Thermo Fisher Scientific Surveyor Autosampler Plus & Surveyor Autosampler Plus Lite Manuale del proprietario

Tipo
Manuale del proprietario
Surveyor Autosampler Plus &
Surveyor Autosampler Plus Lite
Hardware Manual
60053-97106 Revision E January 2009
© 2009 Thermo Fisher Scientific Inc. All rights reserved.
Surveyor and ChromQuest are trademarks and Xcalibur is a registered trademark of Thermo Fisher Scientific
Inc. in the United States.
PEEK is a trademark of Victrex PLC. VIEWseal and POWERseal are trademarks of Greiner Bio One.
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Company.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Software versions: Xcalibur 2.0 or higher data system, ChromQuest 4.2 or higher data system
Revision history: Revision A April 2005, Revision B May 2006, Revision C September 2006, Revision D
March 2008, Revision E January 2009
For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug
Administration or other competent authorities.
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent
electromagnetic compatibility (EMC) and safety standards as described in the next section.
Changes that you make to your system might void compliance with one or more of these EMC and safety standards.
Changes to your system include replacing a part or adding components, options, or peripherals not specifically
authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,
replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific
or one of its authorized representatives.
Surveyor Autosampler Plus
EMC Directive 89/336/EEC amended by 92/31/EEC and 93/68/EEC
EMC compliance has been evaluated by TUV Rheinland of North America, Inc.
Low Voltage Safety Compliance
Low Voltage Safety Compliance has been evaluated by TUV Rheinland of North America, Inc.
This device complies with Low Voltage Directive 73/23/EEC and harmonized standard EN 61010-1:2001,
IEC 61010-1:2002, UL 61010 A-1:2004, CAN/CSA 22.2 61010-1:2004.
EN 55011 1998 EN 61000-4-3 2002
EN 61000-3-2 1995, A1; 1998, A2; 1998, A14; 2000 EN 61000-4-4 1995, A1; 2001, A2; 2001
IEC 61000-3-2 2000 EN 61000-4-5 1995, A1; 2001
EN 61000-3-3 1995 EN 61000-4-6 1996, A1; 2001
IEC 61000-3-3 1994 EN 61000-4-11 1994, A1; 2001
EN 61326-1 1997
EN 61000-4-2 1995 A1; 1998 A2; 2001 CISPR 11 1999, A1; 1999, A2; 2002
FCC Class A, CFR 47 Part 15 Subpart B: 2003
FCC Compliance Statement
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific
instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one
person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency
transmitters, such as mobile phones, in close proximity to the instrument.
For manufacturing location, see the label on the instrument.
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO
THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside
this manual pertaining to the safe use and operation of this product before using the device.
WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each
European Union (EU) Member State, and these companies should dispose of or recycle this product.
See www.thermo.com/WEEERoHS for further information on Thermo Fisher Scientific’s compliance
with these Directives and the recyclers in your country.
WEEE Konformität
Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2002/96/EC
erfüllen. Das Produkt ist durch folgendes Symbol gekennzeichnet:
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-
Mitgliedsstaaten getroffen, damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt
werden kann. Mehr Information über die Einhaltung dieser Anweisungen durch Thermo Fisher
Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die Produkte zu
identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter www.thermo.com/
WEEERoHS.
Conformité DEEE
Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements
Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état
membre de l’union européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage
d'informations sur la conformité de Thermo Fisher Scientific à ces directives, les recycleurs dans votre
pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des
substances sujettes à la directive RoHS sont disponibles sur www.thermo.com/WEEERoHS.
CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA
Electric Shock: This instrument uses
high voltages that can cause personal
injury. Before servicing, shut down the
instrument and disconnect the instrument
from line power. Keep the top cover on
while operating the instrument. Do not
remove protective covers from PCBs.
Elektroschock: In diesem Gerät werden
Hochspannungen verwendet, die
Verletzungen verursachen können. Vor
Wartungsarbeiten muß das Gerät
abgeschaltet und vom Netz getrennt
werden. Betreiben Sie Wartungsarbeiten
nicht mit abgenommenem Deckel. Nehmen
Sie die Schutzabdeckung von Leiterplatten
nicht ab.
Choc électrique: L’instrument utilise des
tensions capables d’infliger des blessures
corprelles. L’instrument doit être arrêté et
débranché de la source de courant avant
tout intervention. Ne pas utiliser
l’instrument sans son couvercle. Ne pas
elensver les étuis protecteurs des cartes de
circuits imprimés.
