000 EN 61000-4-4 1995, A1; 2001, A2; 2001

IEC 61000-3-2 2000 EN 61000-4-5 1995, A1; 2001

EN 61000-3-3 1995 EN 61000-4-6 1996, A1; 2001

IEC 61000-3-3 1994 EN 61000-4-11 1994, A1; 2001

EN 61326-1 1997

EN 61000-4-2 1995 A1; 1998 A2; 2001 CISPR 11 1999, A1; 1999, A2; 2002

FCC Class A, CFR 47 Part 15 Subpart B: 2003

FCC Compliance Statement

Notice on Lifting and Handling of

Thermo Scientific Instruments

For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific

instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one

person alone to handle safely.

Notice on the Proper Use of

Thermo Scientific Instruments

In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher

Scientific could impair any protection provided by the instrument.

Notice on the Susceptibility

to Electromagnetic Transmissions

Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency

transmitters, such as mobile phones, in close proximity to the instrument.

For manufacturing location, see the label on the instrument.

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO

THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL

INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,

INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside

this manual pertaining to the safe use and operation of this product before using the device.

WEEE Compliance

This product is required to comply with the European Union’s Waste Electrical & Electronic

Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each

European Union (EU) Member State, and these companies should dispose of or recycle this product.

See www.thermo.com/WEEERoHS for further information on Thermo Fisher Scientific’s compliance

with these Directives and the recyclers in your country.

WEEE Konformität

Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC

erfüllen. Das Produkt ist durch folgendes Symbol gekennzeichnet:

Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-

Mitgliedsstaaten getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt

werden kann. Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher

Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die Produkte zu

identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter www.thermo.com/

WEEERoHS.

Conformité DEEE

Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements

Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant:

Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état

membre de l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage

d'informations sur la conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre

pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des

substances sujettes à la directive RoHS sont disponibles sur www.thermo.com/WEEERoHS.

CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Elektroschock: In diesem Gerät werden

Hochspannungen verwendet, die

Verletzungen verursachen können. Vor

Wartungsarbeiten muß das Gerät

abgeschaltet und vom Netz getrennt

werden. Betreiben Sie Wartungsarbeiten

nicht mit abgenommenem Deckel. Nehmen

Sie die Schutzabdeckung von Leiterplatten

nicht ab.

Choc électrique: L’instrument utilise des

tensions capables d’infliger des blessures

corprelles. L’instrument doit être arrêté et

débranché de la source de courant avant

tout intervention. Ne pas utiliser

l’instrument sans son couvercle. Ne pas

elensver les étuis protecteurs des cartes de

circuits imprimés.

Descarga eléctrica: Este instrumento

utiliza altas tensiones, capaces de

producir lesiones personales. Antes de

dar servicio de mantenimiento al

instrumento, éste debera apagarse y

desconectarse de la línea de alimentacion

eléctrica. No opere el instrumento sin sus

cubiertas exteriores quitadas. No remueva

las cubiertas protectoras de las tarjetas

de circuito impreso.

Shock da folgorazione. L’apparecchio è

alimentato da corrente ad alta tensione

che puo provocare lesioni fisiche. Prima di

effettuare qualsiasi intervento di

manutenzione occorre spegnere ed isolare

l’apparecchio dalla linea elettrica. Non

attivare lo strumento senza lo schermo

superiore. Non togliere i coperchi a

protezione dalle schede di circuito

stampato (PCB).

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Chemikalien: Dieses Gerät kann

gefährliche Chemikalien enthalten. Tragen

Sie Schutzhandschuhe beim Umgang mit

toxischen, karzinogenen, mutagenen oder

ätzenden/reizenden Chemikalien.

Entsorgen Sie verbrauchtes Öl

entsprechend den Vorschriften in den

vorgeschriebenen Behältern.

Chimique: Des produits chemiques

dangereux peuven se trouver dans

l’instrument. Proted dos gants pour

manipuler tous produits chemiques

toxiques, cancérigènes, mutagènes, ou

corrosifs/irritants. Utiliser des récipients

et des procédures homologuées pour se

débarrasser des déchets d’huile.

Química: El instrumento puede contener

productos quimicos peligrosos. Utilice

guantes al manejar productos quimicos

tóxicos, carcinogenos, mutagenos o

corrosivos/irritantes. Utilice recipientes y

procedimientos aprobados para

deshacerse del aceite usado.

Prodotti chimici. Possibile presenza di

sostanze chimiche pericolose

nell’apparecchio. Indossare dei guanti per

maneggiare prodotti chimici tossici,

cancerogeni, mutageni, o

corrosivi/irritanti. Utilizzare contenitori

aprovo e seguire la procedura indicata per

lo smaltimento dei residui di olio.

