Thermo Fisher Scientific Surveyor UV/Vis Plus Manuale del proprietario

Surveyor UV/Vis Plus

Hardware Manual

60053-97108 Revision C March 2008

Surveyor Plus™and ChromQuest™ are trademarks of Thermo Fisher Scientific Inc. in the United States.

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The contents of this document are subject to change without notice. All technical information in this

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all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-

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Release history: Revision A released in October 2005. Revision B released in April 2006. Revision C released in

March 2008.

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug

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This product is required to comply with the European Union’s Waste Electrical & Electronic

Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each

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See www.thermo.com/WEEERoHS for further information on Thermo Fisher Scientific’s compliance

with these Directives and the recyclers in your country.

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substances sujettes à la directive RoHS sont disponibles sur www.thermo.com/WEEERoHS.

Regulatory Compliance

Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with

applicable domestic and international regulations. When the system is delivered to you, it meets all pertinent

electromagnetic compatibility (EMC) and safety standards as described below.

EMC Directive 89/336/EEC amended by 92/31/EEC and 93/68/EEC

EMC compliance has been evaluated by Underwriters Laboratories Inc.

Low Voltage Safety Compliance

This device complies with Low Voltage Directive EN 61010-1:2001.

Changes that you make to your system may void compliance with one or more of these EMC and safety standards.

Changes to your system include replacing a part or adding components, options, or peripherals not specifically

authorized and qualified by Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards,

replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific

or one of its authorized representatives.

FCC Compliance Statement

EN 55011 1998 EN 61000-4-3 2002

EN 61000-3-2 1995, A1; 1998, A2; 1998, A14; 2000 EN 61000-4-4 1995, A1; 2001, A2; 2001

IEC 61000-3-2 2000 EN 61000-4-5 1995, A1; 2001

EN 61000-3-3 1995 EN 61000-4-6 1996, A1; 2001

IEC 61000-3-3 1994 EN 61000-4-11 1994, A1; 2001

EN 61326-1 1997 ENV 50204 1995

EN 61000-4-2 1995 A1; 1998 A2; 2001 CISPR 11 1999, A1; 1999, A2; 2002

FCC Class A, CFR 47 Part 15 Subpart B: 2003

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO

THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL

INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,

INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

Notice on Lifting and Handling of

Thermo Scientific Instruments

For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific

instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/or bulky for one

person alone to handle safely.

Notice on the Proper Use of

Thermo Scientific Instruments

In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher

Scientific could impair any protection provided by the instrument.

Notice on the Susceptibility

to Electromagnetic Transmissions

Your instrument is designed to work in a controlled electromagnetic environment. Do not use radio frequency

transmitters, such as mobile phones, in close proximity to the instrument.

For manufacturing location, see the label on the instrument.

CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside

this manual pertaining to the safe use and operation of this product before using the device.

CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Elektroschock: In diesem Gerät werden

Hochspannungen verwendet, die

Verletzungen verursachen können. Vor

Wartungsarbeiten muß das Gerät

abgeschaltet und vom Netz getrennt

werden. Betreiben Sie Wartungsarbeiten

nicht mit abgenommenem Deckel. Nehmen

Sie die Schutzabdeckung von Leiterplatten

nicht ab.

Choc électrique: L’instrument utilise des

tensions capables d’infliger des blessures

corprelles. L’instrument doit être arrêté et

débranché de la source de courant avant

tout intervention. Ne pas utiliser

l’instrument sans son couvercle. Ne pas

elensver les étuis protecteurs des cartes de

circuits imprimés.

Descarga eléctrica: Este instrumento

utiliza altas tensiones, capaces de

producir lesiones personales. Antes de

dar servicio de mantenimiento al

instrumento, éste debera apagarse y

desconectarse de la línea de alimentacion

eléctrica. No opere el instrumento sin sus

cubiertas exteriores quitadas. No remueva

las cubiertas protectoras de las tarjetas

de circuito impreso.

Shock da folgorazione. L’apparecchio è

alimentato da corrente ad alta tensione

che puo provocare lesioni fisiche. Prima di

effettuare qualsiasi intervento di

manutenzione occorre spegnere ed isolare

l’apparecchio dalla linea elettrica. Non

attivare lo strumento senza lo schermo

superiore. Non togliere i coperchi a

protezione dalle schede di circuito

stampato (PCB).

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Chemikalien: Dieses Gerät kann

gefährliche Chemikalien enthalten. Tragen

Sie Schutzhandschuhe beim Umgang mit

toxischen, karzinogenen, mutagenen oder

ätzenden/reizenden Chemikalien.