Descarga eléctrica: Este instrumento
utiliza altas tensiones, capaces de
producir lesiones personales. Antes de
dar servicio de mantenimiento al
instrumento, éste debera apagarse y
desconectarse de la línea de alimentacion
eléctrica. No opere el instrumento sin sus
cubiertas exteriores quitadas. No remueva
las cubiertas protectoras de las tarjetas
de circuito impreso.
Shock da folgorazione. L’apparecchio è
alimentato da corrente ad alta tensione
che puo provocare lesioni fisiche. Prima di
effettuare qualsiasi intervento di
manutenzione occorre spegnere ed isolare
l’apparecchio dalla linea elettrica. Non
attivare lo strumento senza lo schermo
superiore. Non togliere i coperchi a
protezione dalle schede di circuito
stampato (PCB).
Chemical: This instrument might contain
hazardous chemicals. Wear gloves when
handling toxic, carcinogenic, mutagenic,
or corrosive or irritant chemicals. Use
approved containers and proper
procedures to dispose waste oil.
Chemikalien: Dieses Gerät kann
gefährliche Chemikalien enthalten. Tragen
Sie Schutzhandschuhe beim Umgang mit
toxischen, karzinogenen, mutagenen oder
ätzenden/reizenden Chemikalien.
Entsorgen Sie verbrauchtes Öl
entsprechend den Vorschriften in den
vorgeschriebenen Behältern.
Chimique: Des produits chemiques
dangereux peuven se trouver dans
l’instrument. Proted dos gants pour
manipuler tous produits chemiques
toxiques, cancérigènes, mutagènes, ou
corrosifs/irritants. Utiliser des récipients
et des procédures homologuées pour se
débarrasser des déchets d’huile.
Química: El instrumento puede contener
productos quimicos peligrosos. Utilice
guantes al manejar productos quimicos
tóxicos, carcinogenos, mutagenos o
corrosivos/irritantes. Utilice recipientes y
procedimientos aprobados para
deshacerse del aceite usado.
Prodotti chimici. Possibile presenza di
sostanze chimiche pericolose
nell’apparecchio. Indossare dei guanti per
maneggiare prodotti chimici tossici,
cancerogeni, mutageni, o
corrosivi/irritanti. Utilizzare contenitori
aprovo e seguire la procedura indicata per
lo smaltimento dei residui di olio.
Heat: Before servicing the instrument,
allow any heated components to cool.
Hitze: Warten Sie erhitzte Komponenten
erst nachdem diese sich abgekühlt haben.
Haute Temperature: Permettre aux
composants chauffés de refroidir avant
tout intervention.
Altas temperaturas: Permita que lop
componentes se enfríen, ante de efectuar
servicio de mantenimiento.
Calore. Attendere che i componenti
riscaldati si raffreddino prima di
effetturare l’intervento di manutenzione.
Fire: Use care when operating the system
in the presence of flammable gases.
Feuer: Beachten Sie die einschlägigen
VorsichtsmaBnahmen, wenn Sie das
System in Gegenwart von entzündbaren
Gasen betreiben.
Incendie: Agir avec précaution lors de
l’utilisation du système en présence de
gaz inflammables.
Fuego: Tenga cuidado al operar el
sistema en presencia de gases
inflamables.
Incendio. Adottare le dovute precauzioni
quando si usa il sistema in presenza di gas
infiammabili.
Eye Hazard: Eye damage could occur
from splattered chemicals or flying
particles. Wear safety glasses when
handling chemicals or servicing the
instrument.
Verletzungsgefahr der Augen:
Verspritzte Chemikalien oder kleine
Partikel können Augenverletzungen
verursachen. Tragen Sie beim Umgang mit
Chemikalien oder bei der Wartung des
Gerätes eine Schutzbrille.
Danger pour les yeux: Dex projections
chimiques, liquides, ou solides peuvent
être dangereuses pour les yeux. Porter des
lunettes de protection lors de toute
manipulationde produit chimique ou pour
toute intervention sur l’instrument.
Peligro par los ojos: Las salicaduras de
productos químicos o particulas que
salten bruscamente pueden causar
lesiones en los ojos. Utilice anteojos
protectores al mnipular productos
químicos o al darle servicio de
mantenimiento al instrumento.