Heat: Before servicing the instrument,

allow any heated components to cool.

Hitze: Warten Sie erhitzte Komponenten

erst nachdem diese sich abgekühlt haben.

Haute Temperature: Permettre aux

composants chauffés de refroidir avant

tout intervention.

Altas temperaturas: Permita que lop

componentes se enfríen, ante de efectuar

servicio de mantenimiento.

Calore. Attendere che i componenti

riscaldati si raffreddino prima di

effetturare l’intervento di manutenzione.

Fire: Use care when operating the system

in the presence of flammable gases.

Feuer: Beachten Sie die einschlägigen

VorsichtsmaBnahmen, wenn Sie das

System in Gegenwart von entzündbaren

Gasen betreiben.

Incendie: Agir avec précaution lors de

l’utilisation du système en présence de

gaz inflammables.

Fuego: Tenga cuidado al operar el

sistema en presencia de gases

inflamables.

Incendio. Adottare le dovute precauzioni

quando si usa il sistema in presenza di gas

infiammabili.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

Verletzungsgefahr der Augen:

Verspritzte Chemikalien oder kleine

Partikel können Augenverletzungen

verursachen. Tragen Sie beim Umgang mit

Chemikalien oder bei der Wartung des

Gerätes eine Schutzbrille.

Danger pour les yeux: Dex projections

chimiques, liquides, ou solides peuvent

être dangereuses pour les yeux. Porter des

lunettes de protection lors de toute

manipulationde produit chimique ou pour

toute intervention sur l’instrument.

Peligro par los ojos: Las salicaduras de

productos químicos o particulas que

salten bruscamente pueden causar

lesiones en los ojos. Utilice anteojos

protectores al mnipular productos

químicos o al darle servicio de

mantenimiento al instrumento.

Pericolo per la vista. Gli schizzi di

prodotti chimici o delle particelle presenti

nell’aria potrebbero causare danni alla

vista. Indossare occhiali protettivi quando

si maneggiano prodotti chimici o si

effettuano interventi di manutenzione

sull’apparecchio.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

Allgemeine Gefahr: Es besteht eine

weitere Gefahr, die nicht in den

vorstehenden Kategorien beschrieben ist.

Dieses Symbol wird im Handbuch

auBerdem dazu verwendet, um den

Benutzer auf Anweisungen hinzuweisen.

Danger général: Indique la présence

d;un risque n’appartenant pas aux

catégories citées plus haut. Ce symbole

figure également sur l’instrument pour

renvoyer l’utilisateur aux instructions du

présent manuel.

Peligro general: Significa que existe un

peligro no incluido en las categorias

anteriores. Este simbolo también se utiliza

en el instrumento par referir al usuario a

las instrucciones contenidas en este

manual.

Pericolo generico. Pericolo non

compreso tra le precedenti categorie.

Questo simbolo è utilizzato inoltre

sull’apparecchio per segnalare all’utente

di consultare le istruzioni descritte nel

presente manuale.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Wenn Sie sich über die Sicherheit eines

Verfahrens im unklaren sind, setzen Sie

sich, bevor Sie fortfahren, mit Ihrer

lokalen technischen

Unterstützungsorganisation für Thermo

Fisher Scientific San Jose Produkte in

Verbindung.

Si la sûreté d’un procédure est incertaine,

avant de continuer, contacter le plus

proche Service Clientèle pour les produits

de Thermo Fisher Scientific San Jose.

Cuando la certidumbre acerca de un

procedimiento sea dudosa, antes de

proseguir, pongase en contacto con la

Oficina de Asistencia Tecnica local para

los productos de Thermo Fisher Scientific

San Jose.

Quando e in dubbio la misura di sicurezza

per una procedura, prima di continuare, si

prega di mettersi in contatto con il

Servizio di Assistenza Tecnica locale per i

prodotti di Thermo Fisher Scientific San

Jose.

CAUTION Symbol CAUTION

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Heat: Before servicing the instrument,

allow any heated components to cool.