Entsorgen Sie verbrauchtes Öl

entsprechend den Vorschriften in den

vorgeschriebenen Behältern.

Chimique: Des produits chemiques

dangereux peuven se trouver dans

l’instrument. Proted dos gants pour

manipuler tous produits chemiques

toxiques, cancérigènes, mutagènes, ou

corrosifs/irritants. Utiliser des récipients

et des procédures homologuées pour se

débarrasser des déchets d’huile.

Química: El instrumento puede contener

productos quimicos peligrosos. Utilice

guantes al manejar productos quimicos

tóxicos, carcinogenos, mutagenos o

corrosivos/irritantes. Utilice recipientes y

procedimientos aprobados para

deshacerse del aceite usado.

Prodotti chimici. Possibile presenza di

sostanze chimiche pericolose

nell’apparecchio. Indossare dei guanti per

maneggiare prodotti chimici tossici,

cancerogeni, mutageni, o

corrosivi/irritanti. Utilizzare contenitori

aprovo e seguire la procedura indicata per

lo smaltimento dei residui di olio.

Heat: Before servicing the instrument,

allow any heated components to cool.

Hitze: Warten Sie erhitzte Komponenten

erst nachdem diese sich abgekühlt haben.

Haute Temperature: Permettre aux

composants chauffés de refroidir avant

tout intervention.

Altas temperaturas: Permita que lop

componentes se enfríen, ante de efectuar

servicio de mantenimiento.

Calore. Attendere che i componenti

riscaldati si raffreddino prima di

effetturare l’intervento di manutenzione.

Fire: Use care when operating the system

in the presence of flammable gases.

Feuer: Beachten Sie die einschlägigen

VorsichtsmaBnahmen, wenn Sie das

System in Gegenwart von entzündbaren

Gasen betreiben.

Incendie: Agir avec précaution lors de

l’utilisation du système en présence de

gaz inflammables.

Fuego: Tenga cuidado al operar el

sistema en presencia de gases

inflamables.

Incendio. Adottare le dovute precauzioni

quando si usa il sistema in presenza di gas

infiammabili.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

Verletzungsgefahr der Augen:

Verspritzte Chemikalien oder kleine

Partikel können Augenverletzungen

verursachen. Tragen Sie beim Umgang mit

Chemikalien oder bei der Wartung des

Gerätes eine Schutzbrille.

Danger pour les yeux: Dex projections

chimiques, liquides, ou solides peuvent

être dangereuses pour les yeux. Porter des

lunettes de protection lors de toute

manipulationde produit chimique ou pour

toute intervention sur l’instrument.

Peligro par los ojos: Las salicaduras de

productos químicos o particulas que

salten bruscamente pueden causar

lesiones en los ojos. Utilice anteojos

protectores al mnipular productos

químicos o al darle servicio de

mantenimiento al instrumento.

Pericolo per la vista. Gli schizzi di

prodotti chimici o delle particelle presenti

nell’aria potrebbero causare danni alla

vista. Indossare occhiali protettivi quando

si maneggiano prodotti chimici o si

effettuano interventi di manutenzione

sull’apparecchio.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

Allgemeine Gefahr: Es besteht eine

weitere Gefahr, die nicht in den

vorstehenden Kategorien beschrieben ist.

Dieses Symbol wird im Handbuch

auBerdem dazu verwendet, um den

Benutzer auf Anweisungen hinzuweisen.

Danger général: Indique la présence

d;un risque n’appartenant pas aux

catégories citées plus haut. Ce symbole

figure également sur l’instrument pour

renvoyer l’utilisateur aux instructions du

présent manuel.

Peligro general: Significa que existe un

peligro no incluido en las categorias

anteriores. Este simbolo también se utiliza

en el instrumento par referir al usuario a

las instrucciones contenidas en este

manual.

Pericolo generico. Pericolo non

compreso tra le precedenti categorie.

Questo simbolo è utilizzato inoltre

sull’apparecchio per segnalare all’utente

di consultare le istruzioni descritte nel

presente manuale.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Wenn Sie sich über die Sicherheit eines

Verfahrens im unklaren sind, setzen Sie

sich, bevor Sie fortfahren, mit Ihrer

lokalen technischen

Unterstützungsorganisation für Thermo

Fisher Scientific San Jose Produkte in

Verbindung.