Pericolo per la vista. Gli schizzi di
prodotti chimici o delle particelle presenti
nell’aria potrebbero causare danni alla
vista. Indossare occhiali protettivi quando
si maneggiano prodotti chimici o si
effettuano interventi di manutenzione
sull’apparecchio.
General Hazard: A hazard is present that
is not included in the above categories.
Also, this symbol appears on the
instrument to refer the user to instructions
in this manual.
Allgemeine Gefahr: Es besteht eine
weitere Gefahr, die nicht in den
vorstehenden Kategorien beschrieben ist.
Dieses Symbol wird im Handbuch
auBerdem dazu verwendet, um den
Benutzer auf Anweisungen hinzuweisen.
Danger général: Indique la présence
d;un risque n’appartenant pas aux
catégories citées plus haut. Ce symbole
figure également sur l’instrument pour
renvoyer l’utilisateur aux instructions du
présent manuel.
Peligro general: Significa que existe un
peligro no incluido en las categorias
anteriores. Este simbolo también se utiliza
en el instrumento par referir al usuario a
las instrucciones contenidas en este
manual.
Pericolo generico. Pericolo non
compreso tra le precedenti categorie.
Questo simbolo è utilizzato inoltre
sull’apparecchio per segnalare all’utente
di consultare le istruzioni descritte nel
presente manuale.
When the safety of a procedure is
questionable, contact your local Technical
Support organization for Thermo Fisher
Scientific San Jose Products.
Wenn Sie sich über die Sicherheit eines
Verfahrens im unklaren sind, setzen Sie
sich, bevor Sie fortfahren, mit Ihrer
lokalen technischen
Unterstützungsorganisation für Thermo
Fisher Scientific San Jose Produkte in
Verbindung.
Si la sûreté d’un procédure est incertaine,
avant de continuer, contacter le plus
proche Service Clientèle pour les produits
de Thermo Fisher Scientific San Jose.
Cuando la certidumbre acerca de un
procedimiento sea dudosa, antes de
proseguir, pongase en contacto con la
Oficina de Asistencia Tecnica local para
los productos de Thermo Fisher Scientific
San Jose.
Quando e in dubbio la misura di sicurezza
per una procedura, prima di continuare, si
prega di mettersi in contatto con il
Servizio di Assistenza Tecnica locale per i
prodotti di Thermo Fisher Scientific San
Jose.
CAUTION Symbol CAUTION
Electric Shock: This instrument uses
high voltages that can cause personal
injury. Before servicing, shut down the
instrument and disconnect the instrument
from line power. Keep the top cover on
while operating the instrument. Do not
remove protective covers from PCBs.
Chemical: This instrument might contain
hazardous chemicals. Wear gloves when
handling toxic, carcinogenic, mutagenic,
or corrosive or irritant chemicals. Use
approved containers and proper
procedures to dispose waste oil.
Heat: Before servicing the instrument,
allow any heated components to cool.
Fire: Use care when operating the system
in the presence of flammable gases.
Eye Hazard: Eye damage could occur
from splattered chemicals or flying
particles. Wear safety glasses when
handling chemicals or servicing the
instrument.
General Hazard: A hazard is present that
is not included in the above categories.
Also, this symbol appears on the
instrument to refer the user to instructions
in this manual.
When the safety of a procedure is
questionable, contact your local Technical
Support organization for Thermo Fisher
Scientific San Jose Products.