Fire: Use care when operating the system

in the presence of flammable gases.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Thermo Scientific Surveyor Autosampler Plus Hardware Manual ix

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii

Safety and Special Notices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv

Good Laboratory Practices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv

Contacting Us. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi

Chapter 1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Autosampler Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

The Tray Compartment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2

The Injection System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3

The Column Oven and Tray Compartment Heater/Cooler. . . . . . . . . . . . . .14

The Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

Injection Modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

No Waste Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16

Partial Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Full Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Injection Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

Getting Ready for an Injection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

Drawing Transport Solvent (Partial Loop and No Waste Injections) . . . . . . . 22

Drawing Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

Pushing Transport Solvent (Partial Loop and No Waste Injections) . . . . . . .25

Loading Sample into the Sample Loop. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

Injecting Sample onto Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28

Post-Injection Events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29

Autosampler Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29

Automated Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30

Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31

Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

Software Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34

Installation Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

Unpacking and Inspecting the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . .36

Making Initial Instrument Preparations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36

Contents

xSurveyor Autosampler Plus Hardware Manual Thermo Scientific

Making the Back Panel Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Connecting the System Interconnect Cable. . . . . . . . . . . . . . . . . . . . . . . . . . 38

Connecting the Ethernet Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Checking the Unit ID Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Connecting the Autosampler to Line Power . . . . . . . . . . . . . . . . . . . . . . . . . 40

Mounting the Syringe Drive Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

Connecting the Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Connecting the Wash Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Connecting the Needle Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Connecting the Autosampler to the Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . 46

Bypassing the Column Oven. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Powering On the Autosampler for the First Time . . . . . . . . . . . . . . . . . . . . . . . 49

Calibrating the Surveyor Autosampler Plus . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Changing the Sample Loop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Chapter 3 Sample Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Standard Tray Type. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Installing the Standard Tray Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

Moving the XYZ Arm to the Back of the Tray Compartment . . . . . . . . . . . . 55

Specialized Tray Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Important Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Recommended Vials, Microplates, and Microplate Covers . . . . . . . . . . . . . . . . 59

Chapter 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

Replacing the Rotor Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Disconnecting the Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Disassembling the Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Cleaning the Stator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Installing a New Rotor Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Reconnecting the Solvent Lines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

Replacing the Flush Solvent Inlet Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Replacing the Transfer Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

Lubricating the Lead Screw of the Syringe Drive Assembly . . . . . . . . . . . . . . . . 73

Troubleshooting Blockage in the Injection System . . . . . . . . . . . . . . . . . . . . . . 74

Testing the Needle Tubing for Blockage . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Testing the Needle for Blockage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Testing the Transfer Tube for Blockage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

Cleaning the Injection Valve or the Sample Loop . . . . . . . . . . . . . . . . . . . . . 78

Replacing the Needle Tubing Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

Setting the Needle to the Removal Position. . . . . . . . . . . . . . . . . . . . . . . . . . 80

Removing the Needle Tubing Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Installing the Needle Tubing Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

Setting the XYZ Arm to the Home Position . . . . . . . . . . . . . . . . . . . . . . . . . 85

Replacing the Needle Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Contents

Thermo Scientific Surveyor Autosampler Plus Hardware Manual xi

Changing the Syringe or Replacing the Inner Plunger . . . . . . . . . . . . . . . . . . . . 86

Setting the Syringe to the Removal Position . . . . . . . . . . . . . . . . . . . . . . . . . 87

Removing the Syringe from the Autosampler . . . . . . . . . . . . . . . . . . . . . . . . 87

Replacing the Inner Plunger of the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . 88

Installing the Syringe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

Setting the Syringe to the Home Position . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Cleaning the Needle Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Replacing a Fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

Maintenance Cycles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

Setting the Maintenance Cycles in Xcalibur. . . . . . . . . . . . . . . . . . . . . . . . . . 92

Setting the Maintenance Cycles in ChromQuest . . . . . . . . . . . . . . . . . . . . . . 93

Chapter 5 Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95

Power Up Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

Error Log in Xcalibur . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

Error Log in ChromQuest. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

Autosampler Validation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Chapter 6 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .101

General Chromatography Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . 102

Autosampler-Specific Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

Chromatography Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

Hardware Problems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

Common Operator Errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Insufficient Wash Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Damaged Needle Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Incorrect Syringe Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Inappropriate Injection Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

Incorrect Dead Volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112

Overtightened Transfer Tube or Wash Tube Fittings . . . . . . . . . . . . . . . . . 112

Determining the Transfer Tubing Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

Chapter 7 Consumables and Service Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115

Consumables. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

Service Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

Autosampler Accessory Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118

Appendix A Firmware Updates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119

Installing the Update Files for ChromQuest . . . . . . . . . . . . . . . . . . . . . . . . . . 119

Preparing the Autosampler for a Firmware Download. . . . . . . . . . . . . . . . . . . 120

Downloading Firmware to the Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . 120

Appendix B Quick Reference Guides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123

Using the Xcalibur Direct Control Commands . . . . . . . . . . . . . . . . . . . . . . . . 125

Using the ChromQuest Direct Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . 127

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129

Thermo Scientific Surveyor Autosampler Plus Hardware Manual xiii

P

Preface

This manual describes the features, installation, and maintenance of the Surveyor

Autosampler Plus™. This manual also describes portions of the Xcalibur™ or ChromQuest™

data systems used to perform diagnostics and maintenance procedures for the autosampler.