Si la sûreté d’un procédure est incertaine,

avant de continuer, contacter le plus

proche Service Clientèle pour les produits

de Thermo Fisher Scientific San Jose.

Cuando la certidumbre acerca de un

procedimiento sea dudosa, antes de

proseguir, pongase en contacto con la

Oficina de Asistencia Tecnica local para

los productos de Thermo Fisher Scientific

San Jose.

Quando e in dubbio la misura di sicurezza

per una procedura, prima di continuare, si

prega di mettersi in contatto con il

Servizio di Assistenza Tecnica locale per i

prodotti di Thermo Fisher Scientific San

Jose.

CAUTION Symbol CAUTION

Electric Shock: This instrument uses

high voltages that can cause personal

injury. Before servicing, shut down the

instrument and disconnect the instrument

from line power. Keep the top cover on

while operating the instrument. Do not

remove protective covers from PCBs.

Chemical: This instrument might contain

hazardous chemicals. Wear gloves when

handling toxic, carcinogenic, mutagenic,

or corrosive or irritant chemicals. Use

approved containers and proper

procedures to dispose waste oil.

Heat: Before servicing the instrument,

allow any heated components to cool.

Fire: Use care when operating the system

in the presence of flammable gases.

Eye Hazard: Eye damage could occur

from splattered chemicals or flying

particles. Wear safety glasses when

handling chemicals or servicing the

instrument.

General Hazard: A hazard is present that

is not included in the above categories.

Also, this symbol appears on the

instrument to refer the user to instructions

in this manual.

When the safety of a procedure is

questionable, contact your local Technical

Support organization for Thermo Fisher

Scientific San Jose Products.

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual ix

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi

About This Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

LightPipe Flowcell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Installation Checklist. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Unpacking and Inspecting Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Making Initial Instrument Preparations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Checking the Power Setting and Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Making Initial Rear Panel Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Ethernet Connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Analog Output Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Analog Output Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Unit ID Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Remote Communications Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Connecting and Installing the Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Powering On the Detector for the First Time . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Chapter 3 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19

Recommendations for Routine Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Tools Required . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Cleaning Detector External Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

LightPipe Flow Cell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Removing the LightPipe Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

Storing the LightPipe Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Installing the LightPipe Mounting Assembly. . . . . . . . . . . . . . . . . . . . . . . . . 24

Installing the LightPipe Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Contents

xSurveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Standard Analytical Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Removing the Standard Flow Cell Assembly . . . . . . . . . . . . . . . . . . . . . . . . . 27

Installing the Standard Analytical Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . 29

Cleaning Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Cleaning with Organic Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Cleaning with Nitric Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

Replacing the Lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

The Deuterium Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

The Tungsten Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Chapter 4 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

Detector-Related Problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Chapter 5 Accessories and Replaceable Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47

Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Optional Flow Cells for the UV/Vis Detector . . . . . . . . . . . . . . . . . . . . . . . . . . 48

Appendix A Surveyor Firmware Upgrade Utility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual xi

P

Preface

About This Guide

This Surveyor UV/Vis Plus Detector Hardware Manual provides you with information on how

to set up and maintain your Surveyor UV/Vis Plus detector.

Related Documentation

In addition to this guide, Thermo Fisher Scientific provides the following documents for the

Surveyor UV/Vis Plus Detector:

•Surveyor Plus Preinstallation Requirements Guide

•Surveyor Plus Getting Connected Guide

•Surveyor Plus Getting Started with ChromQuest

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and

other special notices appear in boxes.

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each

CAUTION notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of

data, or invalid test results; or might contain information that is critical for optimal

performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Preface

xii Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Contacting Us

There are several ways to contact Thermo Fisher Scientific for the information you need.

YTo contact Technical Support

Find software updates and utilities to download at www.mssupport.thermo.com.

YTo contact Customer Service for ordering information

YTo copy manuals from the Internet

Go to mssupport.thermo.com and click Customer Manuals in the left margin of the window.

YTo suggest changes to documentation or to Help

• Fill out a reader survey online at www.thermo.com/lcms-techpubs.

• Send an e-mail message to the Technical Publications Editor at

techpubs-lcms@thermofisher.com.

Phone 800-685-9535

Fax 561-688-8736

E-mail TechSupport.C+MS@thermofisher.com

Knowledge base www.thermokb.com

Phone 800-532-4752

Fax 561-688-8731

Web site www.thermo.com/ms

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual 1

1

Introduction

The Surveyor UV/Vis Plus Detector shown in Figure 1 is a member of the Surveyor Plus™

family of LC instruments.

This chapter provides an introduction to the Surveyor UV/Vis Plus Detector.