Thermo Scientific Surveyor Autosampler Plus Hardware Manual ix
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
Safety and Special Notices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Good Laboratory Practices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiv
Contacting Us. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Chapter 1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Autosampler Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
The Tray Compartment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
The Injection System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
The Column Oven and Tray Compartment Heater/Cooler. . . . . . . . . . . . . .14
The Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Injection Modes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
No Waste Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Partial Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Full Loop Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Injection Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
Getting Ready for an Injection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Drawing Transport Solvent (Partial Loop and No Waste Injections) . . . . . . . 22
Drawing Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
Pushing Transport Solvent (Partial Loop and No Waste Injections) . . . . . . .25
Loading Sample into the Sample Loop. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Injecting Sample onto Column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Post-Injection Events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Autosampler Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Automated Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Software Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
Installation Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Unpacking and Inspecting the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
Making Initial Instrument Preparations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
Contents
Contents
xSurveyor Autosampler Plus Hardware Manual Thermo Scientific
Making the Back Panel Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Connecting the System Interconnect Cable. . . . . . . . . . . . . . . . . . . . . . . . . . 38
Connecting the Ethernet Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Checking the Unit ID Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Connecting the Autosampler to Line Power . . . . . . . . . . . . . . . . . . . . . . . . . 40
Mounting the Syringe Drive Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Connecting the Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Connecting the Wash Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Connecting the Needle Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Connecting the Autosampler to the Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Bypassing the Column Oven. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Powering On the Autosampler for the First Time . . . . . . . . . . . . . . . . . . . . . . . 49
Calibrating the Surveyor Autosampler Plus . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Changing the Sample Loop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Chapter 3 Sample Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
Standard Tray Type. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Installing the Standard Tray Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Moving the XYZ Arm to the Back of the Tray Compartment . . . . . . . . . . . . 55
Specialized Tray Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Important Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Recommended Vials, Microplates, and Microplate Covers . . . . . . . . . . . . . . . . 59
Chapter 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61
Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Replacing the Rotor Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Disconnecting the Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Disassembling the Injection Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Cleaning the Stator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Installing a New Rotor Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Reconnecting the Solvent Lines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Replacing the Flush Solvent Inlet Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Replacing the Transfer Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Lubricating the Lead Screw of the Syringe Drive Assembly . . . . . . . . . . . . . . . . 73
Troubleshooting Blockage in the Injection System . . . . . . . . . . . . . . . . . . . . . . 74
Testing the Needle Tubing for Blockage . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Testing the Needle for Blockage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Testing the Transfer Tube for Blockage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Cleaning the Injection Valve or the Sample Loop . . . . . . . . . . . . . . . . . . . . . 78
Replacing the Needle Tubing Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
Setting the Needle to the Removal Position. . . . . . . . . . . . . . . . . . . . . . . . . . 80
Removing the Needle Tubing Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Installing the Needle Tubing Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Setting the XYZ Arm to the Home Position . . . . . . . . . . . . . . . . . . . . . . . . . 85
Replacing the Needle Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Contents
Thermo Scientific Surveyor Autosampler Plus Hardware Manual xi
Changing the Syringe or Replacing the Inner Plunger . . . . . . . . . . . . . . . . . . . . 86
Setting the Syringe to the Removal Position . . . . . . . . . . . . . . . . . . . . . . . . . 87
Removing the Syringe from the Autosampler . . . . . . . . . . . . . . . . . . . . . . . . 87
Replacing the Inner Plunger of the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Installing the Syringe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
Setting the Syringe to the Home Position . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Cleaning the Needle Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
Replacing a Fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Maintenance Cycles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Setting the Maintenance Cycles in Xcalibur. . . . . . . . . . . . . . . . . . . . . . . . . . 92
Setting the Maintenance Cycles in ChromQuest . . . . . . . . . . . . . . . . . . . . . . 93
Chapter 5 Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
Power Up Diagnostics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Error Log in Xcalibur . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Error Log in ChromQuest. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Autosampler Validation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
Chapter 6 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .101
General Chromatography Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . 102
Autosampler-Specific Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Chromatography Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Hardware Problems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Common Operator Errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Insufficient Wash Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Damaged Needle Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Incorrect Syringe Type . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Inappropriate Injection Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Incorrect Dead Volume. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Overtightened Transfer Tube or Wash Tube Fittings . . . . . . . . . . . . . . . . . 112
Determining the Transfer Tubing Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Chapter 7 Consumables and Service Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
Consumables. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Service Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
Autosampler Accessory Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Appendix A Firmware Updates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119
Installing the Update Files for ChromQuest . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Preparing the Autosampler for a Firmware Download. . . . . . . . . . . . . . . . . . . 120
Downloading Firmware to the Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . . 120
Appendix B Quick Reference Guides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123
Using the Xcalibur Direct Control Commands . . . . . . . . . . . . . . . . . . . . . . . . 125
Using the ChromQuest Direct Commands . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129
Thermo Scientific Surveyor Autosampler Plus Hardware Manual xiii
P
Preface
This manual describes the features, installation, and maintenance of the Surveyor
Autosampler Plus™. This manual also describes portions of the Xcalibur™ or ChromQuest™
data systems used to perform diagnostics and maintenance procedures for the autosampler.