Related Documentation

In addition to this guide, Thermo Fisher Scientific provides the following documents for the

Surveyor Plus Autosampler:

•Surveyor Plus Preinstallation Requirements Guide

•Surveyor Plus Getting Connected Guide

•Surveyor Plus Getting Started with Xcalibur Guide

• Surveyor Plus Getting Started with ChromQuest Guide

To access these manuals from your data system computer:

• For LC Devices 2.1.0 or 2.2.0, choose Start > All Programs > Xcalibur > Manuals > LC

Devices > Surveyor.

• For LC Devices 2.2.1 or higher, choose Start > All Programs > Thermo Instruments >

Manuals > LC Devices > Surveyor > PDF link.

• For ChromQuest, the manuals are provided on the software CD or the Surveyor Plus

documentation CD.

Preface

xiv Surveyor Autosampler Plus Hardware Manual Thermo Scientific

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and

other special notices appear in boxes.

Safety and special notices include the following:

Good Laboratory Practices

To obtain optimal performance from your LC system and to prevent personal injury or injury

to the environment, do the following:

• Keep good records.

• Read the manufacturers’ Material Safety Data Sheets (MSDSs) for the chemicals being

used in your laboratory.

• Remove particulate matter from your samples before you inject them into the liquid

chromatograph.

• Use HPLC-grade solvents.

• Connect the drainage tubes from the pump, autosampler, and detector to an appropriate

waste receptacle. Dispose of solvents as specified by local regulations.

Keep Good Records

To help identify and isolate problems with either your equipment or your methodology, for

best results, keep good records of all system conditions (for example, % RSDs on retention

times and peak areas, and peak shape and resolution). At a minimum, keep a chromatogram

of a typical sample and standard mixture, well documented with system conditions, for future

reference. Careful comparison of retention times, peak shapes, peak sensitivity, and baseline

noise can provide valuable clues to identifying and solving future problems.

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION

notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal

performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Preface

Thermo Scientific Surveyor Autosampler Plus Hardware Manual xv

Chemical Toxicity

Although the large volume of toxic and flammable solvents used and stored in laboratories can

be quite dangerous, do not ignore the potential hazards posed by your samples. Take special

care to read and follow all precautions that ensure proper ventilation, storage, handling, and

disposal of both solvents and samples. Become familiar with the toxicity data and potential

hazards associated with all chemicals by referring to the manufacturers’ Material Safety Data

Sheets.

Sample Preparation

Always consider the solubility of your sample in the solvent/mobile phase. Sample

precipitation can plug the column, tubing, or flowcell causing flow restriction. This

obstruction can result in irreparable damage to the system. Particulate matter can be avoided

by filtering the samples through 0.45 or 0.2 micron (or less) filters.

Solvent Requirements

Many chemical manufacturers provide a line of high-purity or HPLC-grade reagents that are

free of chemical impurities. Typically, HPLC-grade solvents do not require filtration. You can

significantly prolong the life and effectiveness of the inlet filters, check valves and seals,

injector, and column by routine filtration of other types of solvents through a 0.45 or

0.2 micron (or less) fluorocarbon filter before placing them in the solvent reservoir bottles.

Choose a mobile phase that is compatible with the sample and column you have selected for

your separation. Remember that some solvents are corrosive to stainless steel.

Solvent Disposal

Make sure you have a solvent waste container or other kind of drain system available at or

below the benchtop level. Most solvents have special disposal requirements and should not be

disposed of directly down a drain. Follow all governmental regulations when disposing of any

chemical.

High-Pressure Systems and Leaks

LC systems operate at high pressures. Because liquids are not highly compressible, they do not

store much energy. Accordingly, the high pressures in an LC system pose little immediate

danger. However, correct any leak that occurs as soon as possible. Always wear eye and skin

protection when operating or maintaining an LC system. Always shut down the system and

return it to atmospheric pressure before attempting any maintenance.

Preface

xvi Surveyor Autosampler Plus Hardware Manual Thermo Scientific

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need.

YTo contact Technical Support

Find software updates and utilities to download at mssupport.thermo.com.

YTo contact Customer Service for ordering information

YTo copy manuals from the Internet

Go to mssupport.thermo.com and click Customer Manuals in the left margin of the

window.

YTo suggest changes to documentation or to Help

• Complete a brief survey about this document by clicking the link below. Thank you

in advance for your help.