Figure 1. Surveyor UV/Vis Plus Detector

Contents

•Functional Description

•LightPipe Flowcell

•Specifications

1 Introduction

Functional Description

2Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Functional Description

The Surveyor UV/Vis Plus Detector is a full-featured, time-programmable,

variable-wavelength UV/Vis (ultraviolet / visible) absorbance detector. It operates in either the

single or dual wavelength mode in the UV or visible ranges.

The UV/Vis detector is a bench top unit for inclusion into the Surveyor LC System. It is

controlled remotely over an Ethernet communications link from a PC using ChromQuest

software.

The UV/Vis detector consists of a dual light source, an optical bench (see Figure 2), dual

photodiode detectors, and several printed circuit boards (PCBs).

Figure 2. The Surveyor UV/Vis Plus Detector optical bench

To provide a complete spectrum of ultraviolet and visible light, the detector uses a deuterium

lamp for the UV range (190-365 nm) and a tungsten lamp for the visible range

(366-800 nm). The lamps are protected by a cover with a special safety interlock to reduce the

possibility of human exposure to harmful UV light. A computer-controlled shutter selects

which lamp is used for sample analysis. The light from the selected lamp goes to a holographic

grating that disperses the light into a spectrum. The grating can be turned to select which

portion of the spectrum and which wavelength of light is used. The alignment of the grating

is controlled by a stepper motor for precise wavelength control. Absorbance linearity is

increased by a system of baffles that reduces stray light in the optical bench.

Tungsten

lamp

Deuterium

lamp

Sample

photodiode

Reference

photodiode

Lens

Flow cell

Beamsplitter

Shutter

Grating

1 Introduction

LightPipe Flowcell

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual 3

From the grating, the light travels to a fiber optic beamsplitter, where a portion of the light is

redirected to a reference photodiode. The signal from the reference photodiode is used to

compensate for changes in lamp intensity and helps to maintain consistent performance as the

lamp performance varies. From the beamsplitter, most of the light passes through a lens and

then on through the flow cell and onto the sample photodiode. The light falling on the

sample photodiode is then processed by the analog PC board and converted into data that can

be analyzed by the computer.

The available flow cell options include:

• Standard analytical flow cell with a 10 mm pathlength

• LightPipe flow cell with a 50 mm pathlength

Other flow cells can also be used in the detector. See Tabl e 3.

LEDs on the front of the module keep the user informed of power, communications, run, and

lamp status.

LightPipe Flowcell

The Surveyor UV/Vis Plus Detector can be ordered with a 50 mm LightPipe flowcell.

The internal bore of the LightPipe flowcell is 50 mm long and has a volume of 10 μL. The

5 cm optical pathlength produces a signal five times that of a conventional 1 cm flowcell,

while the low internal volume of 10 μL minimizes band broadening. A special low refractive

index coating of the internal bore ensures a high optical throughput and minimizes

short-term noise.

Mobile phase enters the LightPipe flowcell through a port in the bottom of the flowcell and

exits through a port in the top of the flowcell. See Figure 3. Directing the flow from the

bottom to the top of the flowcell reduces the development of air bubbles.

Figure 3. Schematic of LightPipe flowcell, showing the internal flowpath

Outlet port

Inlet port 50 mm flowpath

Alignment slot

1 Introduction

LightPipe Flowcell

4Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Sensitive optical fibers are exposed at both ends of the flowcell as shown in Figure 4. Thermo

Fisher Scientific ships the LightPipe flowcell with end caps to protect these optical fibers. See

Figure 5. Leave these protective end caps in place until you install the LightPipe flowcell.

Replace the protective end caps for storage if you remove the LightPipe flowcell from the

detector.

Figure 4. End of LightPipe flowcell, showing the sensitive optical fiber

Figure 5. LightPipe flowcell with protective end caps

CAUTION Do not touch the ends of the LightPipe flowcell. Doing so could damage the

sensitive optical fibers. If you must grasp the ends of the LightPipe flowcell, wear clean,

talc-free gloves.