Related Documentation
In addition to this guide, Thermo Fisher Scientific provides the following documents for the
Surveyor Plus Autosampler:
Surveyor Plus Preinstallation Requirements Guide
Surveyor Plus Getting Connected Guide
Surveyor Plus Getting Started with Xcalibur Guide
Surveyor Plus Getting Started with ChromQuest Guide
To access these manuals from your data system computer:
For LC Devices 2.1.0 or 2.2.0, choose Start > All Programs > Xcalibur > Manuals > LC
Devices > Surveyor.
For LC Devices 2.2.1 or higher, choose Start > All Programs > Thermo Instruments >
Manuals > LC Devices > Surveyor > PDF link.
For ChromQuest, the manuals are provided on the software CD or the Surveyor Plus
documentation CD.
Preface
xiv Surveyor Autosampler Plus Hardware Manual Thermo Scientific
Safety and Special Notices
Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.
Safety and special notices include the following:
Good Laboratory Practices
To obtain optimal performance from your LC system and to prevent personal injury or injury
to the environment, do the following:
Keep good records.
Read the manufacturers’ Material Safety Data Sheets (MSDSs) for the chemicals being
used in your laboratory.
Remove particulate matter from your samples before you inject them into the liquid
chromatograph.
Use HPLC-grade solvents.
Connect the drainage tubes from the pump, autosampler, and detector to an appropriate
waste receptacle. Dispose of solvents as specified by local regulations.
Keep Good Records
To help identify and isolate problems with either your equipment or your methodology, for
best results, keep good records of all system conditions (for example, % RSDs on retention
times and peak areas, and peak shape and resolution). At a minimum, keep a chromatogram
of a typical sample and standard mixture, well documented with system conditions, for future
reference. Careful comparison of retention times, peak shapes, peak sensitivity, and baseline
noise can provide valuable clues to identifying and solving future problems.
CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Highlights information of general interest.
Tip Highlights helpful information that can make a task easier.
Preface
Thermo Scientific Surveyor Autosampler Plus Hardware Manual xv
Chemical Toxicity
Although the large volume of toxic and flammable solvents used and stored in laboratories can
be quite dangerous, do not ignore the potential hazards posed by your samples. Take special
care to read and follow all precautions that ensure proper ventilation, storage, handling, and
disposal of both solvents and samples. Become familiar with the toxicity data and potential
hazards associated with all chemicals by referring to the manufacturers’ Material Safety Data
Sheets.
Sample Preparation
Always consider the solubility of your sample in the solvent/mobile phase. Sample
precipitation can plug the column, tubing, or flowcell causing flow restriction. This
obstruction can result in irreparable damage to the system. Particulate matter can be avoided
by filtering the samples through 0.45 or 0.2 micron (or less) filters.
Solvent Requirements
Many chemical manufacturers provide a line of high-purity or HPLC-grade reagents that are
free of chemical impurities. Typically, HPLC-grade solvents do not require filtration. You can
significantly prolong the life and effectiveness of the inlet filters, check valves and seals,
injector, and column by routine filtration of other types of solvents through a 0.45 or
0.2 micron (or less) fluorocarbon filter before placing them in the solvent reservoir bottles.
Choose a mobile phase that is compatible with the sample and column you have selected for
your separation. Remember that some solvents are corrosive to stainless steel.
Solvent Disposal
Make sure you have a solvent waste container or other kind of drain system available at or
below the benchtop level. Most solvents have special disposal requirements and should not be
disposed of directly down a drain. Follow all governmental regulations when disposing of any
chemical.
High-Pressure Systems and Leaks
LC systems operate at high pressures. Because liquids are not highly compressible, they do not
store much energy. Accordingly, the high pressures in an LC system pose little immediate
danger. However, correct any leak that occurs as soon as possible. Always wear eye and skin
protection when operating or maintaining an LC system. Always shut down the system and
return it to atmospheric pressure before attempting any maintenance.
Preface
xvi Surveyor Autosampler Plus Hardware Manual Thermo Scientific
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
YTo contact Technical Support
Find software updates and utilities to download at mssupport.thermo.com.
YTo contact Customer Service for ordering information
YTo copy manuals from the Internet
Go to mssupport.thermo.com and click Customer Manuals in the left margin of the
window.
YTo suggest changes to documentation or to Help
Complete a brief survey about this document by clicking the link below. Thank you
in advance for your help.
Send an e-mail message to the Technical Publications Editor at
techpubs-lcms@thermofisher.com.