• Send an e-mail message to the Technical Publications Editor at

techpubs-lcms@thermofisher.com.

Phone 800-532-4752

Fax 561-688-8736

E-mail us.techsupport.analyze@thermofisher.com

Knowledge base www.thermokb.com

Phone 800-532-4752

Fax 561-688-8731

E-mail us.customer-support.analyze@thermofisher.com

Web site www.thermo.com/ms

Thermo Scientific Surveyor Autosampler Plus Hardware Manual 1

1

Introduction

The Surveyor Autosampler Plus is a member of the Surveyor Plus™ family of

high-performance liquid chromatography instruments. The Surveyor Autosampler Plus (see

Figure 1) holds up to 200 standard vials, three 96-well plates, or three 384-well plates;

contains a built-in column oven (5 to 95 °C); provides tray/sample temperature control

(0 to 60 °C); and is capable of performing automated sample preparation routines.

Figure 1. Surveyor Autosampler Plus

Contents

•Autosampler Components

•Injection Modes

•Injection Sequence

•Autosampler Operation

•Automated Sample Preparation

•Specifications

1 Introduction

Autosampler Components

2Surveyor Autosampler Plus Hardware Manual Thermo Scientific

Autosampler Components

The main components of the Surveyor Autosampler Plus are as follows:

•The Tray Compartment

•The Injection System

•The Column Oven and Tray Compartment Heater/Cooler

•The Status LEDs

The Tray Compartment

Two types of sample trays are supplied with the Surveyor Autosampler Plus: a conventional

sample tray and a microwell carrier. The tray compartment can hold up to five conventional

sample trays or one carrier that holds three microwell plates. The tray compartment also holds

up to four 16 mL capacity reservoir vials that can be used to hold solvent, reagent, or diluent.

The reservoir vials are located behind the wash station and are designated RV1, RV2, RV3,

and RV4. Figure 2 shows an empty tray compartment.

Figure 2. Tray compartment

From the left to the right side of the tray compartment, the five conventional sample trays are

designated A, B, C, D, and E. Each conventional sample tray holds up to 40 standard 1.8 mL

vials for a total capacity of 200 samples. Overlays allow the sample trays to accommodate

different vial sizes.

XYZ arm mechanism

Injection port

1 Introduction

Autosampler Components

Thermo Scientific Surveyor Autosampler Plus Hardware Manual 3

The microwell carrier can hold up to three low-density microplates or up to three

high-density microplates. From the front to the back of the tray compartment, the

microplates are designated A, B, and C.

The low-density microplate contains 96 wells in an 8 × 12 configuration, designated A1

through H12, for a total capacity of 288 samples. Each well can hold up to 250 μL of sample.

The high-density microplate contains 384 wells in a 16 × 24 configuration, designated A1

through P24, for a total capacity of 1152 samples. Each well can hold up to 50 μL of sample.

The tray compartment door contains a magnetic switch. The magnet is located in the door

and the switch is attached to the chassis. When you open the door, the switch signals the

autosampler that the door is open. If you select the Verify door is closed check box when you

configure the autosampler, the XYZ arm automatically moves to the back of the tray

compartment when you open the door so that you can remove trays or replace vials.

Opening the tray compartment door while the autosampler is making an injection does not

interrupt the current run. The XYZ arm moves to the back of the compartment after the

injection is complete and the sequence pauses. Closing the autosampler door allows the

sequence to resume.

The Injection System

Figure 3 shows the injection system of the Surveyor Autosampler Plus. The

major components of the injection system are as follows:

•Wash Bottle and Wash Bottle Tube

•XYZ Arm Mechanism

•Needle Tubing Assembly and Needle Assembly

•Syringe Valve

•Syringe

•Wash Station

•Injection Port of the Autosampler and Transfer Tube

•Injection Valve

•Sample Loop

You can order the replaceable components of the injection system by Thermo Fisher Scientific

part number. See Chapter 7, “Consumables and Service Parts.”

CAUTION Because the XYZ arm moves to the sample position, do not place objects taller

than 4.5 cm (1.8 in.) in the tray compartment.

1 Introduction

Autosampler Components

4Surveyor Autosampler Plus Hardware Manual Thermo Scientific

Figure 3. Autosampler injection components

21

6

5

4

3

Standard

1.8 mL vial

Reservoir

vial

Wash

station

Injection port of

autosampler

Dead

volume

Injection valve

To

column

From

pump

Mobile phase

Wash solvent

Sample

Wash

bottle

Concentric

syringe

Syringe

valve

Needle tubing

assembly

Wash

tube

Wash

tube

Transfer tube Inject

position

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