Optical fiber

Tubing port

Protective end caps

for storage

1 Introduction

Specifications

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual 5

Specifications

Light Source: Deuterium and tungsten lamps, re-aligned

Wavelength Range: 190 nm to 800 nm

•D

2 Lamp: 190 to 365 nm

• W Lamp: 366 to 800 nm

Wavelength Accuracy: ±1 nm at 254 nm and 656 nm

Spectral Bandwidth: 6 nm

Absorbance Range: 0.0005 AUFS to +3.0 AUFS

Short Term Noise*: • 6 μAU/cm at 254 nm, 1 s rise time, single wavelength mode

(50 mm LightPipe flow cell)

• 20 μAU/cm at 254 nm, 1 s rise time, single wavelength mode

(10 mm standard analytical flow cell)

Drift*:•≤ 1 mAU/h at 254 nm and 546 nm

(50 mm LightPipe flow cell)

•≤ 0.1 mAU/h at 254 nm and 546 nm

(10 mm standard analytical flow cell)

Warm-up: 90 min to meet noise and drift specifications

Linearity: deviation ≤5% up to 2.0 AU at 256 nm

Scan Rate: 4, 5, 6.67, 10, or 20 Hz (user selectable)

Rise Time: 0, 0.1, 0.2, 0.5, 1, 2, 3, 4, or 5 s (user selectable)

Cell Dimensions: • 10 mm, 15 μL standard analytical

•50mm, 10 μL LightPipe

Cell Pressure Rating: 1000 psi

Analog Outputs (2): Unattenuated at 10 mV/AU, 100 mV/AU, or 1.0 V/AU

Remote Controls: Start, Zero, RJ45 Ethernet interface for ChromQuest

Dimensions: 18 cm (7.1 in.) ×36 cm (14.2 in.) ×47 cm (18.5 in.) (h × w × d)

Weight: 19.5 kg (43 lbs)

Power Requirements: 100/115 or 230 V ac; 50/60 Hz, 225 VA max.

Fuses: • 2.5 A at 100/115 V ac (slo-blo)

• 1.25 A at 230 V ac (slo-blo)

Operating Temperature: +10 to +30 °C

Storage Temperature: - 40 to +70 °C

Operating Humidity: 5% to 95% non-condensing relative humidity

Product Certification: CE, TUV-C/US, FCC (EMI)

*According to ASTM E1657-94 “Standard Practice for Testing Variable-Wavelength Photometric Detectors Used in Liquid Chromatography” (for

50 mm flow cell, 5 nm bandwidth, 2 sec rise time, and MeOH at 1 mL/min.).

Thermo Scientific Surveyor UV/Vis Plus Detector Hardware Manual 7

2

Installation

This chapter describes the initial installation of your Surveyor UV/Vis Plus Detector,

including connection to other chromatographic instrumentation. The Installation Checklist,

on the back of this page, is an abbreviated version of this chapter and can be used as a quick

reference of how to conduct a successful installation. Make a copy of the checklist and fill it

out when the installation is complete. Include the completed checklist in your maintenance

records.

Note Perform the installation in the sequence presented on the Installation Checklist and

detailed in this chapter.

Contents

•Installation Checklist

•Unpacking and Inspecting Instrument

•Making Initial Instrument Preparations

•Checking the Power Setting and Fuses

•Making Initial Rear Panel Connections

•Connecting and Installing the Flow Cell

•Powering On the Detector for the First Time

2 Installation

Installation Checklist

8Surveyor UV/Vis Plus Detector Hardware Manual Thermo Scientific

Installation Checklist

The following installation checklist is a brief summary of the steps that you need to complete

for the proper installation of your Surveyor UV/Vis Plus Detector:

Unpacking and Inspecting Instrument

Carefully remove the detector from the shipping container and inspect both the detector and

the packaging for any signs of damage. If you find any damage, save the shipping materials

and immediately contact the shipping company.

The shipping container should contain the following items:

• Surveyor UV/Vis Plus Detector

• Accessory kit

Detectors ordered with a standard 10 mm flow cell arrive with the flow cell installed in the

detector. Detectors ordered with the 50 mm LightPipe are shipped with the LightPipe

packaged in the accessory kit.

The accessory kit (P/N 60053-62004) contains the items listed in Tab le 1. Carefully check to

make sure you have received all the items listed on the packing list. If any items are missing,

contact your Thermo Fisher Scientific representative immediately.

UUnpack and inspect your instrument (page 8).

URead the safety notices in the preface of this manual.

UMake the initial instrument preparations (page 8).

UCheck the power settings and fuses (page 10).

UMake the initial rear panel connections (page 11)

UConnect and install the flow cell (page 15)

UPower on the detector for the first time (page 16)

UDownload the Surveyor UV/Vis Plus Detector firmware, if required (Appendix A,

“Surveyor Firmware Upgrade Utility.” )

This Surveyor UV/Vis Plus Detector was installed by:

(Name) (Date)

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