Phone 800-532-4752
Fax 561-688-8736
E-mail us.techsupport.analyze@thermofisher.com
Knowledge base www.thermokb.com
Phone 800-532-4752
Fax 561-688-8731
E-mail us.customer-support.analyze@thermofisher.com
Web site www.thermo.com/ms
Thermo Scientific Surveyor Autosampler Plus Hardware Manual 1
1
Introduction
The Surveyor Autosampler Plus is a member of the Surveyor Plus™ family of
high-performance liquid chromatography instruments. The Surveyor Autosampler Plus (see
Figure 1) holds up to 200 standard vials, three 96-well plates, or three 384-well plates;
contains a built-in column oven (5 to 95 °C); provides tray/sample temperature control
(0 to 60 °C); and is capable of performing automated sample preparation routines.
Figure 1. Surveyor Autosampler Plus
Contents
Autosampler Components
Injection Modes
Injection Sequence
Autosampler Operation
Automated Sample Preparation
Specifications
1 Introduction
Autosampler Components
2Surveyor Autosampler Plus Hardware Manual Thermo Scientific
Autosampler Components
The main components of the Surveyor Autosampler Plus are as follows:
The Tray Compartment
The Injection System
The Column Oven and Tray Compartment Heater/Cooler
The Status LEDs
The Tray Compartment
Two types of sample trays are supplied with the Surveyor Autosampler Plus: a conventional
sample tray and a microwell carrier. The tray compartment can hold up to five conventional
sample trays or one carrier that holds three microwell plates. The tray compartment also holds
up to four 16 mL capacity reservoir vials that can be used to hold solvent, reagent, or diluent.
The reservoir vials are located behind the wash station and are designated RV1, RV2, RV3,
and RV4. Figure 2 shows an empty tray compartment.
Figure 2. Tray compartment
From the left to the right side of the tray compartment, the five conventional sample trays are
designated A, B, C, D, and E. Each conventional sample tray holds up to 40 standard 1.8 mL
vials for a total capacity of 200 samples. Overlays allow the sample trays to accommodate
different vial sizes.
XYZ arm mechanism
Injection port
1 Introduction
Autosampler Components
Thermo Scientific Surveyor Autosampler Plus Hardware Manual 3
The microwell carrier can hold up to three low-density microplates or up to three
high-density microplates. From the front to the back of the tray compartment, the
microplates are designated A, B, and C.
The low-density microplate contains 96 wells in an 8 × 12 configuration, designated A1
through H12, for a total capacity of 288 samples. Each well can hold up to 250 μL of sample.
The high-density microplate contains 384 wells in a 16 × 24 configuration, designated A1
through P24, for a total capacity of 1152 samples. Each well can hold up to 50 μL of sample.
The tray compartment door contains a magnetic switch. The magnet is located in the door
and the switch is attached to the chassis. When you open the door, the switch signals the
autosampler that the door is open. If you select the Verify door is closed check box when you
configure the autosampler, the XYZ arm automatically moves to the back of the tray
compartment when you open the door so that you can remove trays or replace vials.
Opening the tray compartment door while the autosampler is making an injection does not
interrupt the current run. The XYZ arm moves to the back of the compartment after the
injection is complete and the sequence pauses. Closing the autosampler door allows the
sequence to resume.
The Injection System
Figure 3 shows the injection system of the Surveyor Autosampler Plus. The
major components of the injection system are as follows:
Wash Bottle and Wash Bottle Tube
XYZ Arm Mechanism
Needle Tubing Assembly and Needle Assembly
Syringe Valve
Syringe
Wash Station
Injection Port of the Autosampler and Transfer Tube
Injection Valve
Sample Loop
You can order the replaceable components of the injection system by Thermo Fisher Scientific
part number. See Chapter 7, “Consumables and Service Parts.
CAUTION Because the XYZ arm moves to the sample position, do not place objects taller
than 4.5 cm (1.8 in.) in the tray compartment.
1 Introduction
Autosampler Components
4Surveyor Autosampler Plus Hardware Manual Thermo Scientific
Figure 3. Autosampler injection components
21
6
5
4
3
Standard
1.8 mL vial
Reservoir
vial
Wash
station
Injection port of
autosampler
Dead
volume
Injection valve
To
column
From
pump
Mobile phase
Wash solvent
Sample
Wash
bottle
Concentric
syringe
Syringe
valve
Needle tubing
assembly
Wash
tube
Wash
tube
Transfer tube Inject
position
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